Melanositik lezyonlarda p16 ve CD117 ekspresyonunun önemi

Yıl 2021, Cilt: 7 Sayı: 2, 113 - 119, 30.08.2021

Sevil Karabağ Ayşegül İsal Arslan

https://doi.org/10.30569/adiyamansaglik.890978

Öz

Amaç: Bu çalışmada melanositik lezyonların p16 ve CD117 ekspresyon profillerini belirleyerek, melanomun diğer benign ya da potansiyel prekürsör melanositik lezyonlardan ayırıcı tanısını yapmayı kolaylaştıracak immün profillerini saptamayı amaçladık.
Gereç ve Yöntem: Seksen bir melanositik lezyon olgusuna immünohistokimya ile p16 ve CD117 uygulandı.
Bulgular: Melanom olgularında diğer benign ve prekürsör melanositik lezyonlara kıyasla anlamlı olarak p16 ekpresyon kaybı mevcuttur(p<0.05). Ayrıca displastik nevüs olgularında da intradermal nevüs olgularına göre anlamlı olarak p16 ekspresyonunda kayıp mevcuttur (p<0.01). CD117 ise intradermal nevüslerde eksprese olmaz iken, spitz nevüs, blue nevüs, invaziv melanom ve displastik nevüslerde ise yüksek oranda ekspresyon mevcuttur (p<0.01).
Sonuç: p16 ve CD117 belirteçlerinin birlikte kullanıldığı zaman, melanomları benign lezyonlardan ve benign lezyonları potansiyel prekürsör melanositik lezyonlardan ayırımda önemli belirteçler olarak katkı sağlayacağını düşünmekteyiz.

Anahtar Kelimeler

Melanositik tümör, İmmünohistokimya, P16, CD117

Destekleyen Kurum

Araştırmanın destekleyicisi bulunmamaktadır

The importance of p16 and CD117 expression in melanocytic lesions

Öz

Aim: The present study aims to determine the p16 and CD117 expression profiles of melanocytic lesions to investigate immune profiles that may facilitate differential diagnosis of melanoma from benign or potential precursor melanocytic lesions.
Materials and Methods: Immunohistochemistry for p16 and CD117 was applied in a total of 81 cases with melanocytic lesions.
Results: A significant loss of p16 expression was found in melanoma cases compared to benign and precursor melanocytic lesions (p<0.05). Moreover, a significant loss of p16 expression was also noted in cases of dysplastic nevus compared to those with intradermal nevus (p<0.01). While no CD117 expression was observed in intradermal nevi, high-level expression was seen in cases with Spitz nevus, blue nevus, invasive melanoma and dysplastic nevus (p<0.01).
Conclusion: We believe using p16 and CD117 together may provide an important marker combination to aid in distinguishing melanoma from benign lesions and benign lesions from potential precursor melanocytic lesions.

Anahtar Kelimeler

Melanocytic tumors, Immunohistochemistry, P16, CD117

Kaynakça

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