Ürolitiazisli hastalarda biyofilm oluşumu ve antibiyotik direnci: fenotipik ve genotipik değerlendirme

Yıl 2024, Cilt: 10 Sayı: 1, 16 - 23, 23.04.2024

Mohammed Munadi Mohammed Mohammed , Serhat Sirekbasan , Asal Aziz Tawfeeq

https://doi.org/10.30569/adiyamansaglik.1403447

Öz

Amaç: Ürolitiyazis, toplumu önemli ölçüde etkileyen yaygın bir halk sağlığı sorunudur. Bu çalışmanın amacı; ürolitiyazisli hastalarda gelişen üriner sistem enfeksiyonlarının yaygın etkenlerini saptamak ve bu kökenlerin biyofilm oluşturma yeteneğinin fenotipik ve moleküler olarak araştırılmasıdır.
Gereç ve Yöntem: Mayıs 2021 ile Kasım 2022 tarihleri arasında yürüttüğümüz çalışmamıza Kerkük Eğitim ve Araştırma Hastanesi'ne idrar yolu enfeksiyonu semptomları ile başvuran ve böbrek taşı tanısı alan toplam 100 hasta dahil edildi. Alınan idrar örneklerinden klinik olarak önemli bakterilerin tanımlanması için rutin konvansiyonel yöntemler kullanıldı. Saptanan kökenlerin biyofilm oluşumu mikroplak yöntemi ile incelendi.
Bulgular: En sık izole edilen etkenler Escherichia coli (n:36) ve Proteus mirabilis (n:17) olarak saptandı. E. coli kökenlerinin %89’unda, P. mirabilis kökenlerinin ise %94'ünde biyofilm oluşumu gözlendi.
Sonuç: Elde edilen sonuçlar, özellikle E. coli ve P. mirabilis kökenlerinde yüksek biyofilm oluşumu ve bu biyofilm oluşumuna ilişkin genlerin sıklıkla bulunması açısından önemlidir.

Anahtar Kelimeler

Ürolitiyazis, Biyofilm, Böbrek taşı, Direnç genleri

Etik Beyan

Çalışma için Kerkük Sağlık Dairesi Etik Kurulu’ndan onay alındı (Tarih: 1 Mart 2022, Sayı: 149). Çalışmamız Helsinki bildirisine uygun olarak yapıldı.

Destekleyen Kurum

Çalışmayı maddi olarak destekleyen kişi/kuruluş yoktur.

Teşekkür

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Biofilm formation and antibiotic resistance in patients with urolithiasis: assessment of phenotypic and genotypic

Öz

Aim: Urolithiasis is a common public health problem that significantly impacts the community. The objective of this study was to identify the prevalent pathogens responsible for urinary tract infections in patients with urolithiasis and investigate the biofilm forming ability of these strains phenotypically and molecularly.
Materials and Methods: A total of 100 patients who presented to Kirkuk Training and Research Hospital with symptoms of urinary tract infection and were diagnosed with kidney stones were included in our study conducted between May 2021 and November 2022. Clinically significant bacteria from urine samples were identified using routine conventional methods. Biofilm formation of the identified strains was examined by microplate method.
Results: The most frequently isolated agents were Escherichia coli (n:36) and Proteus mirabilis (n:17). Biofilm formation was detected in 89% of E. coli strains and 94% of P. mirabilis strains.
Conclusion: The results obtained are important in terms of high biofilm formation, especially in E. coli and P. mirabilis strains, and the frequent presence of genes related to this biofilm formation.

Anahtar Kelimeler

Urolithiasis, Biofilm, Kidney stones, Resistance genes

Etik Beyan

Ethical committee approval was received from the Ethics Committee of Kirkuk Health Office (Date: March 1, 2022, Number: 149). The study was conducted under the principles of the Declaration of Helsinki.

Destekleyen Kurum

No person/organization is supporting this study financially.

Teşekkür

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Kaynakça

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