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Evaluation of The Antimicrobial and Antioxidant Activity Potential of Salvia Officinalis L. Essential Oil

Yıl 2024, Cilt: 21 Sayı: 1, 89 - 94, 30.06.2024
https://doi.org/10.25308/aduziraat.1451479

Öz

This study set out to determine the effects of Salvia officinalis L., commonly known as sage, on the chemical composition of the essential oil, its free radical scavenging activity (IC50), ferric-reducing antioxidant power (FRAP) value, and antimicrobial properties. According to the results of the GC-MS analysis, 3-thujen-2-one, (26,21%), (+)-2-bornanone (16.47%), and 1,8-cineole (15.09%) were the compounds with the highest percentage in the sage essential oil. In addition, the essential oil showed high antioxidant activity, providing an IC50 value of 5.507 ± 0.723 mg/ml and a FRAP value of 2.129 ± 0.136 mM/g. The findings of the agar disc diffusion test demonstrated that the highest inhibition zones were against Bacillus cereus NRRL B3711 and Bacillus subtilis PY79 bacteria, whereas the lowest antimicrobial effect was shown against Listeria monocytogenes ATCC 19115 and Escherichia coli ATCC 25922. Similarly, sage essential oil showed the highest antimicrobial effect in the broth dilution test with a minimum inhibitory concentration (MIC) value of 2.94 mg/ml against Bacillus cereus NRRL B3711, while its effectiveness against Staphylococcus spp., Enterococcus faecalis ATCC 29212, and Salmonella typhimurium ATCC 14028 bacteria was the lowest with a MIC value of 23.5 mg/ml.

Kaynakça

  • Abadi A, Hassani A (2013) Essential Oil Composition and Antioxidant Activity of Marrubium vulgare L. Growing Wild in Eastern Algeria. International Letters of Chemistry, Physics and Astronomy 9(1): 17-24.
  • Afonso AF, Pereira OR, Fernandes Â, Calhelha RC, Silva AM, Ferreira IC, Cardoso SM (2019) Phytochemical Composition and Bioactive Effects of Salvia africana, Salvia officinalis ‘Icterina’ and Salvia Mexicana Aqueous Extracts. Molecules 24(23): 4327.
  • Assaggaf HM, Naceiri Mrabti H, Rajab BS, Attar AA, Alyamani RA, Hamed M, El Omari N, El Menyiy N, Hazzoumi Z, BenaliT, Al-Mijalli SH, Zengin G,Al Dhaberi Y, Eid AH, Bouyahya A (2022) Chemical Analysis and Investigation of Biological Effects of Salvia officinalis Essential Oils at Three Phenological Stages. Molecules 27(16): 5157.
  • Bağdat RB (2006) Tıbbi ve Aromatik Bitkilerin Kullanim alanları, Tıbbi Adaçayı (Salvia officinalis L.) ve Ülkemizde Kekik Adıyla Bilinen Türlerin Yetiştirme Teknikleri. Tarla Bitkileri Merkez Araştırma Enstitüsü Dergisi 15(1-2): 19-28.
  • Baliyan S, Mukherjee R, Priyadarshini A, Vibhuti A, Gupta A, Pandey RP, Chang CM (2022) Determination of Antioxidants by DPPH Radical Scavenging Activity and Quantitative Phytochemical Analysis of Ficus religiosa. Molecules 27(4): 1326.
  • Bonesi M, Loizzo MR, Acquaviva R, Malfa GA, Aiello F, Tundis R (2017) Anti-inflammatory and Antioxidant Agents from Salvia genus (Lamiaceae): An Assessment of the Current State of Knowledge. Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry-Anti-Inflammatory and Anti-Allergy Agents) 16(2): 70-86.
  • Boufadi MY, Keddari S, Moulai-Hacene F, Sara CHAA (2021) Chemical Composition, Antioxidant and Anti-Inflammatory Properties of Salvia officinalis Extract from Algeria. Pharmacognosy Journal 13(2).
  • Boyle W (1955) Spices and Essential Oils as Preservatives. The American Perfumer and Essential Oil Review 66(1): 25-28.
  • Burt S (2004) Essential Oils: Their Antibacterial Properties and Potential Applications in Foods—A Review. International Journal of Food Microbiology 94(3): 223-253.
  • Chouhan S, Sharma K,Guleria S (2017) Antimicrobial Activity of Some Essential Oils—Present Status and Future Perspectives. Medicines 4(3): 58.
  • Cuendet M, Hostettmann K, Potterat O, Dyatmiko W (1997) Iridoid Glucosides with Free Radical Scavenging Properties from Fagraeablumei. Helvetica Chimica Acta 80(4): 1144-1152.
  • de Sousa Barros A, de Morais SM, Ferreira PAT, Vieira ÍGP, Craveiro AA, dos Santos Fontenelle RO, de Menezes JEA, da Silva FWF, de Sousa HA (2015) Chemical Composition and Functional Properties of Essential Oils from Mentha Species. Industrial Crops and Products 76: 557-564.
  • Elshamy A, Abd-ElGawad A, Mohamed T, El Gendy AEN, Abd El Aty AA, Saleh I, Moustafa MF, Hussien TA, Pare PW, Hegazy MEF (2021) Extraction Development for Antimicrobial and Phytotoxic Essential Oils From Asteraceae Species: Achillea fragrantissima, Artemisia judaica and Tanacetum sinaicum. Flavour and Fragrance Journal 36(3): 352-364.
  • Farhat, MB, Chaouch-Hamada, R, Sotomayor, JA, Landoulsi, A, Jordán, MJ (2014) Antioxidant Potential of Salvia officinalis L. Residues as Affected by the Harvesting Time. Industrial Crops and Products 54:78-85.
  • Gedikoğlu A (2022) Antimicrobial and Antioxidant Activities of Commercialized Turkish Propolis Extract, and Application to Beef Meatballs. Turkish Journal of Agriculture-Food Science and Technology 10(10): 2021-2029.
  • Gedikoğlu A, Çıkrıkcı Erünsal S (2023) Characterization of a Thymbra spicata Essential Oil–Pectin Nanoemulsion, and Antimicrobial Activity Against Foodborne Pathogenic Bacteria. Journal of Food Measurement and Characterization17:3195-3206.
  • Gedikoğlu A, Sökmen M, Çivit A (2019). Evaluation of Thymus vulgaris and Thymbra spicata Essential Oils and Plant Extracts for Chemical Composition, Antioxidant, and Antimicrobial Properties. Food Science & Nutrition 7(5): 1704-1714.
  • Gökoğlu N (2018) Novel Natural Food Preservatives and Applications in Seafood Preservation: A Review Journal of the Science of Food and Agriculture 99:2068-2077.
  • Gulcin İ, Alwasel SH (2023) DPPH Radical Scavenging Assay. Processes 11(8): 2248.
  • Ilic Z, Kevrešan Ž, Šunić L, Stanojević L, Milenkovic L, Stanojević J, Milenkovic A, Cvetkovic D (2023) Chemical Profiling and Antioxidant Activity of Wild and Cultivated Sage (Salvia officinalis L.) Essential Oil. Horticulturae 9(6): 624.
  • Jakovljević M; Jokić S, Molnar M, Jašić M, Babić J, Jukić H, Banjari I (2019) Bioactive Profile of Various Salvia officinalis L. Preparations. Plants 8: 55.
  • Khiya Z, Hayani M, Gamar A, Kharchouf S, Amine S, Berrekhis F, Bouzoubae A, Zair T, El Hilali F (2019) Valorization of the Salvia officinalis L. of the Morocco Bioactive Extracts: Phytochemistry, Antioxidant Activity and Corrosion Inhibition. Journal of King Saud University-Science 31(3): 322-335.
  • Khiya Z, Oualcadi Y, Gamar A, Berrekhis F, Zair T, Hilali FE (2021) Correlation of Total Polyphenolic Content With Antioxidant Activity of Hydromethanolic Extract And Their Fractions of the Salvia officinalis Leaves from Different Regions of Morocco. Journal of Chemistry1-11.
  • Koyama S, Heinbockel T (2020) The Effects of Essential Oils and Terpenes in Relation to Their Routes of Intake and Application. International Journal of Molecular Sciences 21(5): 1558.
  • Kumari PK, Akhila S, Rao YS, Devi BR (2019) Alternative to Artificial Preservatives. Systematic Reviews in Pharmacy 10(1): 99-102.
  • Moghimi R, Aliahmadi A, McClements DJ, Rafati H (2017) Nanoemulsification of Salvia officinalis Essential Oil; The Impact on The Antibacterial Activity in Liquid and Vapour Phase. Journal of Bionanoscience 11(1): 80-86.
  • Reyes-Jurado F, Franco-Vega A, Ramírez-Corona N, Palou E, López-Malo A (2015) Essential Oils: Antimicrobial Activities, Extraction Methods, and Their Modeling. Food Engineering Reviews 7: 275-297.
  • Riahi L, Chograni H, Elferchichi M, Zaouali Y, Zoghlami N,Mliki A (2013) Variations in Tunisian Wormwood Essential Oil Profiles and Phenolic Contents Between Leaves and Flowers and Their Effects on Antioxidant Activities. Industrial Crops and Products 46:290-296.
  • Wali AF, Hamad EA, Khazandar AA, Al-Azzawi AM, Sarheed OA, Menezes GA, Alam A (2019) Antimicrobial and in vitro Antioxidant Activity of Salvia officinalis L. Against Various Re-Emergent Multidrug Resistance Microbial Pathogens. Annals of Phytomedicine 8(2): 115-120.
  • Wińska K, Mączka W. Łyczko J, Grabarczyk M, Czubaszek A, Szumny A (2019) Essential Oils as Antimicrobial Agents—Myth or Real Alternative?. Molecules 24(11): 2130.

Salvia officinalis L. Esansiyel Yağının Antimikrobiyal ve Antioksidan Aktivite Potansiyelinin Değerlendirilmesi

Yıl 2024, Cilt: 21 Sayı: 1, 89 - 94, 30.06.2024
https://doi.org/10.25308/aduziraat.1451479

Öz

Bu çalışma, Salvia officinalis L., yaygın adıyla adaçayı bitkisinden elde edilen uçucu yağın kimyasal bileşimini, serbest radikal temizleme aktivitesini (IC50), ferrik indirgeyici antioksidan güç (FRAP) değerini ve antimikrobiyal özellikler üzerindeki etkisini belirlemeyi amaçlamıştır. GC-MS analizi sonuçlarına göre, sırasıyla 3-thujen-2-bir (%26,21), (+)-2-bornanone (%16,47) ve 1,8-sineol’ün (%15,09) ada çayı uçucu yağında en yüksek yüzdeye sahip bileşikler olduğu görülmüştür. Ayrıca, uçucu yağ yüksek antioksidan aktivitesi göstermiş olup, IC50 değeri 5,507 ± 0,723 mg/ml ve FRAP değeri 2,129 ± 0,136 mM/g olarak bulunmuştur. Agar disk difüzyon testinin bulguları, en yüksek inhibisyon bölgelerinin Bacillus cereus NRRL B3711 ve Bacillus subtilis PY79 bakterilerine karşı olduğu görülürken, en düşük antibakteriyel etkinin Listeria monocytogenes ATCC 19115 ve Escherichia coli ATCC 25922 bakterilerine karşı olduğu tespit edilmiştir. Benzer şekilde, ada çayı uçucu yağı mikro tüp dilüsyon testinde en yüksek antibakteriyel etkiyi Bacillus cereus NRRL B3711 bakterisine karşı 2,94 mg/ml MİK (minimum inhibisyon konsantrasyonu) değeri ile göstermiş olup, Staphyloccoccus spp., Enterococcus faecalis ATCC 29212, ve Salmonella typhimurium ATCC 14028 bakterilerine karşı 23,5 mg/ml MİK değeri ile etkinliği en düşük çıkmıştır.

Etik Beyan

Yazarlar Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi tarafından beyan edilen etik kurallara uymuşlardır.

Destekleyen Kurum

Konya Gıda ve Tarım Üniversitesi

Teşekkür

Konya Şeker Atıştırmalık farikasından Mensur Gün`e GC-MS analizi sırasındaki yardımlarından dolayı teşekkür ederiz.

Kaynakça

  • Abadi A, Hassani A (2013) Essential Oil Composition and Antioxidant Activity of Marrubium vulgare L. Growing Wild in Eastern Algeria. International Letters of Chemistry, Physics and Astronomy 9(1): 17-24.
  • Afonso AF, Pereira OR, Fernandes Â, Calhelha RC, Silva AM, Ferreira IC, Cardoso SM (2019) Phytochemical Composition and Bioactive Effects of Salvia africana, Salvia officinalis ‘Icterina’ and Salvia Mexicana Aqueous Extracts. Molecules 24(23): 4327.
  • Assaggaf HM, Naceiri Mrabti H, Rajab BS, Attar AA, Alyamani RA, Hamed M, El Omari N, El Menyiy N, Hazzoumi Z, BenaliT, Al-Mijalli SH, Zengin G,Al Dhaberi Y, Eid AH, Bouyahya A (2022) Chemical Analysis and Investigation of Biological Effects of Salvia officinalis Essential Oils at Three Phenological Stages. Molecules 27(16): 5157.
  • Bağdat RB (2006) Tıbbi ve Aromatik Bitkilerin Kullanim alanları, Tıbbi Adaçayı (Salvia officinalis L.) ve Ülkemizde Kekik Adıyla Bilinen Türlerin Yetiştirme Teknikleri. Tarla Bitkileri Merkez Araştırma Enstitüsü Dergisi 15(1-2): 19-28.
  • Baliyan S, Mukherjee R, Priyadarshini A, Vibhuti A, Gupta A, Pandey RP, Chang CM (2022) Determination of Antioxidants by DPPH Radical Scavenging Activity and Quantitative Phytochemical Analysis of Ficus religiosa. Molecules 27(4): 1326.
  • Bonesi M, Loizzo MR, Acquaviva R, Malfa GA, Aiello F, Tundis R (2017) Anti-inflammatory and Antioxidant Agents from Salvia genus (Lamiaceae): An Assessment of the Current State of Knowledge. Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry-Anti-Inflammatory and Anti-Allergy Agents) 16(2): 70-86.
  • Boufadi MY, Keddari S, Moulai-Hacene F, Sara CHAA (2021) Chemical Composition, Antioxidant and Anti-Inflammatory Properties of Salvia officinalis Extract from Algeria. Pharmacognosy Journal 13(2).
  • Boyle W (1955) Spices and Essential Oils as Preservatives. The American Perfumer and Essential Oil Review 66(1): 25-28.
  • Burt S (2004) Essential Oils: Their Antibacterial Properties and Potential Applications in Foods—A Review. International Journal of Food Microbiology 94(3): 223-253.
  • Chouhan S, Sharma K,Guleria S (2017) Antimicrobial Activity of Some Essential Oils—Present Status and Future Perspectives. Medicines 4(3): 58.
  • Cuendet M, Hostettmann K, Potterat O, Dyatmiko W (1997) Iridoid Glucosides with Free Radical Scavenging Properties from Fagraeablumei. Helvetica Chimica Acta 80(4): 1144-1152.
  • de Sousa Barros A, de Morais SM, Ferreira PAT, Vieira ÍGP, Craveiro AA, dos Santos Fontenelle RO, de Menezes JEA, da Silva FWF, de Sousa HA (2015) Chemical Composition and Functional Properties of Essential Oils from Mentha Species. Industrial Crops and Products 76: 557-564.
  • Elshamy A, Abd-ElGawad A, Mohamed T, El Gendy AEN, Abd El Aty AA, Saleh I, Moustafa MF, Hussien TA, Pare PW, Hegazy MEF (2021) Extraction Development for Antimicrobial and Phytotoxic Essential Oils From Asteraceae Species: Achillea fragrantissima, Artemisia judaica and Tanacetum sinaicum. Flavour and Fragrance Journal 36(3): 352-364.
  • Farhat, MB, Chaouch-Hamada, R, Sotomayor, JA, Landoulsi, A, Jordán, MJ (2014) Antioxidant Potential of Salvia officinalis L. Residues as Affected by the Harvesting Time. Industrial Crops and Products 54:78-85.
  • Gedikoğlu A (2022) Antimicrobial and Antioxidant Activities of Commercialized Turkish Propolis Extract, and Application to Beef Meatballs. Turkish Journal of Agriculture-Food Science and Technology 10(10): 2021-2029.
  • Gedikoğlu A, Çıkrıkcı Erünsal S (2023) Characterization of a Thymbra spicata Essential Oil–Pectin Nanoemulsion, and Antimicrobial Activity Against Foodborne Pathogenic Bacteria. Journal of Food Measurement and Characterization17:3195-3206.
  • Gedikoğlu A, Sökmen M, Çivit A (2019). Evaluation of Thymus vulgaris and Thymbra spicata Essential Oils and Plant Extracts for Chemical Composition, Antioxidant, and Antimicrobial Properties. Food Science & Nutrition 7(5): 1704-1714.
  • Gökoğlu N (2018) Novel Natural Food Preservatives and Applications in Seafood Preservation: A Review Journal of the Science of Food and Agriculture 99:2068-2077.
  • Gulcin İ, Alwasel SH (2023) DPPH Radical Scavenging Assay. Processes 11(8): 2248.
  • Ilic Z, Kevrešan Ž, Šunić L, Stanojević L, Milenkovic L, Stanojević J, Milenkovic A, Cvetkovic D (2023) Chemical Profiling and Antioxidant Activity of Wild and Cultivated Sage (Salvia officinalis L.) Essential Oil. Horticulturae 9(6): 624.
  • Jakovljević M; Jokić S, Molnar M, Jašić M, Babić J, Jukić H, Banjari I (2019) Bioactive Profile of Various Salvia officinalis L. Preparations. Plants 8: 55.
  • Khiya Z, Hayani M, Gamar A, Kharchouf S, Amine S, Berrekhis F, Bouzoubae A, Zair T, El Hilali F (2019) Valorization of the Salvia officinalis L. of the Morocco Bioactive Extracts: Phytochemistry, Antioxidant Activity and Corrosion Inhibition. Journal of King Saud University-Science 31(3): 322-335.
  • Khiya Z, Oualcadi Y, Gamar A, Berrekhis F, Zair T, Hilali FE (2021) Correlation of Total Polyphenolic Content With Antioxidant Activity of Hydromethanolic Extract And Their Fractions of the Salvia officinalis Leaves from Different Regions of Morocco. Journal of Chemistry1-11.
  • Koyama S, Heinbockel T (2020) The Effects of Essential Oils and Terpenes in Relation to Their Routes of Intake and Application. International Journal of Molecular Sciences 21(5): 1558.
  • Kumari PK, Akhila S, Rao YS, Devi BR (2019) Alternative to Artificial Preservatives. Systematic Reviews in Pharmacy 10(1): 99-102.
  • Moghimi R, Aliahmadi A, McClements DJ, Rafati H (2017) Nanoemulsification of Salvia officinalis Essential Oil; The Impact on The Antibacterial Activity in Liquid and Vapour Phase. Journal of Bionanoscience 11(1): 80-86.
  • Reyes-Jurado F, Franco-Vega A, Ramírez-Corona N, Palou E, López-Malo A (2015) Essential Oils: Antimicrobial Activities, Extraction Methods, and Their Modeling. Food Engineering Reviews 7: 275-297.
  • Riahi L, Chograni H, Elferchichi M, Zaouali Y, Zoghlami N,Mliki A (2013) Variations in Tunisian Wormwood Essential Oil Profiles and Phenolic Contents Between Leaves and Flowers and Their Effects on Antioxidant Activities. Industrial Crops and Products 46:290-296.
  • Wali AF, Hamad EA, Khazandar AA, Al-Azzawi AM, Sarheed OA, Menezes GA, Alam A (2019) Antimicrobial and in vitro Antioxidant Activity of Salvia officinalis L. Against Various Re-Emergent Multidrug Resistance Microbial Pathogens. Annals of Phytomedicine 8(2): 115-120.
  • Wińska K, Mączka W. Łyczko J, Grabarczyk M, Czubaszek A, Szumny A (2019) Essential Oils as Antimicrobial Agents—Myth or Real Alternative?. Molecules 24(11): 2130.
Toplam 30 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Gıda Mühendisliği
Bölüm Araştırma
Yazarlar

Afranur Özçoban 0000-0001-6971-451X

Ayça Gedikoğlu 0000-0002-5105-141X

Yayımlanma Tarihi 30 Haziran 2024
Gönderilme Tarihi 12 Mart 2024
Kabul Tarihi 6 Haziran 2024
Yayımlandığı Sayı Yıl 2024 Cilt: 21 Sayı: 1

Kaynak Göster

APA Özçoban, A., & Gedikoğlu, A. (2024). Salvia officinalis L. Esansiyel Yağının Antimikrobiyal ve Antioksidan Aktivite Potansiyelinin Değerlendirilmesi. Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi, 21(1), 89-94. https://doi.org/10.25308/aduziraat.1451479
AMA Özçoban A, Gedikoğlu A. Salvia officinalis L. Esansiyel Yağının Antimikrobiyal ve Antioksidan Aktivite Potansiyelinin Değerlendirilmesi. ADÜ ZİRAAT DERG. Haziran 2024;21(1):89-94. doi:10.25308/aduziraat.1451479
Chicago Özçoban, Afranur, ve Ayça Gedikoğlu. “Salvia Officinalis L. Esansiyel Yağının Antimikrobiyal Ve Antioksidan Aktivite Potansiyelinin Değerlendirilmesi”. Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi 21, sy. 1 (Haziran 2024): 89-94. https://doi.org/10.25308/aduziraat.1451479.
EndNote Özçoban A, Gedikoğlu A (01 Haziran 2024) Salvia officinalis L. Esansiyel Yağının Antimikrobiyal ve Antioksidan Aktivite Potansiyelinin Değerlendirilmesi. Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi 21 1 89–94.
IEEE A. Özçoban ve A. Gedikoğlu, “Salvia officinalis L. Esansiyel Yağının Antimikrobiyal ve Antioksidan Aktivite Potansiyelinin Değerlendirilmesi”, ADÜ ZİRAAT DERG, c. 21, sy. 1, ss. 89–94, 2024, doi: 10.25308/aduziraat.1451479.
ISNAD Özçoban, Afranur - Gedikoğlu, Ayça. “Salvia Officinalis L. Esansiyel Yağının Antimikrobiyal Ve Antioksidan Aktivite Potansiyelinin Değerlendirilmesi”. Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi 21/1 (Haziran 2024), 89-94. https://doi.org/10.25308/aduziraat.1451479.
JAMA Özçoban A, Gedikoğlu A. Salvia officinalis L. Esansiyel Yağının Antimikrobiyal ve Antioksidan Aktivite Potansiyelinin Değerlendirilmesi. ADÜ ZİRAAT DERG. 2024;21:89–94.
MLA Özçoban, Afranur ve Ayça Gedikoğlu. “Salvia Officinalis L. Esansiyel Yağının Antimikrobiyal Ve Antioksidan Aktivite Potansiyelinin Değerlendirilmesi”. Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi, c. 21, sy. 1, 2024, ss. 89-94, doi:10.25308/aduziraat.1451479.
Vancouver Özçoban A, Gedikoğlu A. Salvia officinalis L. Esansiyel Yağının Antimikrobiyal ve Antioksidan Aktivite Potansiyelinin Değerlendirilmesi. ADÜ ZİRAAT DERG. 2024;21(1):89-94.