Vitiligoda Dinamik Tiyol/Disülfid Homeostazın Rolü

Yıl 2022, Cilt: 6 Sayı: 1, 93 - 97, 22.04.2022

Bilal İlanbey , Emine Müge Acar

https://doi.org/10.46332/aemj.941512

Öz

Amaç: Vitiligo, melanositlerin progressif yıkımı sonucu oluşan pigment kaybı bozukluğudur. Melanosit kaybının patofizyolojisi günümüzde tam olarak çözülememiştir. Nedenler arasında oksidatif stres de yer almaktadır. Dinamik tiyol/disülfid homeostazı vücudun oksidan-antioksidan dengesinin önemli bir belirtecidir. Bu çalışmada vitiligo hastalarında sağlıklı bireylere göre tiyol/disülfid dengesini değerlendirdik.
Araçlar ve Yöntem: Vitiligolu 64 hasta ile 34 sağlıklı bireyin serumunda total tiyol ve native tiyol düzeyleri ile paraoxonaz1 (PON1) aktivitesi spektrofotometrik yöntemle ölçüldü. Serum disülfid düzeyleri ise hesaplamayla bulundu.
Bulgular: Vitiligolu hastaların serumunda sağlıklı kişilere göre total ve native tiyol düzeyleri yüksekti (her ikisi de p<0.001). Serum disülfid düzeylerinde ise her iki grup arasında istatistiksel olarak anlamlı fark yoktu.
Sonuç: Vitiligolu hastalarda tiyol/disülfid dengesinin yönü tiyol yönüne kaymıştır. Tiyol artışı hastalığın patofizyolojisinde görülen melanin kaybının sorumlusu olabilir. 

Anahtar Kelimeler

Tiyol/disülfid homeostazisi, vitiligo, oksidatif stres

Destekleyen Kurum

Kırşehir Ahi Evran Üniversitesi Bilimsel Araştırmalar Projeleri Koordinatörlüğü

Proje Numarası

TIP.A4.18.004

Teşekkür

Kırşehir Ahi Evran Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğüne projeye maddi destekleri için teşekkür ederiz.

Investigation of TheEffects of Trpc4/5 Inhibitor M084 in Experimental Alzheimer’s-LikeDementia Model

Öz

Purpose: Vitiligo is a disorder of pigment loss caused by the progressive destruction of melanocytes. The pathophysiology of melanocyte loss has not been fully elucidated today. Oxidative stress is also among the causes. Dynamic thiol/disulfide homeostasis is an essential indicator of the body's oxidant-antioxidant balance. In this study, we evaluated the thiol/disulfide balance in vitiligo patients compared to healthy individuals.

Materials and Methods: Total thiol and native thiol levels and paraoxonase1 (PON1) activity were measured using a spectrophotometric method in the serum of 64 patients with vitiligo and 34 healthy individuals. Serum disulfide levels were calculated by calculation.

Results: Total and native thiol levels were higher in the serum of patients with vitiligo compared to healthy individuals (both p<0.001). There was no statistically significant difference in serum disulfide levels between the two groups.

Conclusion: The thiol/disulfide balance direction has shifted to the thiol direction in patients with vitiligo. Thiol increase may be responsible for the loss of melanin seen in the pathophysiology of the disease.

Anahtar Kelimeler

Thiol/disulfide homeostasis, vitiligo, oxidative stress

Proje Numarası

TIP.A4.18.004

Kaynakça

• 1. Taieb A, Picardo M. Clinical practice. Vitiligo. N Engl J Med. 2009;360(2):160-169.
• 2. Boniface K, Seneschal J, Picardo M, Taieb A. Vitiligo: Focus on Clinical Aspects, Immunopathogenesis, and Therapy. Clin Rev Allergy Immunol. 2018;54(1):52-67.
• 3. Mohammed GF, Gomaa AH, Al-Dhubaibi MS. Highlights in pathogenesis of vitiligo. World J Clin Cases. 2015;3(3):221-230.
• 4. Speeckaert R, van Geel N. Vitiligo: An Update on Pathophysiology and Treatment Options. Am J Clin Dermatol. 2017;18(6):733-744.
• 5. Chen J, Li S, Li C. Mechanisms of melanocyte death in vitiligo. Med Res Rev. 2021;41(2):1138-1166.
• 6. Circu ML, Aw TY. Reactive oxygen species, cellular redox systems, and apoptosis. Free Radical Biology and Medicine. 2010;48(6):749-762.
• 7. Ates I, Kaplan M, Yuksel M, et al. Determination of thiol/disulphide homeostasis in type 1 diabetes mellitus and the factors associated with thiol oxidation. Endocrine. 2016;51(1):47-51.
• 8. Ayar G, Sahin S, Yazici MU, Neselioglu S, Erel O, Bayrakci US. Effects of Hemodialysis on Thiol-Disulphide Homeostasis in Critically Ill Pediatric Patients with Acute Kidney Injury. Biomed Res Int. 2018;(2018):1-6.
• 9. Erzin G, Kotan VO, Topcuoglu C, et al. Thiol/disulphide homeostasis in bipolar disorder. Psychiatry Res. 2018;(261): 237-242.
• 10. Gumusyayla S, Vural G, Bektas H, Deniz O, Neselioglu S, Erel O. A novel oxidative stress marker in patients with Alzheimer's disease: dynamic thiol-disulphide homeostasis. Acta Neuropsychiatr. 2016;28(6):315-320.
• 11. Nar R, Çalış AG. Assessment of dynamic thiol/disulfide homeostasis in patients with asthma. Journal of laboratory medicine. 2018;42(3):99-104.
• 12. Sivri S, Kasapkara HA, Polat M, et al. Dynamic thiol/disulphide homeostasis and its prognostic value in patients with non-ST elevation-acute coronary syndromes. Kardiol Pol. 2018;76(2):426-432.
• 13. Rorsman H, Albertsson E, Edholm L, Hansson C, Ogren L, Rosengren E. Thiols in the melanocyte. Pigment Cell Research. 1988;1(1):54-60.
• 14. Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem. 2014;47(18): 326-332.
• 15. Turell L, Radi R, Alvarez B. The thiol pool in human plasma: the central contribution of albumin to redox processes. Free Radic Biol Med. 2013;(65):244-253.
• 16. Jones DP, Liang Y. Measuring the poise of thiol/disulfide couples in vivo. Free Radic Biol Med. 2009;47(10):1329-1338.
• 17. Wang Y, Li S, Li C. Perspectives of New Advances in the Pathogenesis of Vitiligo: From Oxidative Stress to Autoimmunity. Med Sci Monit. 2019;(25):1017-1023.
• 18. Briganti S, Caron-Schreinemachers AL, Picardo M, Westerhof W. Anti-oxidant defence mechanism in vitiliginous skin increases with skin type. J Eur Acad Dermatol Venereol. 2012;26(10):1212-1219.
• 19. Di Dalmazi G, Hirshberg J, Lyle D, Freij JB, Caturegli P. Reactive oxygen species in organ-specific autoimmunity. Auto Immun Highlights. 2016;7(1):1-11.
• 20. Jara J, Aroca P, Solano F, Martinez J, Lozano J. The role of sulfhydryl compounds in mammalian melanogenesis: the effect of cysteine and glutathione upon tyrosinase and the intermediates of the pathway. Biochimica et Biophysica Acta (BBA)-General Subjects. 1988;967(2):296-303.
• 21. Jain A, Mal J, Mehndiratta V, Chander R, Patra SK. Study of oxidative stress in vitiligo. Indian J Clin Biochem. 2011;26(1):78-81.
• 22. Akoglu G, Neselioglu S, Karaismailoglu E, Aktas A, Erel O. Plasma Thiol Levels are Associated with Disease Severity in Nonsegmental Vitiligo. Indian J Dermatol. 2018;63(4):323-327.
• 23. Aksoy M, Celik H. Dynamic thiol/disulphide homeostasis in vitiligo patients. Postepy Dermatol Alergol. 2018;35(5):498-501.
• 24. Pektaş G, Pektaş SD, Öztekin A, et al. Dynamic thiol/disulfide homeostasis in serum of patients with generalized vitiligo. Arch Biol Sci. 2019;71(1):55-62.
• 25. El-Farargy SM, Ghanayem NM, Farag EK. Serum paraoxonase-1 levels as an indicator of oxidative stress in patients with vitiligo. Menoufia Medical Journal. 2021;34(1):40-42.