Development and Analysis of Metabolic Syndrome Feeds for Laboratory Animals (Rats and Mice)
Öz
Feeding a high-fat diet causes obesity and metabolic disorders in rodents that resemble human metabolic syndrome. The aim of this study was the preparation and characterization of casein-based high-fat feeds to induce metabolic syndrome in laboratory animals (rats and mice). In this study, three different concentrations of high-fat feeds (24, 35 and 45%), which are stable, non-pollinating and shedding, were developed in pellet form. The parameters used in the characterization of feeds were established within the specific features required for these feeds to achieve their usage goals. To determine various components and properties of prepared feeds; In addition to physical, chemical and microbiological analyses, in-vivo nutritional tests were carried out by comparing them with imported commercial products developed for the same purposes. The results showed that the products obtained were in accordance with the production targets and were like the analysis values of the imported feeds compared. In conclusion, the study revealed that locally developed casein-based high-fat pellet feeds can be used reliably in studies of various companies and research centers to create obesity and metabolic disorders in experimental animals.
Anahtar Kelimeler
High-fat feed, obesity, metabolic syndrome diets, laboratory animals.
Proje Numarası
KOSGEB - Laboratuvar hayvanlarında (rat ve fare) obesite, diabet, metabolik bozukluk hastalıkları oluşturan kazein bazlı yüksek yağlı emülsiyon tipi yemlerin üretimi
Kaynakça
- Aydin, S., Aksoy, A., Aydin, S., Kalayci, M., Yilmaz, M., Kuloglu, T., Citil, C. ve Catak, Z. (2014). Today’s and yesterday’s of pathophysiology: Biochemistry of metabolic syndrome and animal models. Nutrition, 30(1), 1–9. https://doi.org/10.1016/j.nut.2013.05.013
- Bellush, L. L. ve Rowland, N. E. (1986). Dietary self-selection in diabetic rats: An overview. Brain Research Bulletin, 17(5), 653–661. https://doi.org/10.1016/0361-9230(86)90197-8
- Bhatti, J. S., Bhatti, G. K. ve Reddy, P. H. (2017). Mitochondrial dysfunction and oxidative stress in metabolic disorders — A step towards mitochondria based therapeutic strategies. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1863(5), 1066–1077. https://doi.org/10.1016/j.bbadis.2016.11.010
- Botsoglou, N. A., Fletouris, D. J., Papageorgiou, G. E., Vassilopoulos, V. N., Mantis, A. J. ve Trakatellis, A. G. (1994). Rapid, Sensitive, and Specific Thiobarbituric Acid Method for Measuring Lipid Peroxidation in Animal Tissue, Food, and Feedstuff Samples. Journal of Agricultural and Food Chemistry, 42(9), 1931–1937. https://doi.org/10.1021/jf00045a019
- Budohoski, L., Panczenko-Kresowska, B., Langfort, J., Zernicka, E., Dubaniewicz, A., Ziemlanski, S., Challiss, R. A. J. ve Newsholme, E. A. (1993). Effects of saturated and polyunsaturated fat enriched diet on the skeletal muscle insulin sensitivity in young rats. Journal of Physiology and Pharmacology, 44(4), 391–398.
- Buettner, R. (2006). Defining high-fat-diet rat models: metabolic and molecular effects of different fat types. Journal of Molecular Endocrinology, 36(3), 485–501. https://doi.org/10.1677/jme.1.01909
- Buettner, R., Schölmerich, J. ve Bollheimer, L. C. (2007). High-fat Diets: Modeling the Metabolic Disorders of Human Obesity in Rodents*. Obesity, 15(4), 798–808. https://doi.org/10.1038/oby.2007.608
- Donohoue, P. A. (2004). Obesity. In: Behrman, R. E., Kliegman, R. M., Jenson, H. B., eds. Nelson Textbook of Pediatrics 17th ed. Philadelphia: W. B. Saunders, 173-177.
- Grundy, S. M. (2005). A constellation of complications: The metabolic syndrome. Clinical Cornerstone, 7(2-3), 36–45. https://doi.org/10.1016/s1098-3597(05)80066-3
- Harris, R. B. S. ve Kor, H. (1992). Insulin Insensitivity Is Rapidly Reversed in Rats by Reducing Dietary Fat from 40 to 30% of Energy. The Journal of Nutrition, 122(9), 1811–1822. https://doi.org/10.1093/jn/122.9.1811
