Piliç Eti ve Sakatat Örneklerinden İzole Edilen Bacillus cereus Suşlarında Antimikrobiyel Direncin Araştırılması

Aykut Arslan; H. Yeşim Can

TR EN

Piliç Eti ve Sakatat Örneklerinden İzole Edilen Bacillus cereus Suşlarında Antimikrobiyel Direncin Araştırılması

Öz

Bacillus cereus, doğada yaygın olarak bulunan, emetik ve diyarel formda gıda zehirlenmesine sebep olan patojen bir bakteridir. Bu çalışmada çiğ piliç eti ve sakatat örneklerinde B. cereus varlığı ile elde edilen izolatlarda disk difüzyon yöntemiyle beş farklı antibiyotiğe karşı direnç özelliği belirlenmiştir. Bu amaçla 30 adet piliç baget, 30 adet piliç kanat, 30 adet piliç göğüs ve 30 adet piliç sakatat olmak üzere, toplam 120 adet örnek B. cereus varlığı ve antimikrobiyel dirençliliği yönünden incelendi. Çalışma sonucunda 6’sı piliç baget, 8’i piliç kanat, 10’u piliç göğüs ve 12’si piliç sakatat örneğinde olmak üzere, toplam 120 örneğin 36 (%30)’sının B. cereus ile kontamine olduğu tespit edildi ve sonuçlar Real Time-PCR yöntemi ile doğrulandı. Doğrulanan 36 izolatta disk difüzyon testi ile 5 farklı antibiyotik (penisilin, eritromisin, tetrasiklin, kloramfenikol ve gentamisin) baz alınarak antibiyotik dirençliliği araştırıldı. İzolatların tamamının (%100) penisilin ve eritromisine karşı dirençli olduğu tespit edildi. İzolatlarda tetrasiklin, kloramfenikol ve gentamisine karşı dirençlilik sırasıyla %63,9, %41,7 ve %5,6 düzeyinde bulundu. Çoklu antibiyotik direnci izolatların %80,6’sında gözlendi. Kanatlı eti ve ürünlerinin B. cereus ile kontaminasyon düzeyini azaltmak için, kanatlı hayvanların yetiştirildiği kümes ortamından başlamak üzere, kesime kadar olan zincirde ve kesim sonrası aşamalarda gerekli hijyenik önlemler HACCP planı çerçevesinde yapılmalıdır. Ayrıca, antibiyotik dirençliliğinin önüne geçmek amacıyla kanatlılardaki antibiyotik kullanımı esaslarına dikkat edilmelidir.

Anahtar Kelimeler

Bacillus cereus , RT-PCR , Piliç eti , Sakatat , Antimikrobiyel direnç

Investigation of Antimicrobial Resistance in Bacillus cereus Strains Isolated from Broiler Meat and Offal Samples

Abstract

Bacillus cereus is a pathogenic bacterium commonly found in nature that causes emetic and diarrheal food poisoning. In this study, it was aimed to determine the presence of B. cereus in raw chicken meat and offal samples and the resistance properties of the isolates against five different antibiotics using disc diffusion method. In this study, a total of 120 food samples including 30 broiler drumstick, 30 broiler wing, 30 broiler breast, and 30 broiler offal samples were analyzed for the presence of B. cereus and its antimicrobial resistance. As a result of the study, 36 (30%) of a total of 120 food samples including 6 chicken drumsticks, 8 chicken wings, 10 chicken breasts, and 12 chicken offal samples, were found to be contaminated with B. cereus. The results were also confirmed by using the Real-Time PCR method. Antibiotic resistance in the 36 B. cereus isolates was investigated using the disc diffusion method against five different antibiotics: penicillin, erythromycin, tetracycline, chloramphenicol, and gentamicin. All isolates (100%) were found to be resistant to penicillin and erythromycin. Resistance to tetracycline, chloramphenicol, and gentamicin was determined as 63.9%, 41.7%, and 5.6%, respectively. Multidrug resistance was observed in 80.6% of the isolates. To reduce the contamination level with B. cereus in poultry meat and products, appropriate hygienic measures should be implemented within the HACCP framework starting from the poultry farm through to slaughter, and in post-slaughter stages. Furthermore, prudent use of antibiotics in poultry should be ensured to prevent the development of antimicrobial resistance.

Keywords

Bacillus cereus , RT-PCR , Broiler meat , Offal , Antimicrobial resistance

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Veteriner Bilimleri (Diğer)

Bölüm

Tez Özeti

Yazarlar

Aykut Arslan Bu kişi benim
0000-0001-8542-6614
Türkiye

H. Yeşim Can ^*
0000-0002-5191-6268
Türkiye

Yayımlanma Tarihi

31 Mart 2026

Gönderilme Tarihi

11 Şubat 2026

Kabul Tarihi

30 Mart 2026

Yayımlandığı Sayı

Yıl 1970 Sayı: 11

IZ

https://izlik.org/JA72XF86HZ

APA

Arslan, A., & Can, H. Y. (2026). Piliç Eti ve Sakatat Örneklerinden İzole Edilen Bacillus cereus Suşlarında Antimikrobiyel Direncin Araştırılması. Akademik Et ve Süt Kurumu Dergisi, 11, 39-49. https://izlik.org/JA72XF86HZ

Bu eser Creative Commons Atıf-GayriTicari 4.0 Uluslararası Lisansı ile lisanslanmıştır.

Piliç Eti ve Sakatat Örneklerinden İzole Edilen Bacillus cereus Suşlarında Antimikrobiyel Direncin Araştırılması

Öz

Anahtar Kelimeler

Investigation of Antimicrobial Resistance in Bacillus cereus Strains Isolated from Broiler Meat and Offal Samples

Abstract

Keywords

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

IZ

Kaynak Göster