Gökkuşağı Alabalığı (Oncorhynchus mykiss) Anaçlarında Açlık Döngüsü ve Yeniden Beslemenin Gamet Kalitesi ve Döllenme Üzerine Etkisi

Yıl 2024, Cilt: 55 Sayı: 3, 142 - 150, 29.09.2024

Nijat Nazarli , Güneş Yamaner

https://doi.org/10.17097/agricultureatauni.1457055

Öz

Bu çalışmada, açlık ve yeniden besleme rejimi uygulanan anaçlarda, bu besleme düzeninin gamet kalitesi ve döllenme üzerindeki etkisi incelenmiştir. Kontrol grubu balıkları her gün beslenirken, deneme grubunun erkek ve dişi bireyleri bir hafta beslenmiş, bir hafta aç bırakılmıştır. Ağırlık artışı en yüksek kontrol grubunda görülmüşken; yemden yararlanma oranı tüm gruplarda normal düzeyde bulunmuştur (%0,9-1,3). Relatif fekondite kontrol grubu dişilerinde 935±62 yumurta/kg; bir hafta açlık rejimi uygulanan balıklarda ise 1317±241 yumurta/kg olarak bulunmuştur (p < ,05). En düşük yumurta çapı bir hafta aç bırakılan grupta elde edilmiştir (3,36±0,2 mm). Sperm hacmi dışında tüm spermatolojik parametreler gruplar arasında benzerlik göstermiştir. Sperm miktarı bir hafta açlık uygulanan balıklarda (46,9±20 ml) kontrol grubuna nazaran daha yüksek bulunmuştur. Erkek bireylere dayalı döllenme çalışmasında kontrol dişi ile yapılan döllenme sonuçları deneme grubunun erkek bireyleri için benzerlik göstermiş, en yüksek döllenme oranı kontrol dişisi ve kontrol erkeği kullanılarak yapılan döllenmede görülmüştür.

Anahtar Kelimeler

Damızlık balık besleme, Yumurta kalitesi, Üreme performansı

Etik Beyan

Bu çalışma, hayvan deneyi İstanbul Üniversitesi Yerel Etik Kurulu tarafından onaylandıktan sonra gerçekleştirildi (Karar numarası: 2022/15).

Destekleyen Kurum

Bu çalışma İstanbul Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü tarafından desteklenmiştir [Proje numarası: FYL-2022-38913].

Proje Numarası

FYL-2022-38913

Teşekkür

Bu çalışma, İstanbul Üniversitesi Fen Bilimleri Enstitüsü'nde 2022 yılında tamamlanan yüksek lisans tezinden türetilmiştir. Yazarlar, desteklerinden dolayı İstanbul Üniversitesi Su Bilimleri Fakültesi Sapanca İç Sular Araştırma Merkezi yönetimine ve çalışanlarına içten şükranlarını sunar. Yazarlar ayrıca Doç. Dr. Aygül EKİCİ, Dr. Özgür ÇANAK, Dr. Rahmi Can ÖZDEMİR, doktora öğrencileri A. Mohammed Hassan AHMED, Muhammed Hanif AZHAR ve yüksek lisans öğrencileri K. Saheed OLUWASOLA ve J. Antonio ASPILCUETA VASQUEZ 'e içten teşekkürlerini sunarlar.

Effect of Starvation and Refeeding on Gamete Quality and Fertilization in Rainbow Trout (Oncorhynchus mykiss) Broodstock

Öz

In this study, the impact of starvation and refeeding on broodstocks was observed by examining quality parameters of gametes and fertilization. While the control group fish were fed every day, the male and female members of the group were fed one week apart and placed in starvation after a week of feeding. Weight gain in the control group was the highest. The feed conversion ratio was normal in all groups (0.9-1.3%). Relative fecundity was (935±62 eggs/kg) in control group females; it was found to be (1317±241 eggs/kg) in starving females and statistically different in the groups (p < .05). The egg diameter of the group receiving intermittent feeding for a week (3.36±0.2 mm) was found to be the lowest. All spermatological parameters were similar between groups, except for sperm volume. The most sperm count was seen in the group that received one-week intermittent feeding (46.9± 20 ml). The results of fertilization with the control female in the fertilization study based on male individuals showed similarity for the male individuals of the trial group, the highest fertilization rate was seen in fertilization using the control female and control male.

Anahtar Kelimeler

Broodfish feeding, Egg quality, Reproductive performance

Etik Beyan

This study was carried out after the animal experiment was approved by İstanbul University Local Ethics Committee (Decision number: 2022/15).

Destekleyen Kurum

This work was supported by Scientific Research Projects Coordination Unit of Istanbul University [Project number: FYL-2022-38913].

Proje Numarası

FYL-2022-38913

Teşekkür

This study was derived from a Master's thesis completed at Istanbul University, Institute of Graduate Studies in Sciences in 2022. The authors express their sincere gratitude to the management and staff of Sapanca Inland Waters Research Center of the Faculty of Aquatic Sciences, Istanbul University, Turkey for their support for their support. In addition, the authors would like to express their sincere thanks to Associate Professor Aygül EKİCİ, Dr. Özgür ÇANAK, Dr. Rahmi Can ÖZDEMİR, PhD student A. Mohammed Hassan AHMED; Muhammed Hanif AZHAR, and M.Sc. students K. Saheed OLUWASOLA and J. Antonio ASPILCUETA VASQUEZ.

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