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Enrichment of Bread with Whey Treated with High Hydrostatic Pressure

Yıl 2025, Cilt: 56 Sayı: 2, 102 - 111, 29.05.2025
https://doi.org/10.17097/agricultureatauni.1500607

Öz

There are some deficiencies in the nutritional profile of bread due to the use of refined flour in modern production. Therefore, various additives are employed to enhance the nutritional profile of bread. Whey is a valuable source of functional proteins, lipids, vitamins, and minerals. However, bread volume and water absorption decrease when whey is added directly to the bread dough. Therefore, studies are being carried out to investigate pretreatments that can improve the technological properties of whey. This study aimed to develop a bread product fortified with whey treated with high hydrostatic pressure (HHP) and evaluate its quality. Properties of bread enriched with whey treated with HHP (300 MPa, 3 min, 20°C) (HHPWB) were examined using crumb images, experimental (moisture, ash, and unsalted ash content, acidity, weight, dough yield, volume, specific volume, and crust/crumb ratio), texture, color, and sensory analyses. The HHPWB formulation resulted in a decrease in gas cells, hardness, and chewiness, as well as an increase in ash content, acidity, weight, volume, and a* color values. HHPWB received better sensory acceptance for crust, symmetry, and color. The HHP treatment may have the potential to boost the benefits of fortifying bread with whey.

Etik Beyan

None.

Destekleyen Kurum

Atatürk University

Proje Numarası

PRJ2010/257

Teşekkür

We would like to thank the Food Engineering Departments of Middle East Technical University, Hacettepe University and Ankara Halk Ekmek Un Fabrikası A.Ş. for laboratory facilities. In addition, we would like to express our gratitude to special thanks to Prof. Dr. Hami ALPAS for his assistance with HHP treatment and to special thanks to Prof. Dr. Ömer Cevdet BİLGİN for his assistance with the statistical analysis of the data.

Kaynakça

  • AACC (Approved methods of the American association of cereal chemists) (2000). American Association of Cereal Chemists. Approved Methods Committee, 1.
  • Adamczyk, G., Ivanišová, E., Kaszuba, J., Bobel, I., Khvostenko, K., Chmiel, M., & Falendysh, N. (2021). Quality assessment of wheat bread ıncorporating chia seeds. Foods, 10(10), 2376.
  • Aganovic, K., Hertel, C., Vogel, R. F., Johne, R., Schlüter, O., Schwarzenbolz, U., Jäger, H., Holzhauser, T., Bergmair, J., Roth, A., Sevenich, R., Bandick, N., Kulling, S. E., Knorr, D., Engel, K. H., & Heinz, V. (2021). Aspects of high hydrostatic pressure food processing: Perspectives on technology and food safety. Comprehensive Reviews in Food Science and Food Safety, 20(4), 3225-3266.
  • Alsaed, A. K., Ahmad, R., Aldoomy, H., El-Qader, S. A., Saleh, D., Sakejha, H., & Mustafa, L. (2013). Characterization, concentration and utilization of sweet and acid whey. Pakistan Journal of Nutrition, 12(2), 172-177.
  • Ambrosi, V., Polenta, G., Gonzalez, C., Ferrari, G., & Maresca, P. (2016). High hydrostatic pressure assisted enzymatic hydrolysis of whey proteins. Innovative Food Science and Emerging Technologies, 38, 294-301.
  • Basman, A., Köksel, H., & Ng, P. K. (2002). Effects of increasing levels of transglutaminase on the rheological properties and bread quality characteristics of two wheat flours. European Food Research and Technology, 215(5), 419-424.
  • Betoret, E., & Rosell, C. M. (2020). Enrichment of bread with fruits and vegetables: Trends and strategies to increase functionality. Cereal Chemistry, 97(1), 9-19.
  • Bilgiçli, N., & İbanoğlu, Ş. (2015). Effect of pseudo cereal flours on some physical, chemical and sensory properties of bread. Journal of Food Science and Technology, 52(11), 7525-7529.
  • Carullo, D., Barbosa-Cánovas, G. V., & Ferrari, G. (2021). Changes of structural and techno-functional properties of high hydrostatic pressure (HHP) treated whey protein isolate over refrigerated storage. LWT- Food Science and Technology, 137, 110436.
  • Chawla, R., Patil, G. R., & Singh, A. K. (2011). High hydrostatic pressure technology in dairy processing: A review. Journal of Food Science and Technology, 48(3), 260-268.
  • Chudy, S., Bilska, A., Kowalski, R., & Teichert, J. (2020). Colour of milk and milk products in CIE Lab space. Medycyna Weterynaryjna, 76(2), 77-81.
  • Çetiner, B., Acar, O., Kahraman, K., Sanal, T., & Koksel, H. (2017). An investigation on the effect of heat-moisture treatment on baking quality of wheat by using response surface methodology. Journal of Cereal Science, 74, 103-111.
  • Çiçek, S. (2011). The effect of addition of whey with high hidrostatic pressure microfluidizer and ultrasoundapplication on some quality parameters, colour, texture of the bread. (Master’s thesis). Atatürk Üniversity, Erzurum.
  • Das, B., Sarkar, S., Sarkar, A., Bhattacharjee, S., & Bhattacharjee, C. (2016). Recovery of whey proteins and lactose from dairy waste: A step towards green waste management. Process Safety and Environmental Protection, 101, 27-33.
  • Dıblan, S., Salum, P., Ulusal, F., & Erbay, Z. (2024). Impact of conjugation of whey protein concentrate with different carbohydrates: Monitoring structural and technofunctional variations. International Dairy Journal, 158, 106036.
  • Dinçoğlu, A. H., & Ardıç, M. (2012). Peynir altı suyunun beslenmemizdeki önemi ve kullanım olanakları. Harran Üniversitesi Veteriner Fakültesi Dergisi, 1(1), 54-60.
  • Divya, N., & Rao, K. J. (2010). Studies on utilization of indian cottage cheese whey in wheat bread manufacture. Journal of Food Processing and Preservation, 34(6), 975-992.
  • Elgün, A., Ertugay, Z., Certel, M., & Kotancılar, G. (1998). Analytical quality control and laboratory application guide in cereal and cereal products (in Turkish). Atatürk Uni Ziraat Fak Yay.
  • Erkan, N., Üretener, G., Alpas, H., Selçuk, A., Özden, O. & Buzrul, S. (2011). The effect of different high pressure conditions on the quality and shelf life of cold smoked fish. Innovative Food Science and Emerging Technologies, 12(2), 104-110.
  • Falade, E. O., Mu, T. H., & Zhang, M. (2021). Improvement of ultrasound microwave-assisted enzymatic production and high hydrostatic pressure on emulsifying, rheological and interfacial characteristics of sweet potato protein hydrolysates. Food Hydrocolloids, 117, 106684.
  • Feng, J., Berton-Carabin, C. C., Fogliano, V., & Schroën, K. (2022). Maillard reaction products as functional components in oil-in-water emulsions: A review highlighting interfacial and antioxidant properties. Trends in Food Science & Technology, 121, 129-141.
  • Ferreyra, L. S., Verdini, R. A., Soazo, M., & Piccirilli, G. N. (2021). Impact of whey protein addition on wheat bread fermented with a spontaneous sourdough. International Journal of Food Science and Technology, 56(9), 4738-4745.
  • Gernigon, G., Schuck, P., & Jeantet, R. (2010). Processing of Mozzarella cheese wheys and stretchwaters: A preliminary review. Dairy Science and Technology, 90(1), 27-46.
  • Guiné, R. P. F., Santos, C., Rocha, C., Marques, C., Rodrigues, C., Manita, F., Sousa, F., Félix, M., Silva, S., & Rodrigues, S. (2020). Whey-bread, an improved food product: Evaluation of textural characteristics. Journal of Culinary Science and Technology, 18(1), 40-53.
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  • Ingrassia, R., Torres, P., Bojanich, L., Ratti, J., Baldor, S., Ramunno, C., Dotta, G., Vidal Tesón, A., Forastieri, P., Soazo, M., Spelzini, D., Narambuena, C., & Boeris, V. (2022). Concentration of proteins and fat from whey by coacervation: Evaluation of its incorporation in bread. Journal of Food Processing and Preservation, 46(2).
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Ekmeğin Yüksek Hidrostatik Basınçla İşlenmiş Peynir Altı Suyuyla Zenginleştirilmesi

Yıl 2025, Cilt: 56 Sayı: 2, 102 - 111, 29.05.2025
https://doi.org/10.17097/agricultureatauni.1500607

Öz

Modern üretimde rafine un kullanılması nedeniyle ekmeğin besin profilinde bazı eksiklikler bulunmaktadır. Bu nedenle, ekmeğin besin profilini geliştirmek için çeşitli katkı maddeleri kullanılmaktadır. Peynir altı suyu fonksiyonel proteinler, lipitler, vitaminler ve mineraller açısından değerli bir kaynaktır. Ancak peynir altı suyu doğrudan ekmek hamuruna eklendiğinde ekmek hacmi ve su emilimi azalmaktadır. Bu nedenle, peynir altı suyunun teknolojik özelliklerini geliştirebilecek ön işlemler ile ilgili araştırmak için çalışmalar yürütülmektedir. Bu çalışma, yüksek hidrostatik basınç (HHP) ile işlenmiş peynir altı suyuyla zenginleştirilmiş bir ekmek ürünü geliştirmeyi ve kalitesini değerlendirmeyi amaçlamıştır. HHP (300 MPa, 3 dakika, 20°C) ile işlenmiş peynir altı suyuyla zenginleştirilmiş ekmeğin (HHPWB) özellikleri, kırıntı görüntüleri, deneysel (nem, kül ve tuzsuz kül içeriği, asitlik, ağırlık, hamur verimi, hacim, özgül hacim ve kabuk/iç oranı), doku, renk ve duyusal analizler kullanılarak incelenmiştir. HHPWB formülasyonu gaz hücrelerinde, sertlikte ve çiğnenebilirlikte azalmanın yanı sıra kül içeriği, asitlik, ağırlık, hacim ve a* renk değerlerinde artışa neden olmuştur. HHPWB kabuk, simetri ve renk açısından daha iyi duyusal kabul görmüştür. HHP uygulaması ekmeğin peynir altı suyuyla zenginleştirilmesinin faydalarını artırma potansiyeline sahip olabilir.

Etik Beyan

Yok.

Destekleyen Kurum

Atatürk Üniversitesi

Proje Numarası

PRJ2010/257

Teşekkür

Orta Doğu Teknik Üniversitesi, Hacettepe Üniversitesi ve Ankara Halk Ekmek Un Fabrikası A.Ş.'nin Gıda Mühendisliği Bölümlerine teşekkür ederiz. laboratuvar tesisleri için. Ayrıca HHP tedavisindeki yardımlarından dolayı Prof. Dr. Hami ALPAS'a, verilerin istatistiksel analizindeki yardımlarından dolayı Prof. Dr. Ömer Cevdet BİLGİN'e özel teşekkürlerimizi sunarız.

Kaynakça

  • AACC (Approved methods of the American association of cereal chemists) (2000). American Association of Cereal Chemists. Approved Methods Committee, 1.
  • Adamczyk, G., Ivanišová, E., Kaszuba, J., Bobel, I., Khvostenko, K., Chmiel, M., & Falendysh, N. (2021). Quality assessment of wheat bread ıncorporating chia seeds. Foods, 10(10), 2376.
  • Aganovic, K., Hertel, C., Vogel, R. F., Johne, R., Schlüter, O., Schwarzenbolz, U., Jäger, H., Holzhauser, T., Bergmair, J., Roth, A., Sevenich, R., Bandick, N., Kulling, S. E., Knorr, D., Engel, K. H., & Heinz, V. (2021). Aspects of high hydrostatic pressure food processing: Perspectives on technology and food safety. Comprehensive Reviews in Food Science and Food Safety, 20(4), 3225-3266.
  • Alsaed, A. K., Ahmad, R., Aldoomy, H., El-Qader, S. A., Saleh, D., Sakejha, H., & Mustafa, L. (2013). Characterization, concentration and utilization of sweet and acid whey. Pakistan Journal of Nutrition, 12(2), 172-177.
  • Ambrosi, V., Polenta, G., Gonzalez, C., Ferrari, G., & Maresca, P. (2016). High hydrostatic pressure assisted enzymatic hydrolysis of whey proteins. Innovative Food Science and Emerging Technologies, 38, 294-301.
  • Basman, A., Köksel, H., & Ng, P. K. (2002). Effects of increasing levels of transglutaminase on the rheological properties and bread quality characteristics of two wheat flours. European Food Research and Technology, 215(5), 419-424.
  • Betoret, E., & Rosell, C. M. (2020). Enrichment of bread with fruits and vegetables: Trends and strategies to increase functionality. Cereal Chemistry, 97(1), 9-19.
  • Bilgiçli, N., & İbanoğlu, Ş. (2015). Effect of pseudo cereal flours on some physical, chemical and sensory properties of bread. Journal of Food Science and Technology, 52(11), 7525-7529.
  • Carullo, D., Barbosa-Cánovas, G. V., & Ferrari, G. (2021). Changes of structural and techno-functional properties of high hydrostatic pressure (HHP) treated whey protein isolate over refrigerated storage. LWT- Food Science and Technology, 137, 110436.
  • Chawla, R., Patil, G. R., & Singh, A. K. (2011). High hydrostatic pressure technology in dairy processing: A review. Journal of Food Science and Technology, 48(3), 260-268.
  • Chudy, S., Bilska, A., Kowalski, R., & Teichert, J. (2020). Colour of milk and milk products in CIE Lab space. Medycyna Weterynaryjna, 76(2), 77-81.
  • Çetiner, B., Acar, O., Kahraman, K., Sanal, T., & Koksel, H. (2017). An investigation on the effect of heat-moisture treatment on baking quality of wheat by using response surface methodology. Journal of Cereal Science, 74, 103-111.
  • Çiçek, S. (2011). The effect of addition of whey with high hidrostatic pressure microfluidizer and ultrasoundapplication on some quality parameters, colour, texture of the bread. (Master’s thesis). Atatürk Üniversity, Erzurum.
  • Das, B., Sarkar, S., Sarkar, A., Bhattacharjee, S., & Bhattacharjee, C. (2016). Recovery of whey proteins and lactose from dairy waste: A step towards green waste management. Process Safety and Environmental Protection, 101, 27-33.
  • Dıblan, S., Salum, P., Ulusal, F., & Erbay, Z. (2024). Impact of conjugation of whey protein concentrate with different carbohydrates: Monitoring structural and technofunctional variations. International Dairy Journal, 158, 106036.
  • Dinçoğlu, A. H., & Ardıç, M. (2012). Peynir altı suyunun beslenmemizdeki önemi ve kullanım olanakları. Harran Üniversitesi Veteriner Fakültesi Dergisi, 1(1), 54-60.
  • Divya, N., & Rao, K. J. (2010). Studies on utilization of indian cottage cheese whey in wheat bread manufacture. Journal of Food Processing and Preservation, 34(6), 975-992.
  • Elgün, A., Ertugay, Z., Certel, M., & Kotancılar, G. (1998). Analytical quality control and laboratory application guide in cereal and cereal products (in Turkish). Atatürk Uni Ziraat Fak Yay.
  • Erkan, N., Üretener, G., Alpas, H., Selçuk, A., Özden, O. & Buzrul, S. (2011). The effect of different high pressure conditions on the quality and shelf life of cold smoked fish. Innovative Food Science and Emerging Technologies, 12(2), 104-110.
  • Falade, E. O., Mu, T. H., & Zhang, M. (2021). Improvement of ultrasound microwave-assisted enzymatic production and high hydrostatic pressure on emulsifying, rheological and interfacial characteristics of sweet potato protein hydrolysates. Food Hydrocolloids, 117, 106684.
  • Feng, J., Berton-Carabin, C. C., Fogliano, V., & Schroën, K. (2022). Maillard reaction products as functional components in oil-in-water emulsions: A review highlighting interfacial and antioxidant properties. Trends in Food Science & Technology, 121, 129-141.
  • Ferreyra, L. S., Verdini, R. A., Soazo, M., & Piccirilli, G. N. (2021). Impact of whey protein addition on wheat bread fermented with a spontaneous sourdough. International Journal of Food Science and Technology, 56(9), 4738-4745.
  • Gernigon, G., Schuck, P., & Jeantet, R. (2010). Processing of Mozzarella cheese wheys and stretchwaters: A preliminary review. Dairy Science and Technology, 90(1), 27-46.
  • Guiné, R. P. F., Santos, C., Rocha, C., Marques, C., Rodrigues, C., Manita, F., Sousa, F., Félix, M., Silva, S., & Rodrigues, S. (2020). Whey-bread, an improved food product: Evaluation of textural characteristics. Journal of Culinary Science and Technology, 18(1), 40-53.
  • Hossein, J. (2009). Evaluation of physical and sensory properties of Iranian Lavash flat bread supplemented with precipitated whey protein (PWP). African Journal of Food Science, 3(2), 28-34.
  • Huang, H. W., Hsu, C. P., & Wang, C. Y. (2020). Healthy expectations of high hydrostatic pressure treatment in food processing industry. Journal of Food and Drug Analysis, 28(1), 1-13.
  • Ingrassia, R., Torres, P., Bojanich, L., Ratti, J., Baldor, S., Ramunno, C., Dotta, G., Vidal Tesón, A., Forastieri, P., Soazo, M., Spelzini, D., Narambuena, C., & Boeris, V. (2022). Concentration of proteins and fat from whey by coacervation: Evaluation of its incorporation in bread. Journal of Food Processing and Preservation, 46(2).
  • Iuga, M., Boestean, O., Ghendov-Mosanu, A., & Mironeasa, S. (2020). Impact of dairy ingredients on wheat flour dough rheology and bread properties. Foods, 9(6), 828.
  • Kadharmestan, C., Baik, B. K., & Czuchajowska, Z. (1998). Whey protein concentrate treated with heat or high hydrostatic pressure in wheat-based products. Cereal Chemistry, 75(5), 762-766.
  • Kittisuban, P., Ritthiruangdej, P., & Suphantharika, M. (2014). Optimization of hydroxypropylmethylcellulose, yeast β-glucan, and whey protein levels based on physical properties of gluten-free rice bread using response surface methodology. LWT - Food Science and Technology, 57(2), 738-748.
  • Komeroski, M. R., & Oliveira, V. R. D. (2023). Influence of the amount and type of whey protein on the chemical, technological, and sensory quality of pasta and bakery products. Foods, 12(14), 2801-2016.
  • Kourkouta, L., Koukourikos, K., Iliadis, C., Ouzounakis, P., Monios, A., Tsaloglidou, & A. (2017). Bread and health. Journal of Pharmacy and Pharmacology, 5(11).
  • Kurt, A., Cakmakci, S., & Caglar, A. (1996). A guide book of analysis methods of milk and milk products. In Agricultural Faculty Pub. No 18.
  • Lachowicz, S., Świeca, M., & Pejcz, E. (2021). Biological activity, phytochemical parameters, and potential bioaccessibility of wheat bread enriched with powder and microcapsules made from Saskatoon berry. Food Chemistry, 338, 128026.
  • Lee, W., Clark, S., & Swanson, B. G. (2006). Functional properties of high hydrostatic pressure-treated whey protein. Journal of Food Processing and Preservation, 30(4), 488-501.
  • Lim, S. Y., Swanson, B. G., & Clark, S. (2008a). High hydrostatic pressure modification of whey protein concentrate for improved functional properties. Journal of Dairy Science, 91(4), 1299-1307.
  • Lim, S. Y., Swanson, B. G., Ross, C. F., & Clark, S. (2008b). High hydrostatic pressure modification of whey protein concentrate for improved body and texture of lowfat ice cream. Journal of Dairy Science, 91(4), 1308-1316.
  • Liu, X., Powers, J. R., Swanson, B. G., Hill, H. H., & Clark, S. (2005). High hydrostatic pressure affects flavor-binding properties of whey protein concentrate. Journal of Food Science, 70(9), 581-585.
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  • López-fandiño, R. (2006). Functional improvement of milk whey proteins induced by high hydrostatic pressure. Critical Reviews in Food Science and Nutrition, 46(4), 351-363.
  • Madenci, A. B., & Bilgiçli, N. (2014). Effect of whey protein concentrate and buttermilk powders on rheological properties of dough and bread quality. Journal of Food Quality, 37(2), 117-124.
  • Martins, Z. E., Pinho, O., & Ferreira, I. M. P. L. V. O. (2017). Food industry by-products used as functional ingredients of bakery products. Trends in Food Science and Technology, 67, 106-128.
  • Meral, R., & Erim Köse, Y. (2019). The effect of bread-making process on the antioxidant activity and phenolic profile of enriched breads. Quality Assurance and Safety of Crops and Foods, 11(2), 171-181.
  • Mzoughi, M., Demircan, E., Zouari, A., & Toker, O. S. (2024). Maillard Reaction for Protein Fortification in Bakery Products. In Fortified Foods (pp. 263-304). New York, NY: Springer US.
  • Nwosu, J. N., Owuamanam, C. I., Omeire, G. C., & Eke, C. C. (2014). Quality parameters of bread produced from substitution of wheat flour with cassava flour using soybean as an improver. American Journal of Research Communication, 2(3), 99-118.
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  • Wang, C. Y., Huang, H. W., Hsu, C. P., & Yang, B. B. (2016). Recent advances in food processing using high hydrostatic pressure technology. Critical Reviews in Food Science and Nutrition, 56(4), 527-540.
  • Wang, W. Q., Bao, Y. H., & Chen, Y. (2013). Characteristics and antioxidant activity of water-soluble Maillard reaction products from interactions in a whey protein isolate and sugars system. Food Chemistry, 139(1–4), 355-361.
  • Xiang, J., Liu, F., Wang, B., Chen, L., Liu, W., & Tan, S. (2021). A literature review on maillard reaction based on milk proteins and carbohydrates in food and pharmaceutical products: advantages, disadvantages, and avoidance strategies. Foods, 10(9), 1998-2015.
  • Yadav, J. S. S., Yan, S., Pilli, S., Kumar, L., Tyagi, R. D., & Surampalli, R. Y. (2015). Cheese whey: A potential resource to transform into bioprotein, functional/nutritional proteins and bioactive peptides. Biotechnology Advances, 33(6), 756-774.
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  • Zandona, E., Blažić, M., & Režek Jambrak, A. (2021). Whey utilisation: Sustainable uses and environmental approach. Food Technology and Biotechnology, 59(2), 147-161.
  • Zhang, J., Tu, W., Shen, Y., Wang, H., Yang, J., Ma, M., Man, C., Zhang, W., Zhao, Q., & Jiang, Y. (2024). Changes in whey protein produced by different sterilization processes and lactose content: Effects on glycosylation degree and whey protein structure. Food Bioscience, 62, 105040.
  • Zhou, J., Liu, J., & Tang, X. (2018). Effects of whey and soy protein addition on bread rheological property of wheat flour. Journal of Texture Studies, 49(1), 38-46.
Toplam 62 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Ziraat Mühendisliği (Diğer)
Bölüm Araştırma Makalesi
Yazarlar

Semra Çiçek 0000-0002-2927-2793

Ferid Aydın 0000-0002-9931-6202

Faruk Bozoglu 0000-0002-1394-345X

Proje Numarası PRJ2010/257
Erken Görünüm Tarihi 28 Mayıs 2025
Yayımlanma Tarihi 29 Mayıs 2025
Gönderilme Tarihi 13 Haziran 2024
Kabul Tarihi 13 Şubat 2025
Yayımlandığı Sayı Yıl 2025 Cilt: 56 Sayı: 2

Kaynak Göster

APA Çiçek, S., Aydın, F., & Bozoglu, F. (2025). Enrichment of Bread with Whey Treated with High Hydrostatic Pressure. Research in Agricultural Sciences, 56(2), 102-111. https://doi.org/10.17097/agricultureatauni.1500607

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