Antifungal activity of Eugenia caryophyllata, Cinnamomum sp., Mentha piperita, and Thymus vulgaris essential oils against Aspergillus niger

Öz

Aspergillus species are pollutants found in both food and air. The increase in the metabolic activity of Aspergillus leads to spoilage of foodstuffs and large economic losses. In addition, some Aspergillus species have the ability to produce aflatoxins and ochratoxins, secondary metabolites called, namely, mycotoxins. Especially mycotoxins are very important in terms of food safety and human health. Since the protection of human and animal health and the prevention of economic losses is a very important issue, our study aimed to determine the antifungal activity of Eugenia caryophyllata Thunb., Cinnamomum sp., Mentha piperita L. and Thymus vulgaris L. essential oils (EO) against Aspergillus niger Tiegh. NRRL 321 strain. In the second step, MIC and MFC values of EOs were determined. It was determined that the MIC value of Cinnamomum sp, M. piperita and T. vulgaris EOs was 0.01 µL/mL, and E. caryophyllata EO was 0.5 µL/mL. It was determined that E. caryophyllata, Mentha piperita and Thymus vulgaris EOs completely inhibited radial colony growth at MIC, 2xMIC and 4xMIC values. It was determined that the inhibition of radial growth of Cinnamomum sp. EO varies depending on the concentration, and the inhibition rate increases as the concentration increases. As a result, evaluations should be made considering the in vivo conditions that the tested EOs showed strong antifungal activity against the A. niger strain.

Anahtar Kelimeler

• Antifungal activity
• cinnamon oil
• clove oil
• peppermint oil
• thyme oil

Proje Numarası

THD-2021-3571

Eugenia caryophyllata, Cinnamomum sp., Mentha piperita ve Thymus vulgaris esansiyel yağların Aspergillus niger suşuna karşı antifungal aktivitesi

Öz

Aspergillus türleri hem gıdalarda hem de havada bulunan kirleticilerdir. Aspergillus cinsinin metabolik aktivitesindeki artış, gıda maddelerinin bozulmasına ve büyük ekonomik kayıpların oluşmasına yol açmaktadır. Ayrıca bazı Aspergillus türleri, aflatoksinler, okratoksinler olarak adlandırılan sekonder metabolitleri yani mikotoksinleri üretme yeteneğine sahiptir. Özellikle mikotoksinler, gıda güvenliği ve insan sağlığı açısından oldukça önemlidir. İnsan ve hayvan sağlığının korunması ve ekonomik kayıpların önlenmesi çok önemli bir konu olması sebebiyle, çalışma amacımız Aspergillus niger Tiegh. NRRL 321 suşuna karşı Eugenia caryophyllata Thunb., Cinnamomum sp., Mentha piperita L. ve Thymus vulgaris L. esansiyel yağlarının (EO) antifungal aktivitesi belirlenmiştir. İkinci adımda ise EO’ların MİK ve MFK değerleri belirlendi. Cinnamomum sp., M. piperita ve T. vulgaris EO’ların MİK değerinin 0,01 µL/mL, E. caryophyllata EO’nun ise 0,5 µL/mL olduğu tespit edilmiştir. E. caryophyllata, M. piperita ve T. vulgaris EO’ların MİK, 2xMİK ve 4xMİK değerlerinde radyal koloni gelişimini tamamen inhibe ettiği belirlendi. Cinnamomum sp. EO’nun ise radyal büyümenin inhibisyonu konsantrasyona bağlı olarak değiştiği, konsantrasyon arttıkça inhibisyon oranın arttığı belirlenmiştir. Sonuç olarak test edilen EO’ların A. niger suşuna karşı güçlü antifungal aktivite gösterdiği, in vivo koşullar göz önüne alınarak değerlendirmelerin yapılması gerekmektedir.

Anahtar Kelimeler

• Antifungal aktivite
• karanfil yağı
• kekik yağı
• nane yağı
• tarçın yağı

Destekleyen Kurum

Çanakkale Onsekiz Mart Üniversitesi

Proje Numarası

THD-2021-3571

Kaynakça

1. Al-Shahrani MH, Mahfoud M, Anvarbatcha R, Athar MT, Al Asmari A (2017). Evaluation of antifungal activity and cytotoxicity of Thymus vulgaris essential oil. Pharmacognosy Communications 7(1): 34-40.
2. Burt S (2004). Essential oils: their antibacterial properties and potential applications in foods - a review. International journal of food microbiology 94(3): 223-253.
3. Desam NR, Al-Rajab AJ, Sharma M, Mylabathula MM, Gowkanapalli RR, Albratty M (2019). Chemical constituents, in vitro antibacterial and antifungal activity of Mentha × Piperita L.(peppermint) essential oils. Journal of King Saud University-Science 31(4): 528-533.
4. Fogarty WM (1994). Enzymes of the genus Aspergillus. In Smith JE (edt.). Aspergillus. Boston: Springer.
5. Gámez-Espinosa E, Anaya M, Borges P, Crespo DMB (2021). Antifungal effects of Curcuma longa L. essential oil against pathogenic strains isolated from indoor air. Aerobiologia 37: 119-126.
6. Gatsing D, Tchakoute V, Ngamga D, Kuiate JR, Tamokou JDD, Nji Nkah BF, Tchouanguep FM, Fodouop SPC (2009). In Vitro antibacterial activity of Crinum purpurascens herb leaf extract against the Salmonella species causing typhoid fever and its toxicological evaluation. Iranian Journal of Medical Sciences 34(2): 126-136.
7. Hu F, Tu XF, Thakur K, Hu F, Li XL, Zhang YS, Zhang JG, Wei ZJ (2019). Comparison of antifungal activity of essential oils from different plants against three fungi. Food and Chemical Toxicology 134: 110821.
8. Hua H, Xing F, Selvaraj JN, Wang Y, Zhao Y, Zhou L, Li X, Liu Y (2014). Inhibitory effect of essential oils on Aspergillus ochraceus growth and ochratoxin A production. PloS one 9(9): e108285.

9. Klich MA (2002). Biogeography of Aspergillus species in soil and litter. Mycologia 94(1): 21-27.
10. Klich MA, Tang S, Denning DW (2009). Aflatoxin and ochratoxin production by Aspergillus species under ex vivo conditions. Mycopathologia 168(4): 185-191.
11. Kumar A, Shukla R, Singh P, Prasad CS, Dubey NK (2008). Assessment of Thymus vulgaris L. essential oil as a safe botanical preservative against post harvest fungal infestation of food commodities. Innovative Food Science & Emerging Technologies 9(4): 575-580.
12. Kumar P, Mishra S, Kumar A, Kumar S, Prasad CS (2017). In vivo and in vitro control activity of plant essential oils against three strains of Aspergillus niger. Environmental Science and Pollution Research 24(27): 21948-21959.
13. Lappa IK, Simini E, Nychas GJE, Panagou EZ (2017). In vitro evaluation of essential oils against Aspergillus carbonarius isolates and their effects on Ochratoxin A related gene expression in synthetic grape medium. Food Control 73: 71-80.
14. Mahboubi M, Kazempour N (2014). Chemical composition and antimicrobial activity of peppermint (Mentha piperita L.) essential oil. Songklanakarin Journal of Science and Technology 36(1): 83-87.
15. Moghadam ZA, Hosseini H, Hadian Z, Asgari B, Mirmoghtadaie L, Mohammadi A, Shamloo E, Javadi NHS. (2019). Evaluation of the antifungal activity of cinnamon, clove, thymes, zataria multiflora, cumin and caraway essential oils against Ochratoxigenic Aspergillus ochraceus. Journal of Pharmaceutical Research International 26(1): 1-16.
16. Mseddi K, Alimi F, Noumi E, Veettil VN, Deshpande S, Adnan M, Hamdi A, Elkahoui S, Alghamdi A, Kadri A, Patel M, Snoussi M (2020). Thymus musilii Velen. as a promising source of potent bioactive compounds with its pharmacological properties: In vitro and in silico analysis. Arabian Journal of Chemistry 13(8): 6782-6801.
17. Nedorostova L, Kloucek P, Kokoska L, Stolcova M, Pulkrabek J (2009). Antimicrobial properties of selected essential oils in vapour phase against foodborne bacteria. Food control 20(2): 157-160.
18. Pekmezovic M, Rajkovic K, Barac A, Senerović L, Arsenijevic VA (2015). Development of kinetic model for testing antifungal effect of Thymus vulgaris L. and Cinnamomum cassia L. essential oils on Aspergillus flavus spores and application for optimization of synergistic effect. Biochemical Engineering Journal 99: 131-137.
19. Prakash B, Kedia A, Mishra PK, Dubey NK (2015). Plant essential oils as food preservatives to control moulds, mycotoxin contamination and oxidative deterioration of agri-food commodities–Potentials and challenges. Food Control 47: 381-391.
20. Snoussi M, Noumi E, Punchappady-Devasya R, Trabelsi N, Kanekar S, Nazzaro F, Fratianni F, Flamini G, De Feo V, Al-Sieni A (2018). Antioxidant properties and anti-quorum sensing potential of Carum copticum essential oil and phenolics against Chromobacterium violaceum. Journal of Food Science and Technology 55(8): 2824-2832.
21. Soliman KM, Badeaa RI (2002). Effect of oil extracted from some medicinal plants on different mycotoxigenic fungi. Food and Chemical Toxicology 40(11): 1669-1675.
22. Thanaboripat D, Suvathi Y, Srilohasin P, Sripakdee S, Patthanawanitchai O, Charoensettasilp S (2007). Inhibitory effect of essential oils on the growth of Aspergillus flavus. Current Applied Science and Technology 7(1): 1-7.
23. Vijayalakshmi A, Sharmila R, Gowda NKS, Sindhu G. (2014). Study on antifungal effect of herbal compounds against mycotoxin producing fungi. International Journal of Agricultural Technology 10(6): 1587-1597.
24. Yan J, Niu Y, Wu C, Shi Z, Zhao P, Naik N, Mai X, Yuan B (2021). Antifungal effect of seven essential oils on bamboo. Advanced Composites and Hybrid Materials 4(3): 552-561.