The protective role of resveratrol against zinc oxide induced nanotoxicity

Öz

Zinc oxide (ZnO) is a compound that has harmful effects as well as being used in many different areas. Numerous studies have been carried out to minimize the toxic effects of ZnO nanoparticles (NPs). In the present study, the protective role of resveratrol (RSV), a potent antioxidant polyphenol substance, was examined against ZnO-induced nanotoxicity on human pulmonary alveolar epithelial cells (HPAEpiC). In this context, the cytotoxic and genotoxic effects of different concentrations of RSV (5, 10, 20 mg/L) and ZnO NPs on the cells were measured alone and in combination. At the same time, the effects of aforementioned applications on the total antioxidant capacity (TAC) level in HPAEpiC were assessed. The results obtained showed that ZnO NPs alone significantly increased cytotoxicity and genotoxicity on cells compared to negative control (control (-)). In the experiments performed with RSV + ZnO NP combination, cytotoxic and genotoxic activity decreased at the level of p < 0.05 especially at 20 mg/L application of RSV. When the level of TAC in cells was examined, a concentration-dependent increase was detected between TAC and RSV. It was determined that ZnO NPs reduced the TAC level statistically (p < 0.05) in comparison with control (-). In conclusion, the present study revealed that RSV, a natural antioxidant, showed protective property against genotoxic and cytotoxic damage induced by ZnO NPs on HPAEpiC.

Anahtar Kelimeler

• Antioxidant
• Nanotoxicity
• Resveratrol
• Zinc oxide

Resveratrolün çinko oksit indüklü nanotoksisiteye karşı koruyucu rolü

Öz

Çinko oksit (ZnO) birçok farklı alanda kullanılmakla beraber zararlı etkileri de olan bir bileşiktir. ZnO nanopartiküllerinin (NPler) gösterdiği toksik etkileri en az seviyeye indirmek için çok sayıda çalışma gerçekleştirilmektedir. Mevcut çalışmada, güçlü bir antioksidan polifenol madde olan resveratrolün (RSV) insan akciğer alveol epitel hücreleri (İAAEpiH) üzerinde ZnO indüklü nanotoksisiteye karşı koruyucu rolü incelenmiştir. Bu kapsamda, RSV’nin farklı konsantrasyonlarının (5, 10, 20 mg/L) ve ZnO NPler’in hücreler üzerindeki sitotoksik ve genotoksik etkileri tek başlarına ve kombine olarak ölçülmüştür. Aynı zamanda, bahsi geçen uygulamaların İAAEpiH’deki toplam antioksidan kapasite (TAK) düzeyine etkileri değerlendirilmiştir. Elde edilen sonuçlar, ZnO NPler’in tek başına hücreler üzerinde sitotoksisite ve genotoksisiteyi negatif kontrole (kontrol (-)) kıyasla önemli derecede yükselttiğini göstermiştir. RSV + ZnO NP kombineli gerçekleştirilen denemelerde, özellikle RSV’nin 20 mg/L’lik uygulamasında sitotoksik ve genotoksik aktivite p < 0.05 seviyesinde düşmüştür. Hücrelerdeki TAK seviyesi incelendiğinde, TAK ile RSV arasında konsantrasyona bağlı bir artış tespit edilmiştir. ZnO NPlerin ise TAK düzeyini kontrol (- )’ye kıyasla istatistiki (p < 0.05) açıdan yüksek derecede düşürdüğü belirlenmiştir. Sonuç olarak, mevcut çalışma, doğal bir antioksidan olan RSV’nin İAAEpiH’leri üzerinde ZnO NPleri tarafından indüklenen genotoksik ve sitotoksik hasara karşı koruyucu özellik gösterdiğini ortaya çıkarmıştır.

Anahtar Kelimeler

• Antioksidan
• Nanotoksisite
• Resveratrol
• Çinko oksit

Kaynakça

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