Koşullandırılmış Kalem Grafit Elektrot Kullanılarak Vanilinin Voltammetrik Tayini

Yıl 2019, , 1 - 8, 26.03.2019

Didem Giray Dilgin

https://doi.org/10.24323/akademik-gida.543981

Cited By: 3

Öz

Bu çalışmada, bir elektrot malzemesi olarak
kurşun kalem ucu kullanılarak ticari gıda ürünlerinde vanilin tayini için
hassas bir voltammetrik yöntem önerilmiştir. Vanilinin, önceden oksitlenmiş
grafit kalem elektrotu (pre-oxidized pencil graphite electrode, p-PGE)
kullanılarak pH 8.0 Britton Robinson tampon çözeltisinde kaydedilen döngüsel
voltammogramlarında, yaklaşık 520 mV’da (Ag/AgCl elektroduna karşı) vanilinin
yükseltgenmesine atfedilen keskin bir pik gözlenmiştir. Daha sonra, vanilinin
p-PGE’de yükseltgenmesine dayalı voltammetrik vanilin tayini, optimize koşullar
altında diferansiyel puls voltammetri (DPV) tekniği kullanılarak
gerçekleştirildi. DPV sonuçları, vanilinin yükseltgenmesine ait pik akımının, 0,16
µM (3S_b’ye dayanarak) gözlenebilme sınırı ile birlikte 0,5-10,0 µM vanilin
derişim aralığında doğrusal olarak arttığını göstermektedir. Çalışmanın son
aşamasında, bu voltammetrik yöntem vanilya şurubu örneklerinde vanilin tayinine
uygulanmıştır. Elde edilen sonuçların numune şişesindeki etiketli değerler ile
iyi bir uyum içinde olduğu tespit edilmiştir.

Anahtar Kelimeler

Kalem grafit elektrot, Vanilin, Voltammetrik tayin, Diferansiyel puls voltammetri

Voltammetric Determination of Vanillin Using a Pretreated Pencil Graphite Electrode

Öz

In this study, a sensitive voltammetric method
for the determination of vanillin in commercial food products was proposed
using a pencil lead as an electrode material. In the cyclic voltammograms of
vanillin recorded in pH 8.0 Britton Robinson buffer at the pre-oxidized pencil graphite
electrode (p-PGE), a sharp peak was observed at about 520 mV (vs. Ag / AgCl
electrode) attributed to the oxidation of vanillin. Subsequently, the
voltammetric determination of vanillin based on its oxidation at p-PGE was
carried out using the differential pulse voltammetry (DPV) technique under
optimized conditions. DPV results showed that the oxidation peak current of
vanillin increased linearly in the concentration range of 0.5 to 10.0 µM
vanillin with a detection limit of 0.16 µM (based on 3S_b). In the final
step, this voltammetric method was applied to the determination of vanillin in
vanilla syrup samples. Results were in good agreement with the values indicated
on the labels of samples.

Anahtar Kelimeler

Pencil graphite electrode, Vanillin, Voltammetric determination, Differential pulse voltammetry

Kaynakça

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