İğde (Elaeagnus angustifolia L.) Meyve ve Yapraklarının Antioksidan ve Antidiyabetik Özellikleri

Yıl 2020, , 270 - 278, 29.10.2020

Serap Berktaş Mustafa Çam

https://doi.org/10.24323/akademik-gida.818125

Cited By: 5

Öz

Bu çalışma Elaeagnus angustifolia L. (iğde) meyve ve yapraklarının antioksidan ve antidiyabetik etkilerini belirlemek ve karşılaştırmak için gerçekleştirilmiştir. Metanol:su (1:1, v/v) ile yapılan iğde meyve ve yaprak ekstraktlarının toplam fenolik, toplam flavonoid ve tanen içerikleri, antioksidan aktiviteleri (DPPH ve ABTS metotları) ve α-glukozidaz ve α-amilaz enzimlerini inhibe edici etkileri araştırılmıştır. Ayrıca ekstraktlarda lipaz inhibisyonu aktivitesi tayini de gerçekleştirilmiştir. İğde meyve ve yapraklarının α-glukozidaz enzimini %50 oranında inhibe ettikleri değerler (IC50) sırasıyla 17.11 μg/mL ve 124.7 μg/mL olarak tespit edilirken, α-amilaz enzimini sadece meyvenin inhibe etttiği (21.95 mg/mL) ve hem meyve hem de yaprakların lipaz inhibisyon aktivitesi göstermediği belirlenmiştir. Meyve ve yaprakların ekstraktlarının toplam fenolik, flavonoid ve tanen içerikleri ile antioksidan aktivite değerleri incelendiğinde meyvenin biyoaktif içeriğinin yaprağına göre anlamlı şekilde yüksek olduğu tespit edilmiştir (p<0.05). İğdenin meyve ve yapraklarının içerdiği biyoaktif bileşikler sayesinde göstermiş olduğu antidiyabetik aktivitenin in vitro çalışmalar sonrası elde edildiği göz önüne alındığında, iğdenin özellikle de meyve kısmının tip 2 diyabet rahatsızlıklarının doğal yoldan tedavisinde destekleyici yönünün olabileceği belirlenmiştir.

Anahtar Kelimeler

Elaeagnus angustifolia L., İğde, Biyoaktivite, Antidiyabet, Tanen

Antioxidant and Antidiabetic Properties of Oleaster (Elaeagnus angustifolia L.) Fruits and Leaves

Öz

This study was carried out to determine the antioxidant and antidiabetic activities of Elaeagnus angustifolia L. (oleaster) fruits and leaves. The total phenolic, total flavonoid and tannin contents, antioxidant activities (DPPH and ABTS methods), and inhibitory effects against α-glucosidase and α-amylase enzyme were investigated in the oleaster fruit and leaf extracts obtained with methanol:water (1:1, v/v). In addition, lipase inhibition activity was determined in these extracts. The values in which the fruit and leaves of oleaster inhibit the α-glucosidase enzyme by 50% (IC50) were determined as 17.11 μg/mL and 124.71 μg/L, respectively. Neither fruits nor leaves displayed a lipase inhibition activity. In terms of the phenolic, flavonoid and tannin contents and antioxidant activity values of the extracts of fruits and leaves, the bioactive content of fruits was significantly higher than that of leaves (p<0.05). Considering in vitro antidiabetic activities of the fruits and leaves of oleaster, the consumption of these natural extracts may have a potential for the treatment of type 2 diabetes.

Anahtar Kelimeler

Elaeagnus angustifolia L., Oleaster, Bioactivity, Antidiabetics, Tannin

Kaynakça

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