Yıl 2020, Cilt 18 , Sayı 1, Sayfalar 64 - 72 2020-04-30

Sünme (Rope) Problemi Olan Ekmeklerden İzole Edilen Bacillus Türlerinin Enterotoksin Üretme Potansiyeli
Enterotoxin Production Potential of Bacillus Species Isolated from Ropy Bread

Fundagül EREM [1] , Muharrem CERTEL [2] , Barçın KARAKAŞ BUDAK [3]


Bu çalışmada, daha önceki bir çalışmamızda sünme (rope) hastalığı oluşmuş ekmek içlerinden izole edilen Bacillus türleri, hemolitik (Hbl) ve hemolitik olmayan (Nhe) enterotoksin üretimleri açısından incelenmiştir. İncelenen toplam 39 izolattan sadece birinin hem Hbl hem de Nhe enterotoksinlerini, 22 izolatın sadece Hbl, 2 izolatın ise sadece Nhe enterotoksinini üretme kapasitesinin olduğu, kalan 14 izolatın ise her iki enterotoksini de üretmedikleri tespit edilmiştir. Enterotoksin analizleri için pozitif kontrol suşu olarak, her iki enterotoksini de ürettiği bilinen Bacillus cereus 2248 kullanılmıştır. Toksin analizleri, standart suşlar olan Bacillus subtilis PY22, B. subtilis RSK 244 ve B. subtilis RSK 246 için de yapılmış, aralarından sadece B. subtilis RSK 246’nın Nhe enterotoksinini üretme potansiyelinin olduğu belirlenmiştir. Ayrıca emetik toksin ürettiği bilinen Bacillus cereus 2455/2 suşu da Nhe enterotoksini açısından pozitif sonuç vermiştir. Sonuçlar, ekmeklerde sünme (rope) hastalığından sorumlu olan Bacillus türlerinin enterotoksin üretebileceğini gösterir niteliktedir.
In this study, Bacillus species isolated from ropy bread crumb in our previous study were investigated for the production of hemolytic (Hbl) and non-hemolytic (Nhe) enterotoxins. Of the 39 isolates, only one produced both Hbl and Nhe enterotoxins, 22 produced only Hbl, 2 produced only Nhe and the remaining 14 isolates were found to produce none of the two enterotoxins. Bacillus cereus 2248, which is known to produce both Hbl and Nhe, was used as the positive control strain for the enterotoxin assays. Toxin assays were also performed for the standard strains Bacillus subtilis PY22, B. subtilis RSK 244 and B. subtilis RSK 246. It was determined that amongst these strains only B. subtilis RSK 246 has the potential of producing the Nhe enterotoxin. Furthermore, the test results of Bacillus cereus 2455/2, which is known as an emetic strain, have shown that the strain was potentially positive in terms of Nhe enterotoxin. The results showed that Bacillus strains that are responsible for rope disease in bread could produce enterotoxins.
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Birincil Dil tr
Konular Gıda Bilimi ve Teknolojisi
Bölüm Araştırma Makaleleri
Yazarlar

Orcid: 0000-0003-1562-0686
Yazar: Fundagül EREM
Kurum: Bülent Ecevit Üniversitesi, Mühendislik Fakültesi, Gıda Mühendisliği Bölümü
Ülke: Turkey


Orcid: 0000-0002-1901-5590
Yazar: Muharrem CERTEL (Sorumlu Yazar)
Kurum: Akdeniz Üniversitesi, Mühendislik Fakültesi, Gıda Mühendisliği Bölümü
Ülke: Turkey


Orcid: 0000-0002-1426-3667
Yazar: Barçın KARAKAŞ BUDAK
Kurum: Akdeniz Üniversitesi, Mühendislik Fakültesi, Gıda Mühendisliği Bölümü
Ülke: Turkey


Destekleyen Kurum Akdeniz Üniversitesi Bilimsel Araştırmalar ve Koordinasyon Birimi
Proje Numarası 2010.03.0121.020
Teşekkür Bu çalışma, Akdeniz Üniversitesi Bilimsel Araştırmalar ve Koordinasyon Birimi tarafından 2010.03.0121.020 proje numarasıyla desteklenmiştir.
Tarihler

Yayımlanma Tarihi : 30 Nisan 2020

Bibtex @araştırma makalesi { akademik-gida730168, journal = {Akademik Gıda}, issn = {1304-7582}, eissn = {2148-015X}, address = {Fevzipaşa Bulv. Çelik İş Merkezi, No: 162, Kat: 3, D:302, Çankaya, İzmir}, publisher = {Sidas Medya Ajans Tanıtım Danışmanlık Ltd. Şti.}, year = {2020}, volume = {18}, pages = {64 - 72}, doi = {10.24323/akademik-gida.730168}, title = {Sünme (Rope) Problemi Olan Ekmeklerden İzole Edilen Bacillus Türlerinin Enterotoksin Üretme Potansiyeli}, key = {cite}, author = {EREM, Fundagül and CERTEL, Muharrem and KARAKAŞ BUDAK, Barçın} }
APA EREM, F , CERTEL, M , KARAKAŞ BUDAK, B . (2020). Sünme (Rope) Problemi Olan Ekmeklerden İzole Edilen Bacillus Türlerinin Enterotoksin Üretme Potansiyeli. Akademik Gıda , 18 (1) , 64-72 . DOI: 10.24323/akademik-gida.730168
MLA EREM, F , CERTEL, M , KARAKAŞ BUDAK, B . "Sünme (Rope) Problemi Olan Ekmeklerden İzole Edilen Bacillus Türlerinin Enterotoksin Üretme Potansiyeli". Akademik Gıda 18 (2020 ): 64-72 <https://dergipark.org.tr/tr/pub/akademik-gida/issue/54111/730168>
Chicago EREM, F , CERTEL, M , KARAKAŞ BUDAK, B . "Sünme (Rope) Problemi Olan Ekmeklerden İzole Edilen Bacillus Türlerinin Enterotoksin Üretme Potansiyeli". Akademik Gıda 18 (2020 ): 64-72
RIS TY - JOUR T1 - Sünme (Rope) Problemi Olan Ekmeklerden İzole Edilen Bacillus Türlerinin Enterotoksin Üretme Potansiyeli AU - Fundagül EREM , Muharrem CERTEL , Barçın KARAKAŞ BUDAK Y1 - 2020 PY - 2020 N1 - doi: 10.24323/akademik-gida.730168 DO - 10.24323/akademik-gida.730168 T2 - Akademik Gıda JF - Journal JO - JOR SP - 64 EP - 72 VL - 18 IS - 1 SN - 1304-7582-2148-015X M3 - doi: 10.24323/akademik-gida.730168 UR - https://doi.org/10.24323/akademik-gida.730168 Y2 - 2020 ER -
EndNote %0 Akademik Gıda Sünme (Rope) Problemi Olan Ekmeklerden İzole Edilen Bacillus Türlerinin Enterotoksin Üretme Potansiyeli %A Fundagül EREM , Muharrem CERTEL , Barçın KARAKAŞ BUDAK %T Sünme (Rope) Problemi Olan Ekmeklerden İzole Edilen Bacillus Türlerinin Enterotoksin Üretme Potansiyeli %D 2020 %J Akademik Gıda %P 1304-7582-2148-015X %V 18 %N 1 %R doi: 10.24323/akademik-gida.730168 %U 10.24323/akademik-gida.730168
ISNAD EREM, Fundagül , CERTEL, Muharrem , KARAKAŞ BUDAK, Barçın . "Sünme (Rope) Problemi Olan Ekmeklerden İzole Edilen Bacillus Türlerinin Enterotoksin Üretme Potansiyeli". Akademik Gıda 18 / 1 (Nisan 2020): 64-72 . https://doi.org/10.24323/akademik-gida.730168
AMA EREM F , CERTEL M , KARAKAŞ BUDAK B . Sünme (Rope) Problemi Olan Ekmeklerden İzole Edilen Bacillus Türlerinin Enterotoksin Üretme Potansiyeli. Akademik Gıda. 2020; 18(1): 64-72.
Vancouver EREM F , CERTEL M , KARAKAŞ BUDAK B . Sünme (Rope) Problemi Olan Ekmeklerden İzole Edilen Bacillus Türlerinin Enterotoksin Üretme Potansiyeli. Akademik Gıda. 2020; 18(1): 72-64.