Effect of Green Tea Extract and Whey Proteins on Experimental Gastric Ulcer Model in Rats

Yıl 2016, Cilt: 14 Sayı: 2, 111 - 122, 01.06.2016

Ayliz Velioğlu Öğünç Ahmet Özer Şehirli Berna Karakoyun Laçin Feriha Ercan Hülya Güçlü Güler Topçu

Öz

Green tea has multiple effects on human health while whey proteins exhibit antioxidantive and supporting effects on immunity. In this present study, preventive and curative effects of green tea and whey proteins were investigated on rat gastric ulcer model. To determine their effects, experiments were planned at two stages involving 10-dayadministration. Lipid peroxidation, glutathione, myeloperoxidase, reactive oxygen species, levels of TNF-α and IL-1β and histological evaluations were determined in collected tissues. As a result, combined consumption of these foods reduced the production of reactive oxygen species and lipid peroxidation, and this type of consumption had a synergistic effect for the prevention of gastric ulcer in a rat model. Biochemical and histological data in assessed tissue damages were also compatible. In conclusion, although their curative and preventive properties for gastric ulcer were significant, combined consumption of green tea and whey proteins can be considered as “functional food alternative” to support clinical therapy; however, they should not replace drug therapies

Anahtar Kelimeler

Gastric ulcer, Whey proteins, Green tea, Oxidative damage, Functional foods

Sıçanlarda Deneysel Mide Ülseri Modelinde Yeşil Çay Ekstraktı ve Peynir Altı Suyu Proteinlerinin Etkileri

Öz

Bu çalışmada, çok yönlü tedavi edici etkileri tartışılan yeşil çay ile antioksidan ve bağışıklık sistemini destekleyen etkileriyle ön plan çıkan peynir altı suyu proteinlerinin, sıçanlarda asetik asit uygulamasıyla oluşturulan mide ülseri modeli üzerinde koruyucu ve iyileştirici etkilerinin aydınlatılması amaçlanmıştır. Her iki gıdanın ülser üzerine etkilerini araştırmak amacıyla, on günlük uygulamaları içeren iki hayvan deneyi aşaması oluşturulmuştur. Toplanan dokularda, lipid peroksidasyon, glutatyon, miyeloperoksidaz enzim aktivitesi, reaktif oksijen türleri, Tümör Nekroz Faktör-α ve İnterlökin-1β ölçümleri ile histolojik değerlendirmeler yapılmıştır. Çalışma sonucunda, peynir altı suyu proteinlerinin ve yeşil çayın birlikte tüketimiyle, ülser nedeniyle oluşacak reaktif oksijen türleri üretimini ve lipid peroksidasyonunu tek başlarına kullanımlarına göre daha fazla önlediği saptanmıştır. Doku hasarını belirleyen bulgular ve histolojik değerlendirmeler de bu sonucu desteklemiştir. Sonuç olarak, mide ülseri için iyileştirici ve önleyici özellikleri belirgin olsa da, yeşil çay ve peynir altı suyu proteinlerinin klinik uygulamaları destekleyen ”fonksiyonel gıda alternatifi” olabileceği, ancak ilaç tedavilerinin önüne geçemeyeceği görülebilir

Anahtar Kelimeler

Mide ülseri, Peynir altı suyu proteinleri, Yeşil çay, Oksidatif hasar, Fonksiyonel gıdalar

Kaynakça

• Başer, K.H.C., 2003. Industrial plants as sources of dietary supplements, in: Dietary supplements of plants origin, ed. M. Maffei, Taylor and Francis, London, pp 31-42.
• De la Fuente, M.A., Hemar, Y., Tamehana, M., Munro, P.A., Singh, H., 2002. Process induced changes in whey proteins during the manufacture of whey protein concentrates. Int. Dairy. J. 12: 361- 369.
• Sang, S., Tian, S., Wang, H., Stark, R.E., Rosen, R.T., Yang, C.S., Ho, C.T., 2003. Chemical studies of the antioxidant mechanism of tea catechins. Bioorganic& Medicinal Chemistry 11: 3371-3378.
• Bounous, G, 2000. Whey protein concentrate (WPC) and glutathione modulation in cancer treatment. Anticancer Res. 20: 4785-4792. [5] Grossman, M.I., 1980. background, Scand I pathophysiological Gastroenterology 15(Suppl 58):7-15.
• Pinhan, G., Regilo, G., Szabo, S., 1987. Free radicals and lipid peroxidation in ethanol or aspirin induced gastric mucosal injury. Dig. Dis. Sci. 32: 1395-1401.
• Biswas, K., Bandyopadhyay, U., Chattopadhyay, I., Varadaraj, A., Ali, E., Banerjee, RK., 2003. A novel antioxidant and antiapoptotic role of omeprazole to block gastric ulcer through scavenging of hydroxyl radical. J. Biol. Chem. 278: 10933-11001.
• Jahoviç, N., Velioğlu Öğünç, A., Güzel, E., Ars, D., Ercan, F., Erkanlı,, G., Yeğen, B.Ç, Yalçın, A.S., 2005. Whey pretreatment ameliorates gastric and hepatic oxidative damage in ethanol-induced gastric ulcer via a neutrophil-dependent mechanism. Marmara Medical Journal 18: 64-70.
• Okabe, S., Pfeiffer, C.J., 1972. Chronicity of acetic acid ulcer in the rat stomach. Am. J. Dig Sis. 7: 619- 629.
• Konturek, S.J, Brzozowski, T., Dembinaki, A., Warzecha,, Z., Konturek, P.K., Yanaihara, N.,1988. Interaction of growth hormone-releasing factor and somatostatin on ulcer healing and mucosal growth in rats. Role of gastrin epidermal growth factor. Digestion 41: 121-128.
• Okabe, S., Pfeiffer, C.J., Roth, I.L.A., 1971. A method for experimental penetrating ulcer in rats. Am. J. Dig. Dis. 916: 277-284.
• Öner, O.Z., Öğünç, A.V., Cingi, A., Uyar, S.B., Yalçın, A.S., Aktan, A.O., 2006. Whey feeding suppresses the measurements of oxidative stress experimental burn injury. Surgery Today, 36:376-81.
• Bradley, P.P., Priebat, D.A., Christerser, R.D., Rothstein, G., 1982. Measurement of cutaneous inflammation: Estimation of neutrophil content with an enzyme marker. J. Invest. Dermatol. 78: 206- 209.
• Casini, A., Ferrali, M., Pompella, A.S., Maellaro, E., Comporti, M., 1986. Lipid peroxidation and cellular damage in extrahepatic tissues of bromobenzene toxicated mice. Am. J. Pathol. 123: 520-531.
• Aykaç, G., Uysal, M., Yalçın, A.S., Koçak-Toker, N., Sivas, A., Öz, H., 1985. The effect of chronic ethanol ingestion on hepatic lipid peroxide, glutathione peroxidase and glutathione transferase in rats. Toxicology 36: 71-76.
• Haklar, G., Özver, E.S., Yüksel, M., Aktan, A.Ö., Yalçın, A.S., 2001. Different kinds of reactive oxygen and nitrogen species were detected in colon and breast tumors. Cancer Letters 165: 219-224.
• Russel, R.J., 1990. Endoscopic evaluation of etodolac and naproxen and their relative effects on gastric and duodenal prostaglandins. Rheumatol Int. 10: 17-21.
• Ensminger, A.H., Ensminger, M.E., Konlande, J.E., Robson, J.R.K., 1995. Milk and milk products. The Concise encylopedia of foods and nutrition. CRC Press Inc, U.S.A, p: 691-710.
• Lindmark-Mansson, H., Akesson, B., 2000. Antioxidative factors in milk. Br. J. Nutr. (Suppl-1), 84: 103-110.
• Butriss, J., Milk. Ed: Macrae, R., Robinson, R.K., Sadler, M.J., 1993. Encyclopaedia of food science, food technology and nutrition, Academic Press, London, San Diego., Vol: 5, pp.:3066-3090.
• Bounous, G., Kongshavn, P.A.T., 1978. The effect of dietary amino acids on immune reactivity. Immunology 35: 257-266.
• Bounous, G., Papenburg, R., Kongshavn, P.A.T., Gold, Fleiser, D., 1988. Dietary whey proteins inhibits the development of dimethylhydrazine induced malignancy. Clin. Invest. Med. 11: 213-217.
• Lothian, B., Grey, V., Kimoff, R.J., Lands, L.C., 2000. Treatment of obstructive airway disease with a cysteine donor protein supplement. Chest 117: 914-916.
• Melgarejo, E., Medina, M.A., Sanchez Jimenez, F., Urdiales, J.L, 2010. Targeting of histamine producing cells by EGCG: a green dart against inflammation J. Physiol Biochem. 66: 265-270.
• Hou, C., Amarnani, S., Chong, M.T., Bishayee, A., 2013. Green tea and the risk of gastric cancer: Epidemiological evidence, World J. Gastroenterol. 19(24): 3713-3722. [26] Darvesh, A.S., Bishayee, A, 2013. Chemopreventive and therapeutic potential of tea polyphenols in hepatocellular cancer. Nutrition and Cancer 65(3): 329-344.
• Varliek, G.W., Yang, F., Lee, E.Y., de Villiers, W.J., Zhory, J., Oz, H.S., Westberry, K.F., Mc Clain, C.J., 2001. Green Tea polyphenol extract attenuates inflammation in interleukin-2 deficient mice, a model of autoimmunity. J. Nutr. 131(7): 2034-9.
• Lee, S.Y., Shin, Y.W., Hahm, K.B., 2008. Phytoceuticals: Mighty but ignored weapons against Helicobacter pylori infection. J. Digest. Dis. 9: 129- 139.
• Adhikary, B., Yadav, S.K., Bandyopadhyay, S.K., Chattopadhyay, S., 2011. Epigallocatechin gallate accelerates healing stomach ulcers in mice. Pharmacogicals Reports 63: 527-536.
• indomethacin-induced
• Vordenbaumen, S., Braukmann, A., Petermann, K., Schaf, A., Bleck, E., Von Mikelz, A., Jose, J., Schneider, M., 2011. Casein αs1 is expressed by human monocytes and upregulates the production of GM-CSF via p 38. J. Immunol. 186(1): 592-601.
• Rusu, D., Droin, R., Pouliot, Y., Gauthier, S., Poubelle, P.E., 2010. A bovine whey protein extract stimulates human neutrophils to generate bioactive IL-Ra through a NF- kappa B and MAPK dependent mechanism. J. Nutr. 140(2): 382-91.
• Dube, P.E., Brubaker, P.L., 2007. Frontiers in Glucagon-like peptide 2: Multiple actions, multiple mediators. Am. J. Physiol. Endocrinol. Metab. 293(2): 460-465.
• Chatterton, D.E.W., Nguyen, D.N., Bering, S.B., Sangild, P.T, 2013. Antiinflammatory mechanisms of bioactive milk proteins in the intestine of newborns. Int. J. Biochem. Cell. Bio. 45: 1730-1747.
• Monnai, M., Horimoto, Y., Otani, H., 1998. Immunomodificatory effect of dietary bovine kappa- caseinoglycopeptide on serum antibody levels and proliferative responses of lymphocytes in mice. Milchwissenschaft 53: 129-132.
• Lopez-Posadas, R., Requena, P., Gonzales, R., Suarez, M.D., Zarzuelo, A., Sanchez, M., 2010. Bovine antiinflammatory effects in rats with dextran sulfate- induced colitis. J. Nutr. 140: 2014-2019. has intestinal
• Rainsford, D., 1978. The role of aspirin in gastric ulceration. development of gastric mucosal damage induced by aspirin in rats exposed to various stress conditions. Am. J. Dig. Dis. 23(6): 521-530. involved in the
• Ebaid, H., Ahmed, O.M., Mahmoud, A.M., Ahmed, R.R., 2013. Limiting prolonged inflammation during proliferation and remodeling phases of wound healing in streptozotocin- induced diabetic rats supplemented with camel undenatured whey protein. BMC Immunology 14: 31.
• Kerasioti, E., Stagos, D., Jamurtas, A., Kiskini, A., Koutedakis, Y., Goutzourelas, N., Pournaras, S., Tsatsakis, A.M., Kouretas, D., 2013. Anti- inflammatory effects of a special carbohydrate-whey protein cake after exhaustive cycling in human. Food and Chemical Toxicology (61): 42-46.
• Uchiyama, Y., Suzuki, T., Mochizuki, K., Goda, T., 2013. epigallocatechin-3-gallate reduces inflammatory response in adipose tissue of non-obese type 2 diabetic Goto-Kakizaki(GK) rats. J. Agric. Food Chem. 61: 11410-1417. with (-)
• Mazzon, E., Muia, C., Di Paola, R., Genovese, T., Menegazzi, M., De Sarro, A., Suzuki, H., Cuzzocrea, S., 2005. Green tea polyphenol extract attenuates colon injury induced by experimental colitis. Free Radical Research 39(9): 1017-1025.
• Khan, A.S., Priyamda, S., Arivarasu, N., Khan, S., Yusufi, A.N.Y., 2007. Influence of green tea on enzymes of carbohydrate metabolism, antioxidant defense, and plasma membrane in rat tissues. Nutrition 23: 687-695.
• Ostrowska, J., Luczaj, W., Kasacka, I., Rozanski, A., Skrzydlewska, E., 2004. Green tea protects against ethanol-induced lipid peroxidation in rat organs. Alcohol 32: 25-32.
• Bounous, G., Batist, G., Gold, P., 1989. Immunoenhancing property of dietary whey protein in mice: role of glutathione. Clin. Invest. Med. 12: 154-161.
• Beutler, E., 1989. Nutritional and metabolic aspects of glutathione. Ann. Rev. Nutr. 9: 287-302.