Gıda ve Yemlerden İzole Edilen Farklı Enterococcus Türlerinin Antibiyotik Dirençlilikleri

Yıl 2013, Cilt: 11 Sayı: 3-4, 52 - 57, 01.09.2013

Bülent Baş Ahmet Koluman Teoman Ünlü

Öz

Bu çalışmada, Ankara ve Bolu illerinde bulunan broiler piliç çiftliklerinden alınan yem ve piliç kesimhanelerinden alınan et örnekleri ile süt üretimi yapan sığır çiftliklerinden alınan yem ve toplama tankı süt örnekleriyle çalışıldı. İzole edilen 31 Enterococcus faecium ve 34 Enterococcus faecalis suşlarının ampisilin, penisilin, eritromisin, tetrasiklin, kloramfenikol, rifampisin, siprofloksasin, levofloksasin, vankomisin, teikoplanin, nitrofrantoin, gentamisin, streptomisin ve kuinupristin/dalfopristin diskleri kullanılarak antibiyotik dirençliliklerine bakıldı. Vankomisin dirençliliği, 31 E. faecium suşundan 4’ünde %12.9 ve 34 E. faecalis suşundan 3’ünde %8.8 bulundu

Anahtar Kelimeler

Enterokok, Antibiyotik dirençliliği, Yem, Süt

Antibiotic Resistance of Enterococcus spp. Isolated from Food and Feed Samples

Öz

In this study different feed materials from broiler chicken establishments, meat samples from chicken slaughterhouses and milk samples of tank bulk from dairy farmsin Ankara and Bolu Turkey regions were evaluated. Isolated Enterococcus faecium 31 and Enterococcus faecalis 34 strains were examined for their antibiotic resistance against ampicillin, penicillin, erythromycin, tetracycline, chloramphenicol, rifampicin, ciprofloxacin, levofloxacin, vancomycin, teicoplanin, nitrofurantoin, gentamicin, streptomycin and quinupristin/dalfopristin antibiotics. Vancomycin resistance were found in 4 12.9 % and 3 8.8 % out of 31 E. faecium and 34 E. faecalis strains, respectively

Anahtar Kelimeler

Enterococci, Antibiotic resistance, Feed, Milk

Kaynakça

• Anonymous, 2012. Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing; 16th Informational Supplement. CLSI Document M100– S22. Clinical and Laboratory Standards Institute, Wayne, PA.
• Channaiah, L.H., Subramanyam, B., Zurek, L., 2010. OG1RF:pCF10 in poultry and cattle feed: vector competence of the red flour beetle, Tribolium castaneum (Herbst). J. Food Prot. 73(3): 568–573.
• Çiftçi, A., Fındık, A., İça, T., Baş, B., Onuk, E.E., Güngördü, S., 2009. Slime production and antibiotic resistance of Enterococcus faecalis isolated from arthritis in chickens. J. Vet. Med. Sci., 71 (6): 849- 853.
• Dağdemir, E., Özdemir, S. 2006. Süt ve mamüllerinde Enterokoklar. Türkiye 9. Gıda Kongresi; 24-26 Mayıs 2006, Bolu.
• Devriese, L.A., Hommez, J., Laevens, H., Pot, B., Vandamme, Identification of aesculin-hydrolyzing Streptocci, Lactococci, Aerococci and Enterococci from subclinical intramammary infections in dairy cows. Vet. Microbiol. 70: 87-94. F., 1999.
• Fracalanzza, S.A.P., Scheidegger, E.M.D., Santos, P.F., Leite, P.C., Teixeira, L.M., 2007. Antimicrobial resistance profiles of enterococci isolated from poultry meat and pasteurized milk in Rio de Janeiro, Brazil. Mem. Inst. Oswaldo Cruz. 102(7): 853-859.
• Graham, J.P., Price, L.B., Evans, S.L., Graczyk, T.K., Silbergeld, E.K. 2009. Antibiotic resistant enterococci and staphylococci isolated from flies collected near confined poultry feeding operations. Sci.Total Environ. 407(8): 2701-2710.
• Harwood, V.J., Brownell, M., Perusek, W., Whitlock, J.E., 2001. Vancomycin-resistant Enterococcus spp. isolated from wastewater and chicken feces in the United States. Appl. Environ. Microbiol. 67: 4930-4933.
• Hassan, L., Getachew, Y.M., Zunati, Z., Kamaruddin, M.I., 2008. Distribution of Van Genes of Vancomycin-resistant Enterococcus Isolated from Broilers in Peninsular Malaysia. Proceedings, The 15th Congress of FAVA. O11-O12.
• Hayes, J.R., English, L.L., Carter, P.J., Proescholdt, T., Lee, K.Y., Wagner, D.D., White, D.G. 2003. Prevalence and antimicrobial resistance of Enterococcus species isolated from retail meats. Appl. Environ. Microbiol. 69(12): 7153-7160.
• Jung, W.K., Lim, J.Y., Kwon, N.H., Kim, J.M., Hong, S.K., Koo, H.C., Kim, S.H., Park, Y.H. 2007. Vancomycin-resistant enterococci from animal sources in Korea. Int. J. Food Microbiol. 113: 102- 107.
• Kaleli, D., Durlu-Özkaya, F., 2000. Enterekok Aranması pp.387-394. In: Gıda Mikroobiyolojisi ve Uygulamaları. 2. Baskı, Sim Matbaacılık, Ankara.
• Lemcke, R., Bülte. M., 2000. Occurrence of the vancomycin-resistant genes vanA, vanB, vanC1, vanC2 and vanC3 in Enterococcus strains isolated from poultry and pork. Int. J. Food Microbiol. 60: 185-194.
• Martins da Costa, P., Oliveira, M., Bica, A., Vaz- Pirez, P., Bernardo, F., 2007. Antimicrobial resistance in Enterococcus spp. and Escherichia coli isolated from poultry feed and feed ingredients. Vet. Microbiol. 120: 122-131.
• McDermott, P.F., Cullen, P., Hubert, S.K., McDermott, S.D., Bartholomew, M., Simje, S., Wagner, D.D., 2005. Changes in antimicrobial susceptibility of native Enterococcus faecium in chickens fed virginiamycin. Appl. Environ. Microbiol. 71(9): 4986-4991.
• Moreno, M.R., Sarantinopoulos, P., Tsakalidou, E., De Vuyst, L., 2006. The role and application of enterococci in food and health. Int. J. Food Microbiol. 106: 1-24.
• Murcia, A.J., Collins, M.D. 1991. Enterococcus sulfureus, a new yellow-pigmented Enterococcus species. FEMS Microbiol. Lett. 64: 69-74.
• Nam, H.K., Lim, S.K., Moon, J.S., Kang, H.M., Kim, J.M., Jang, K.C., Kim, J.M., Kang, M.I., Joo, Y.S., Jung, S.C., 2010. Antimicrobial resistance of Enterococci isolated from mastitic bovine milk samples in Korea. Zoonozes Public Health 57: e59- e64.
• Pattel, R., Uhi, J.R., Kohner, P., Hopkins, M.K., Cockerill, F.R., 1997. Multiplex PCR detection of Van A, Van B, Van C-1 and Van C-2/3 genes in enterococci. J. Clin. Microbiol. 35(3): 703-707.
• Pettersson-Wolfe, C.S., Adams, S., Wolf, S.L., Hogan, J.S. 2008. Genomic typing of Enterococci isolated from bovine mammary glands and environmental sources. J. Dairy Sci. 91(2): 615- 619.
• Riberio, T., Abrantes, M., Lopes, M.F.S., Crespo, M.T.B., 2007. Vancomycin-susceptible dairy and clinical enterococcal isolates carry vanA and vanB genes. Int. J. Food Microbiol. 113: 289-295.
• Schleifer, K.H., Balz, R., 1984. Transfer of Streptococcus faecalis and Streptococcus faecium to the genus Enterococcus nom. rev. as Enterococcus Enterococcus faecium comb. nov. Int. J. Syst. Bacteriol. 34: 31-34. comb. nov. and
• Sırıken, B., Fındık, A., İnat, G., Çadırcı, Ö., Kevenk, T.O., 2011. The investigation of prevalence, vancomycine resistance and slime factor production of enterococci isolated from chicken carcasses. Etlik Vet. Mikrobiyol. Derg. 22: 54-60.
• Simje, S., White, D.G., Meng, G., Wagner, D.D., Qaiyumi, S., Zhao, S., Hayes, J.R., McDermott, P.F., 2002. Prevalence of streptogramin resistance genes among Enterococcus isolates recovered from retail meats in the Greater Washington DC area. J. Antimicrob. Chemother. 50: 877-882.
• Tenhagen, B.A., Köster, G., Wallmann, J., Heuwieser, W., 2006. Prevalence of mastitis pathogens antimicrobial agents in dairy cows in Brandenburg, Germany. J. Dairy Sci. 89: 2542-2551. against
• Turtura, G.C., Lorenzelli, P., 1994. Gram-positive cocci isolated from slaughtered poultry. Microbiol. Res. 149: 203-213.