Yıl 2020, Cilt 18 , Sayı 3, Sayfalar 303 - 311 2020-10-29

Utilization of CRISPR/Cas System of Lactic Acid Bacteria in Biotechnology and Genetic Engineering
Laktik Asit Bakterilerinde CRISPR/Cas Sisteminin Biyoteknoloji ve Genetik Mühendisliğinde Kullanımı

Özge KAHRAMAN ILIKKAN [1]


Lactic acid bacteria (LAB) are a group of low-GC content, Gram-positive, facultative anaerob, non-motile, non-spore-forming, acid tolerant bacteria that can ferment various nutrients. This group of bacteria mostly contains starter cultures and probiotics. Discovery of “Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)” and “CRISPR-related Cas” proteins accelerated studies pertaining to the subject and provided a simplified genom editing mechanism. Genom editing via CRISPR/Cas system and combination of this system with other genetic tools will break new ground on industrial and clinical applications of lactic acid bacteria and probiotics. This review aims to provide an insight on how CRISPR/Cas system works, which types of CRISPR/Cas system lactic acid bacteria and probiotics contain, how it is applied to biotechnology and genetic engineering as well as the future potential applications.
Laktik asit bakterileri (LAB) düşük GC içeriğine sahip, Gram pozitif, spor oluşturmayan, hareketsiz, fakültatif anaerob, asidik ortama dayanıklı ve çeşitli besinleri fermente etme yeteneğindeki bakterilerden oluşan bir gruptur. Bu grup genellikle probiyotik ve starter kültür olarak kullanılan bakterileri içerir. “Düzenli aralıklarla kümelenmiş kısa palindromik tekrarlar (CRISPR)” ve “CRISPR ilişkili Cas proteinleri”den oluşan CRISPR/Cas sisteminin keşfi ile bu konuda yapılan çalışmalar hız kazanmış ve genom düzenlemeleri kolayca yapılmaya başlanmıştır. Söz konusu sistem yardımıyla yapılan genom düzenlemeleri ve sistemin diğer genetik mühendisliği yöntemleriyle birleştirilmesi, LAB’ın ve probiyotiklerin endüstri ve klinikte kullanımına yönelik yeni bir çığır açacaktır. Bu derleme, CRISPR/Cas sisteminin genel işleyişi, LAB’ta hangi sistemlerden oluştuğu, biyoteknoloji ve genetik mühendisliğindeki mevcut uygulamaları ile gelecekteki potansiyel uygulamaları konusunda geniş bir bakış açısı sağlayacaktır.
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Birincil Dil tr
Konular Gıda Bilimi ve Teknolojisi
Bölüm Derleme Makaleler
Yazarlar

Orcid: 0000-0001-5843-6868
Yazar: Özge KAHRAMAN ILIKKAN (Sorumlu Yazar)
Kurum: Başkent Üniversitesi, Kahramankazan Meslek Yüksek Okulu, Gıda İşleme Bölümü
Ülke: Turkey


Tarihler

Yayımlanma Tarihi : 29 Ekim 2020

Bibtex @derleme { akademik-gida818183, journal = {Akademik Gıda}, issn = {1304-7582}, eissn = {2148-015X}, address = {Fevzipaşa Bulv. Çelik İş Merkezi, No: 162, Kat: 3, D:302, Çankaya, İzmir}, publisher = {Sidas Medya Ajans Tanıtım Danışmanlık Ltd. Şti.}, year = {2020}, volume = {18}, pages = {303 - 311}, doi = {10.24323/akademik-gida.818183}, title = {Laktik Asit Bakterilerinde CRISPR/Cas Sisteminin Biyoteknoloji ve Genetik Mühendisliğinde Kullanımı}, key = {cite}, author = {Kahraman Ilıkkan, Özge} }
APA Kahraman Ilıkkan, Ö . (2020). Laktik Asit Bakterilerinde CRISPR/Cas Sisteminin Biyoteknoloji ve Genetik Mühendisliğinde Kullanımı . Akademik Gıda , 18 (3) , 303-311 . DOI: 10.24323/akademik-gida.818183
MLA Kahraman Ilıkkan, Ö . "Laktik Asit Bakterilerinde CRISPR/Cas Sisteminin Biyoteknoloji ve Genetik Mühendisliğinde Kullanımı" . Akademik Gıda 18 (2020 ): 303-311 <https://dergipark.org.tr/tr/pub/akademik-gida/issue/57602/818183>
Chicago Kahraman Ilıkkan, Ö . "Laktik Asit Bakterilerinde CRISPR/Cas Sisteminin Biyoteknoloji ve Genetik Mühendisliğinde Kullanımı". Akademik Gıda 18 (2020 ): 303-311
RIS TY - JOUR T1 - Laktik Asit Bakterilerinde CRISPR/Cas Sisteminin Biyoteknoloji ve Genetik Mühendisliğinde Kullanımı AU - Özge Kahraman Ilıkkan Y1 - 2020 PY - 2020 N1 - doi: 10.24323/akademik-gida.818183 DO - 10.24323/akademik-gida.818183 T2 - Akademik Gıda JF - Journal JO - JOR SP - 303 EP - 311 VL - 18 IS - 3 SN - 1304-7582-2148-015X M3 - doi: 10.24323/akademik-gida.818183 UR - https://doi.org/10.24323/akademik-gida.818183 Y2 - 2020 ER -
EndNote %0 Akademik Gıda Laktik Asit Bakterilerinde CRISPR/Cas Sisteminin Biyoteknoloji ve Genetik Mühendisliğinde Kullanımı %A Özge Kahraman Ilıkkan %T Laktik Asit Bakterilerinde CRISPR/Cas Sisteminin Biyoteknoloji ve Genetik Mühendisliğinde Kullanımı %D 2020 %J Akademik Gıda %P 1304-7582-2148-015X %V 18 %N 3 %R doi: 10.24323/akademik-gida.818183 %U 10.24323/akademik-gida.818183
ISNAD Kahraman Ilıkkan, Özge . "Laktik Asit Bakterilerinde CRISPR/Cas Sisteminin Biyoteknoloji ve Genetik Mühendisliğinde Kullanımı". Akademik Gıda 18 / 3 (Ekim 2020): 303-311 . https://doi.org/10.24323/akademik-gida.818183
AMA Kahraman Ilıkkan Ö . Laktik Asit Bakterilerinde CRISPR/Cas Sisteminin Biyoteknoloji ve Genetik Mühendisliğinde Kullanımı. Akademik Gıda. 2020; 18(3): 303-311.
Vancouver Kahraman Ilıkkan Ö . Laktik Asit Bakterilerinde CRISPR/Cas Sisteminin Biyoteknoloji ve Genetik Mühendisliğinde Kullanımı. Akademik Gıda. 2020; 18(3): 303-311.
IEEE Ö. Kahraman Ilıkkan , "Laktik Asit Bakterilerinde CRISPR/Cas Sisteminin Biyoteknoloji ve Genetik Mühendisliğinde Kullanımı", Akademik Gıda, c. 18, sayı. 3, ss. 303-311, Eki. 2020, doi:10.24323/akademik-gida.818183