Multi-Drug Resistance-1 [MDR1, P-gp, ABCB1] Gene Polymorphism in Patients with Abdominal Aortic Aneurysm and Their Family Members

Abstract

Objective: The aim of this study was to investigate the relationship between the 3435C>T polymorphism in the multi-drug resistance-1 gene [MDR1, P-gp, ABCB1] and the development of abdominal aortic aneurysm AAA .Material and Methods: This study included 75 patients with AAA Group I , 75 individuals who were first-degree relatives of these patients but did not have AAA diagnosis Group II , and 75 healthy volunteers whose abdominal aortic diameters were in the normal range Group III . Genomic DNAs of these individuals were obtained from the peripheral blood. The MDR1:c.3435C>T polymorphism [CC Wild Type , CT Heterozygous and TT Homozygous genotypes] was investigated by using the real-time polymerase chain reaction technique real-time PCR .Results: MDR1 TT genotype was significantly more frequent in Groups I and II compared to Group III 33.3% and 38.6% vs. 8%, respectively, p

Keywords

• Abdominal aortic aneurysm, MDR1 gene polymorphism

Abdominal Aort Anevrizması Bulunan Hastalarda ve Aile Üyelerinde Multi-Drug Resistance-1 [MDR1, P-gp, ABCB1] Geni Polimorfizmi

Öz

Amaç: Bu çalışmanın amacı Multi-drug resistance-1 [MDR1, P-gp, ABCB1] genindeki 3435C>T polimorfizmi ile abdominal aort anevrizması AAA gelişimi arasındaki ilişkinin incelenmesidir.Gereç ve Yöntemler: AAA tanısı olan 75 hasta Grup I , bu hastaların AAA tanısı olmayan birinci derece akrabalarından oluşan 75 gönüllü Grup II ve aort çapları normal olan ve akrabaları olmayan 75 sağlıklı gönüllü Grup III çalışmaya alındı. Periferik kandan olgulara ait genomik DNA’lar elde edildi. MDR1:c.3435C>T polimorfizmi [CC Doğal genotip , CT Heterozigot ve TT Homozigot genotipler] eş zamanlı polimeraz zincir reaksiyonu real-time PCR tekniği kullanılarak araştırıldı.Bulgular: MDR1 TT genotipinin Grup I ve Grup II’de kontrol grubuna göre anlamlı derecede fazla olduğu görüldü sırasıyla %33,3 ve %38,6’e karşı %8, p

Anahtar Kelimeler

• Abdominal aort anevrizması, MDR1 gen polimorfizmi

Kaynakça

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