Gas Chromatography-Mass Spectrometry Determination of Organochlorine Pesticides in Various Plant Leaves Used as Biomonitors in Environmental Pollution, Ergene River Basin, Turkey

Yıl 2018, Cilt: 18 Sayı: 1, 222 - 232, 30.04.2018

Cemile Özcan

Öz

Ergene Basin is one of the 23 basins which identified the borders in Turkey. This Basin covers a large part of the province of Tekirdağ, Edirne and Kırklareli.The various plant samples (Urtica dioica L., Melissa officinalis, Verbascum sinuatum, Typha latifolia L., Pinus nigra L., Cupressus arizonica L., Populus nigra L., Cedrus libani L., Paliurus aculeatus L., Mentha pulegium L., Plantago major L., Laurus nobilis L.) collected from ten point in Ergene River Basin during 2012 year were extracted by using the quick, easy, cheap, effective, rugged and safe (QuEChERS) extraction method. The concentrations of organochlorine pesticides having high toxicity were determined by using gas chromatography-mass spectrometry with electron impact ionization-possitive mode (GC-MS-EI (+)). The LOD, LOQ and RSD% were between 0.02- 0.21 μg L-1 , 0.06-0.71 μg L-1 , below 10%. for pesticides, respectively. Recoveries of pesticides in plant samples ranged from 78.9% to 99.9%, respectively. The ΣHCH and Dieldrin concentrations were not exceeded the tolerable daily intake limit accordingly serving as likely threat to humans

Anahtar Kelimeler

Ergene River Basin, plant samples, pesticides, GC-MS, QuEChERS

Türkiye, Ergene Nehir Havzası'nda, Çevre Kirliliğinde Biomonitör Olarak Kullanılan Çeşitli Bitki Yapraklarındaki Organoklorlu Pestisitlerin Gaz Kromatografisi-Kütle Spektroskopisi ile Tayini

Öz

Ergene Havzası Türkiye'de sınırları belirlenmiş olan 23 havzadan birisidir. Ergene Havzası, Tekirdağ, Edirne ve Kırklareli illerinin büyük bir kısmını kapsamaktadır. 2012 yılı süresince Ergene Nehir Havzasında on noktadan toplanan çeşitli bitki örnekleri (Urtica dioica L., Melissa officinalis, Verbascum sinuatum, Typha latifolia L., Pinus nigra L., Cupressus arizonica L., Populus nigra L., Cedrus libani L., Paliurus aculeatus L., Mentha pulegium L., Plantago major L., Laurus nobilis L.) hızlı, kolay, ucuz, etkili, dayanıklı ve güvenli (QuEChERS) ekstraksiyon metodu kullanılarak ekstrakte edilmiştir. Yüksek toksisiteye sahip organik klorlu pestisitlerin konsantrasyonları gaz kromatografi-kütle spektroskopisi-elektron impact iyonlaştırma-pozitif mod (GC-MS-EI (+)) kullanılarak tayin edilmiştir. Pestisitler için sırasıyla LOD, LOQ ve RSD% değerleri 0.02-0.21 μg L-1, 0.06-0.71 μg L-1 ve altında %10 bulunmuştur. Bitki örneklerindeki pestisitlerin geri dönüşüm çalışmaları sırasıyla 78.9-99.9% aralığında değişmektedir. İnsan sağlığını tehlikeye sokan ΣHCH ve Dieldrin konsantrasyonlarında izin verilen günlük alım sınırını aşmadığı belirlenmiştir.

Anahtar Kelimeler

Ergene Nehir Havzası, bitki örnekleri, pestisitler, GC-MS; QuEChERS.

Kaynakça

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