YUMURTACI TAVUK YEMLERİNE İLAVE EDİLEN KEKİK OTUNUN (Thymus Vulgaris) YUMURTA SARISI VE KAN SERUMUNDA TRİGLİSERİD VE KOLESTEROL ORANI İLE DIŞKIDA E.COLİ YOĞUNLUĞU ÜZERİNE ETKİSİ

Öz

Bu çalışma, yumurta tavuk diyetlerine ilave edilen kekik (Tymus vulgaris) otunun kan serumu ve yumurta sarısında trigliserid ve kolesterol seviyesi ile dışkıda E.coli sayısı üzerine etkisini araştırmak amacıyla yapılmıştır. Çalışmada 24 haftalık yaşta 64 adet Lohmann LSL hibrit yumurtacı tavuk dört gruba ayrılmıştır. Her grup dört tekerrürlü olarak yürütülmüştür. Diyetler, bazal yeme sırasıyla % 0, 0,1, 0,5 ve 1 oranında kekik otu ilave edilerek hazırlanmıştır. Yemlere katılan kekik otu yumurta sarısı trigliserid ve kolesterol oranını etkilemezken, % 0.1 kekik otu ilavesinin kan serumu trigliserid ve kolesterol seviyesini önemli derecede düşürdüğü tespit edilmiştir. Yumurta tavuğu diyetlerinde % 0,1 ve 0,5 düzeyinde kekik otu kullanılması dışkıda E.coli sayısını önemli derecede azaltmıştır.

Anahtar Kelimeler

• Yumurtacı tavuk, Kekik, Trigliserid, Kolesterol, E.coli

Kaynakça

1. Alçiçek, A., Bozkurt, M., Çabuk, M.2003. The effect of an essential oil combination derived from selected herbs growing wild in Turkey on broiler performance. South Afr. J. Anim. Sci., 33 (2): 89-94.
2. Anonymous, 1992. A possible way to count coliform bacteria groups and E. coli numbers in feedstuffs and diets. A possible number (MPN). Turkish Official Newspaper, Jan 21, No: 21118.
3. Botsoglou, N.A., Fletouris, D.J. 2001. Drug Residues in Foods. Pharmacology, Food Safety and Analysis. New York, Marcel Dekker, Inc. pp.541-548.
4. Botsoglou, N.A., Florou-Paner, P., Christaki, E., Fletouris, D.J., Spais, A.B. 2002. Effect of dietary oregano essential oil on performance of chickens and on iron- induced lipid oxidation of breast, thigh and abdominal fat tissues. Bri. Poult. Sci., 43:223-230.
5. Case, G.L., He, L., Mo, H., Elson, C.E. 1995. Induction of geranyl pyrophosphate pyrophosphatase activity by cholesterol- suppressive isoprenoids. Lipids, 30: 357-359.
6. Cosentino, S., Tuberoso, C.I.G., Pisano, B., Satta, M., Mascia, V., Arzedi, E., Palmas, F. 1999. In vitro antimicrobial activity and composition of Sardinian Thymus essential oils. Lett. App. Microbiol., 29:130-135.
7. Craig, W.J. 1999. Health-promoting properties of common herbs. Am. J. Clin. Nut., 70 (suppl),491-499.
8. Cross, D.E., Svoboda, K., Hillman, K., Mcdevitt, R., Acamovic, T.2002. Effects of Thymus vulgaris L. Essential oil as an in vivo dietary supplement on chicken intestinal microflora. Proceedings of the 33rd International Symposium on Essential Oils, Lisbon, Portugal, 3-7th Sept.

9. Deschepper, K., Lippens, M., Huyghebaert, G., Molly, K. 2003. The effect of aromabiotic and GALI D'OR on technical performances and intestinal morphology of broilers. In: Proc. 14th European Symp. on Poultry Nutrition, August, Lillehammer, Norway, pp. 189.
10. Hammer, K.A., Carson, C.F., Riley, T.V. 1999. Antimicrobial activity of essential oils and other plant extracts. J Appl. Microbiol., 86: 985-990.
11. Hara, A., Radin, N.S. 1978. Analitical Biochemistry, 90: 420-426. Hertrampf, J.W. 2001. Alternative antibacterial performance promoters. Poult. 50-52. Int., 40:
12. Jamroz, D., Wertlecki, T.J., Orda, J., Wiliczkiewicz, A., Skorupi_ska, J. 2003. Influence of phtogenic extracts on gut microbial status in chickens. In: Proc. 14th European Symp. on Poultry Nutrition, August, Lillehammer, Norway.; pp. 176.
13. Kishimoto, K., Urade, R., Ogawa, T., Moriyama, T. 2001. Nondestructive Quantification of Neutral Lipids by Thin- Layer Chromatography and Laser- Fluorescent Scanning: Suitable Methods for “Lipidome” Analysis. Biochem. Biophys. Res. Comm., p: 657-662.
14. Lopez-Bote, L.J., Gray, J.I., Gomaa, E.A., Flegal, C.I. 1998. Effect of dietary administration of oil extracts from rosemary and sage on lipid oxidation in broiler meat. Bri. Poult. Sci., 39: 235-240.
15. Madrid, J., Hernández, F., García, V., Orengo, J., Megías, M.D., Sevilla, V. 2003. Effect of plant extracts on ileal apparent digestibility and carcass yield in broilers at level of farm. In: Proc. 14th European Symp. on Poultry Nutrition, August, Lillehammer, Norway. pp.187.
16. Moser, M., Messikommer, R., Pfirter, H.P., Wenk, C. 2003. Influence of the phytogenic feed additive sangrovit on zootechnical effects in broilers in field trials. In: Proc. 14th European Symp. On Poultry Nutrition, August, Lillehammer, Norwaypp. 205.
17. Sarica, S., Ciftci, A., Demir, E., Kılınc., K., Yıldırım, Y.2005. Use of an antibiotic growth promoter and two herbal natural feed additives with and without exogenous enzymes in wheat based broiler diets. S. Afr. J. Anim. Sci., 35: 61 -72.
18. Skipski, V. P., Barclay, M. 1969. Thin-layer chromatography of lipids. Methods Enzymol, 65: 530-598.
19. Smith-Palmer, A., Stewart, J., Fyfe, L. 1998. Antimicrobial properties of plant essential oils and essences against five important food-borne pathogens. Lett. Food Microbio., 26: 118-122.
20. SPSS 1999. SPSS for Windows Release 10.0,. SPSS Inc
21. Tucker, L.A. 2002. Plant extracts to maintain poultry performance. Feed Int., 23 : 26-29.
22. Yu, S.G., Abuirmeileh, N.M., Qureshi, A.A., Elson, C.E. 1994. Dietary _-ionone suppresses hepatic 3-hydroxy-3- methylglutaryl coenzyme A reductase activity. J. Agric. Food Chem., 42: 1493- 1496