Beta-Sitronellol ve (-)-Menton Monoterpenlerinin İnsan Meme Kanseri (MCF-7) Hücre Hattında Sitotoksik Etkilerinin İncelenmesi

Öz

Amaç: Monoterpenler, ilaç, gıda veya kozmetik endüstrisinde kullanılan, bitki esans yağlarının ana bileşenleridir. Birçok terpenoid, antialerjik, antiviral, anti-inflamatuar ve antikanser gibi çeşitli farmakolojik aktiviteler sergiler. Bununla birlikte, bu bileşiklerin insan meme kanseri (MCF-7) hücreleri üzerine sitotoksik etkileri ile ilgili yeterli çalışma bulunmamaktadır. Bu çalışmanın amacı, Beta-Sitronellol (BS) and (-)-Menton (MNT) monoterpenlerinin potansiyel sitotoksik etkilerini MCF-7 hücre hattında 3-(4,5-dimethylthiazolyl-2)-2,5 diphenyl tetrazolyum bromid (MTT) testi ile incelemektir.

Yöntem: Bu çalışmada BS ve MNT monoterpenlerinin sitotoksik etkileri, MCF-7 hücre hattında 24 ve 48 saat muamele sürelerinde MTT testi ile analiz edilmiştir. Bunun için hücrelere BS (2, 10, 50, 250, 1250 μg/mL) ve MNT (16, 80, 400, 2000, 10.000 μg/mL)’nin farklı konsantrasyonları uygulanmıştır.

Bulgular: BS ve MNT, 24 saatlik uygulama süresinde tüm konsantrasyonlarda, (%) hücre canlılığını önemli düzeyde azaltmıştır. Ayrıca 48 saatlik uygulamada, BS (250, 1250 μg/mL) ve MNT (16, 400, 2000, 10.000 μg/mL), (%) hücre canlılığını anlamlı oranda düşürmüştür.

Sonuç: Sonuç olarak; BS ve MNT, her iki muamele süresinde de MCF-7 hücreleri üzerinde sitotoksik etki göstermiştir. Ancak sitotoksisitenin belirlenmesine yönelik farklı hücre hatlarında daha fazla çalışma yapılmalıdır.

Anahtar Kelimeler

• Beta-Sitronellol,(-)-Menton,MCF-7,MTT testi

Investigation of Cytotoxic Effect of Monoterpenes Beta-Citronellol and (-)-Menthone in Human Breast Cancer (MCF-7) Cell Line

Öz

Objective: Monoterpenes are the primary components of plant essential oils which can use in medicines, food or cosmetic industries. Many terpenoids exhibit a various pharmacological activities such as antiallergic, antiviral, anti-inflammatory and anticancer. However no enough data are found about cytotoxicity of these compounds on human breast cancer (MCF-7) cells. The aim of this study was to evaluate the potential cytotoxic effects of monoterpenes Beta-Citronellol (BC) and (-)-Menthone (MNT) using 3-(4,5-dimethylthiazolyl-2)-2,5 diphenyl tetrazolium bromide (MTT) assay in MCF-7 cell line.

Methods: In this study, the cytotoxic effects of monoterpenes BC and MNT were analyzed by MTT assay at 24 and 48 hour treatment times in the MCF-7 cell line. MCF-7 cells were treated with different concentrations of BC (2, 10, 50, 250, 1250 μg/mL) and MNT (16, 80, 400, 2000, 10.000 μg/mL).

Results: BC and MNT were significantly decreased cell viability (%) at all concentrations at 24-h treatment period. Furthermore, BC (250, 1250 μg/mL) and MNT (16, 400, 2000, 10.000 μg/mL) were significantly reduced the cell viability at 48-h treatment. 

Conclusion: As a result, BC and MNT showed cytotoxic effect on MCF-7 cells in both treatment times. Further studies should be conducted to determination cytotoxicity using different cell lines. 

Anahtar Kelimeler

• Beta-Citronellol,(-)-Menthone,MCF-7,MTT assay

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