Beta-Sitronellol ve (-)-Menton Monoterpenlerinin İnsan Meme Kanseri (MCF-7) Hücre Hattında Sitotoksik Etkilerinin İncelenmesi

Yıl 2019, Cilt: 3 Sayı: 2, 111 - 119, 27.05.2019

Sevcan Mamur

Öz

Amaç: Monoterpenler, ilaç, gıda veya kozmetik endüstrisinde kullanılan,
bitki esans yağlarının ana bileşenleridir. Birçok terpenoid,
antialerjik, antiviral, anti-inflamatuar ve antikanser gibi çeşitli
farmakolojik aktiviteler sergiler. Bununla birlikte, bu bileşiklerin insan meme kanseri (MCF-7)
hücreleri üzerine sitotoksik etkileri ile ilgili yeterli çalışma
bulunmamaktadır. Bu çalışmanın amacı, Beta-Sitronellol
(BS) and (-)-Menton (MNT) monoterpenlerinin potansiyel sitotoksik etkilerini
MCF-7 hücre hattında 3-(4,5-dimethylthiazolyl-2)-2,5
diphenyl tetrazolyum bromid (MTT) testi ile incelemektir.

Yöntem: Bu çalışmada BS ve MNT monoterpenlerinin sitotoksik etkileri, MCF-7
hücre hattında 24 ve 48 saat muamele sürelerinde MTT testi ile analiz edilmiştir. Bunun için hücrelere BS (2, 10, 50, 250, 1250 μg/mL) ve MNT (16, 80, 400, 2000, 10.000 μg/mL)’nin farklı
konsantrasyonları uygulanmıştır.

Bulgular:
BS ve MNT, 24 saatlik uygulama süresinde tüm konsantrasyonlarda, (%) hücre
canlılığını önemli düzeyde azaltmıştır. Ayrıca 48 saatlik uygulamada, BS (250, 1250 μg/mL) ve MNT (16, 400, 2000, 10.000 μg/mL), (%) hücre canlılığını
anlamlı oranda düşürmüştür.

Sonuç: Sonuç olarak; BS ve MNT, her iki muamele süresinde de MCF-7
hücreleri üzerinde sitotoksik etki göstermiştir. Ancak sitotoksisitenin
belirlenmesine yönelik farklı hücre hatlarında daha fazla çalışma yapılmalıdır.

Anahtar Kelimeler

Beta-Sitronellol, (-)-Menton, MCF-7, MTT testi

Investigation of Cytotoxic Effect of Monoterpenes Beta-Citronellol and (-)-Menthone in Human Breast Cancer (MCF-7) Cell Line

Öz

Objective: Monoterpenes
are the primary components of plant essential oils which can use in medicines, food or cosmetic
industries.
Many terpenoids exhibit a various pharmacological activities such as
antiallergic, antiviral,
anti-inflammatory and anticancer. However no enough data are found about
cytotoxicity of these compounds on human breast cancer (MCF-7) cells. The aim of this study was
to evaluate the potential cytotoxic effects of monoterpenes Beta-Citronellol (BC) and (-)-Menthone (MNT) using
3-(4,5-dimethylthiazolyl-2)-2,5
diphenyl tetrazolium bromide (MTT) assay in MCF-7 cell line.

Methods: In this study, the
cytotoxic effects of monoterpenes BC and MNT were analyzed by MTT assay at 24
and 48 hour treatment times in the MCF-7 cell line. MCF-7 cells were treated with different concentrations of BC (2, 10, 50, 250, 1250 μg/mL) and MNT (16, 80, 400, 2000, 10.000 μg/mL).

Results: BC and MNT were significantly decreased cell viability (%) at
all concentrations at 24-h treatment period. Furthermore, BC (250, 1250 μg/mL) and MNT (16, 400,
2000, 10.000 μg/mL) were significantly reduced the cell viability at 48-h treatment. 

Conclusion: As a result, BC and MNT showed cytotoxic effect on MCF-7 cells in
both treatment times. Further studies
should be conducted to determination cytotoxicity using different cell lines. 

Anahtar Kelimeler

Beta-Citronellol, (-)-Menthone, MCF-7, MTT assay

Kaynakça

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