Comparison of Two Different Serological Methods in The Diagnosis of Rotavirus and Adenovirus in Stool Samples

Yıl 2024, Cilt: 8 Sayı: 3, 262 - 269, 30.09.2024

Oktay İlhan , Mehmet Tevfik Bayraktar , Bashar Ibrahim

https://doi.org/10.46237/amusbfd.1275682

Öz

Objective: Rotaviruses are the most common cause of acute infectious diarrhea in children under 5 years of age worldwide. This study aims to compare the immunochromatographic and ELISA methods, determine the sensitivity and specificity of the methods used, and shed light on humanity to diagnose the disease correctly.
Method: Stool samples of 1000 patients were sent to Diyarbakır Pediatric Hospital for rapid antigen testing of Rotavirus. Single-step Rotavirus and Adenovirus Co-immunochromatographic and ELISA methods were used in the examination of the samples. Samples with positive rapid test results were collected in 1.5 ml microcentrifuge tubes, and positive antigen samples collected for the ELISA test were stored at -80°C until the time of study.
Results: Of 1000 stool samples analyzed, 40 (11.59%) of 345 girls and 60 (9.16%) of 655 boys had Rotavirus antigen in a total of 100 (10%) and 20 (2%) had Adenovirus antigen (P> 0.05). Viral antigen-positive cases were most frequently seen in winter months (December, January, February). There was no statistically significant difference between the data in terms of age and gender (P <0.05).
Conclusion: In this study, Rotavirus was detected in one-tenth of diarrhea cases in pediatric patients and it was revealed that it increased seasonally in winter months.

Anahtar Kelimeler

Rotavirus, Gastroenteritis, ELISA, Immunochromatographic, Pediatrics

Dışkı Örneklerinde Rotavirüs ve Adinovirüs Tanısında İki Farklı Serolojik Yöntemin Karşılaştırılması

Öz

Amaç: Tüm dünyada beş yaş altındaki çocuklardaki akut enfeksiyöz ishalin en sık nedeni rotavirüslerdir. Bu çalışma immünkromatografik ve Enzyme-Linked ImmunoSorbent Assay (ELİSA) yöntemlerinin karşılaştırılması, kullanılan yöntemlerin duyarlılık ve özgüllüklerinin belirlenmesi ve hastalığın doğru teşhis edilebilmesi için insanlığa ışık tutmayı amaçlamaktadır.
Metod: Toplam 1000 hastanın Rotavirüs hızlı antijen testi çalışılması amacıyla Diyarbakır Çocuk Hastalıkları Hastanesi’ne gönderilen dışkı örnekleri çalışıldı. Örneklerin incelenmesinde tek aşamalı Rotavirüs ve Adenovirüs Birlikte immünokromatografik ve ELISA yöntemleri kullanıldı. Hızlı test sonucu pozitif olan örnekler 1.5 ml’lik mikrosantrifüj tüplerine alındı, ELISA testi için toplanan pozitif antijen numuneleri -80°C’de çalışılacağı zamana kadar saklandı. Bulgular: Analiz edilen 1000 dışkı örneğinin 345 kız çocuğunun 40' ında (%11.59) ve 655 erkek çocuğunun 60'ında (%9.16) toplam 100'ünde (%10) Rotavirüs antijeni ve 20’sinde (%2) Adenovirüs antijeni saptanmıştır (P>0,05). Viral antijen pozitif olgular en sık kış ayları (Aralık, Ocak, Şubat)’nda görüldü. Verilerin yaş ve cinsiyet yönünden istatistiksel olarak anlamlı bir farkı bulunmamıştır (P <0,05). Sonuç: Bu çalışmada çocuk hastalarındaki ishal vakalarının onda birinde Rotavirus tespit edilmiş ve kış aylarında da mevsimsel artış gösterdiği ortaya konmuştur.

Anahtar Kelimeler

Rotavirüs, Gastroenterit, ELİSA, Immünokromatografik, Pediatri

Kaynakça

• 1. Cunliffe, N. A., & Glass, R. I. (2014). Rotavirus and Other Viral Diarrhoea. Manson’s Tropical Infectious Diseases, 207–214.e3.
• 2. Ferreira, M. R., da Xavier, M. P., de Tinga, A. C., Rose, T. L., et al. (2012). Assessment of gastroenteric viruses frequency in a children’s day care center in rio de janeiro, brazil: A fifteen year study (1994-2008). PLoS One,7(3): 7: 1-7.
• 3. Parashar, U. D., Nelson, E. S., & Kang, G. (2018). Diagnosis, management, and prevention of rotavirus gastroenteritis in children. BMJ, 347: f7204.
• 4. Glass, R. I., Tate, J. E., Jiang, B., & Parashar, U. (2021). The Rotavirus Vaccine Story: From Discovery to the Eventual Control of Rotavirus Disease. Journal of Infectious Diseases, 1;224:S331–42.
• 5. Omatola, C. A., & Olaniran, A, O. (2022). Rotaviruses: From Pathogenesis to Disease Control- A Critical Review. Viruses, 14(5):875.
• 6. Crawford, S. E., Ramani, S., Tate, J. E., Parashar, U. D., et al. (2017). Rotavirus infection. Nat Rev Dis Primers, 9 (3): 17083.
• 7. Leung, A. C., & Hon, K. L. (2020). Paediatrics: How to manage viral gastroenteritis. Drugs Context, 10: 2020-11-7.
• 8. Liu, L., & Moore, M. D. (2020). A survey of analytical techniques for noroviruses. Foods, 9(3): 318.
• 9. Palacio-Mejía, L. S., Rojas-Botero, M., Molina-Vélez, D., García-Morales, C., et al. (2020). Overview of acute diarrheal disease at the dawn of the 21st century: The case of Mexico. Salud Publica Mex, 62(1):14–24.
• 10. Troeger, C., Khalil, I. A., Rao, P. C., Cao, S., et al. (2018). Rotavirus Vaccination and the Global Burden of Rotavirus Diarrhea among Children Younger Than 5 Years. JAMA Pediatr, 172(10):958–65.
• 11. Lestari, F .B., Vongpunsawad, S., Wanlapakorn, N., & Poovorawan, Y. (2020). Rotavirus infection in children in Southeast Asia 2008-2018: Disease burden, genotype distribution, seasonality, and vaccination. Journal of Biomedical Science, 27: 66.
• 12. Kahraman, H., Koçak, A. A., Albakkour, K., Muftah, H., et al. (2021). Investigation of group A rotavirus G10, G12 genotypes emerging in patients with acute gastroenteritis in a Tertiary Care Hospital. Mikrobiyol Bul, 55(4):553–67.
• 13. Kasırga, E. (2019). The importance of stool tests in diagnosis and follow-up of gastrointestinal disorders in children. Turk Pediatri Ars, 54(3): 141–148.
• 14. Özer, B., Jenedi, K., Pehlivanoğlu, C., & Göçmen, M. (2014). Rotavirus and Adenovirus Prevelance in Stool Samples of Patients with Acute Gastroenteritis. Mustafa Kemal Üniv Tıp Derg, 5 (20): 1-10.
• 15. Terzi, H. A., & Aydemir, Ö. (2018). Investigation of the Frequency of Rotavirus and Adenovirus in Patients with Acute Gastroenteritis; Sakarya. Sakarya Medical Journal, 8(4): 746 - 752.
• 16. Gentile, M, H. (2014). Electron microscopy in rapid viral diagnosis: an update. New Microbiologica, 37 403–422.
• 17. Malik, Y. S., Verma, A. K., Kumar, N., Touil, N., et al. (2019). Advances in Diagnostic Approaches for Viral Etiologies of Diarrhea: From the Lab to the Field. Frontiers in Microbiology, 10: 1957.