Efficacy of Amphotericin-B, Clinicopathologic Variables and Oxidative Stress Markers in three Staffordshire Bull Terrier Dogs with Visceral Leishmaniasis

Abstract

This study was aimed at evaluating the effects of Leishmania infection and AMB (amphotericin-B) treatment on hematological, biochemical and oxidative stress parameters in three Staffordshire Bull Terrier dogs with visceral leishmaniasis (VL). These dogs were presented with weight loss, weakness, and cutaneous lesions. Canine kala-azar detect kit was positive in dogs with VL. AMB was administered to cases with VL at a dose of 0.6 to 1.5 mg/kg/week for four months. Leishmania agents caused the liver injury in cases 1 and 2 due to increase in ALT level, decrease in erythrocyte, hemoglobin, and hematocrit level and increase in MDA, and decrease in GSH-Px in case 3 and did not affect the kidney functions due to normal urea and creatinine level in the dogs with VL. A gradual response to the administration of AMB was observed. At the end of treatment course, cases with VL were cured clinically. It was concluded that 4-month of AMB administration may be effective to treat VL due to no clinical recurrence for six months follow-up.

Keywords

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Öz

 Bu   çalışmada   visseral   Leishmaniasis’li   (VL)   üç   Staffordshire   Bull   Terrier   köpekte   Leishmania   enfeksiyonu   ve   AMB  (amfoterisin-­‐B)   tedavisinin   hematolojik,   biyokimyasal   ve   oksidatif   stres   parametreleri   üzerine   etkilerini   değerlendirmek  amaçlandı.   Bu   köpekler   kilo   kaybı,   halsizlik   ve   deri   lezyonları   ile   kliniğe   getirildi.   Kanin   kala-­‐azar   test   kiti   VL’li   köpeklerde  pozitifti.  Visseral  Leishmaniasis’li  olgulara  AMB  0.6-­‐1.5  mg/kg/hafta  dozunda  4  ay  süre  ile  uygulandı.  Leishmania  etkenleri  olgu  1  ve  2’de  ALT  düzeyinde  artışa  bağlı  olarak  karaciğer  hasarına,  olgu  3’te  eritrosit,  hemoglobin  ve  hematokrit  düzeyde  azalmaya  ve  MDA’da  artışa  ve  GSH-­‐Px’de  azalmaya  neden  oldu  fakat  VL’li  köpeklerde  normal  üre  ve  kreatinin  düzeyine  bağlı  olarak   böbrek   fonksiyonlarını   etkilemedi.   AMB   tedavisine   kademeli   yanıt   gözlendi.   Tedavi   seyrinin   sonunda   VL’li   olgular  klinik   olarak   iyileşti.   Sonuç   olarak   6   ay   süre   ile   takipte   klinik   nüks   olmamasına   bağlı   olarak   visseral   Leishmaniasis’i   tedavi  etmek  için  4  ay  süreli  AMB  uygulamasının  etkili  olabileceği  kanısına  varıldı.  

Anahtar Kelimeler

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Kaynakça

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