Ayak Hastalıklı Broilerde Antioksidan Metabolizma ve Kemik Dokusunda Oluşan Değişikliklerin Belirlenmesi

Öz

Broiler yetiştiriciliğinde önemli sorunların başında ayak problemleri gelmektedir. Bu çalışmada aynı şartlarda bakım ve beslenmesi yapılan 10 adet sağlıklı (SG) ve 10 adet ayak hastalıklı (AHG) olmak üzere toplam 20 adet broiler (Ross 308) kullanıldı. Uygun ortamda kesilen hayvanlardan alınan böbrek, kalp, karaciğer, but eti ve göğüs eti dokularındaki antioksidan metabolizmayı belirlemek için süperoksit dismutaz (SOD) ve katalaz (CAT) aktiviteleri ile glutatyon (GSH) ve lipid peroksidasyon (LPO) miktarları ölçüldü. Ayak hastalıklı grubun böbrek dokusunda CAT aktivitesinin, GSH ve LPO miktarlarının arttığı tespit edildi (P<0.05). Yine AHG’nin but dokusunda CAT aktivitesi ve LPO miktarının arttığı (P<0.01), SOD aktivitesinin ise azaldığı belirlendi (P<0.05). Göğüs dokusunda ise AHG’nin SOD aktivitesi, GSH ve LPO miktarlarının arttığı (P<0.01), CAT aktivitesinin ise azaldığı görüldü (P<0.01). Ayak Hastalıklı Grubun kalp dokusunda CAT aktivitesi ile GSH miktarının arttığı (P<0.01), SOD aktivitesinin azaldığı belirlendi (P<0.01). Ayrıca AHG’nin karaciğer dokusunda SOD aktivitesi (P<0.05) ile GSH miktarının azaldığı (P<0.05) ve LPO miktarlarının arttığı (P<0.01), CAT aktivitesinin ise değişmediği görüldü (P>0.05). Sonuç olarak; ayak hastalıklı hayvanların tibial-tarsal kemik dokusunun tam olarak şekillenmediği, böbrek, kalp, karaciğer, but ve göğüs eti dokularında antioksidan aktivitelerin önemli oranda değiştiği, ancak bu değişiminin enzimine ve dokusuna göre farklı olduğu tespit edildi.

Anahtar Kelimeler

• Antioksidan enzim
• Broiler
• Ayak hastalığı

Determination of Antioxidant Metabolism and Changes in Bone Tissue in Leg Disease Broilers

Abstract

Leg problems are one of the most important problems in broiler breeding. In this study, a total of 20 broilers (Ross 308) 10 healthy (SG) and 10 legs diseased (AHG) were used. Superoxide dismutase (SOD) and catalase (CAT) activities and glutathione (GSH) and lipid peroxidation (LPO) amounts were measured to determine the antioxidant metabolism in some tissues. The CAT activity, GSH and LPO amounts were increased in kidney tissue of the leg diseased group (P<0.05). Again, it was determined that CAT activity and LPO amount increased (P<0.01), and SOD activity decreased in drumsticks tissue of the AHG group (P<0.05). It was observed that SOD activity, GSH and LPO amounts increased (P<0.01), CAT activity decreased (P<0.01) in AHG's breast tissue. It was determined that the CAT activity and amount of GSH increased (P<0.01), and SOD activity decreased in the heart tissue of the AHG group (P<0.01). In addition, it was observed that SOD activity (P<0.05) and GSH amount decreased (P<0.05) and LPO amount increased in liver tissue of AHG (P<0.05). As a result; it was determined that the tibial-tarsal bone tissue of the foot-diseased animals was not fully formed, antioxidant activities were significantly changed in kidney, heart, liver, drumsticks and breast meat tissues.

Keywords

• Antioxidant enzyme
• Broilers
• Leg disease

Kaynakça

1. 1. Crespo R., 2020. Developmental, metabolic, and other noninfectious disorders. Editor: David E. Swayne, Diseases of Poultry, p: 1286-1329, Fourteenth edition, Hoboken, NJ: Wiley-Blackwell, ISBN 9781119371175 (ePub) | ISBN 9781119371168, John Wiley & Sons, Inc., 111 River Street, Hoboken, NJ 07030, USA. 2. Hartcher KM., Lum HK., 2020. Genetic selection of broilers and welfare consequences: a review. World Poultry Sci J, 76, 154-167. 3. Pedersen IJ., Tahamtani FM., Forkman B., Young JF., Poulsen HD., Riber, AB., 2020. Effects of environmental enrichment on health and bone characteristics of fast-growing broiler chickens. Poultry Sci, 99, 1946-1955. 4. Cao QQ., Kong AA., Tao KS., Zheng SH., Tong C., Wang XB., Huang SC., 2020. Characterization of growth performance, meat quality and serum biochemical parameters in chickens suffering from tibial dyschondroplasia. Livest Sci, 233, 103956. 5. Imik H., Terim Kapakin KA., Gümüş R., Kapakin S., Kurt A., 2012. The effect of tibial dyschondroplasia on metabolic parameters in broiler chickens. Ankara Univ Vet Fak Derg, 59, 271-277. 6. Xing R., Yang H., Wang X., Yu H., Liu S., Li P., 2020. Effects of calcium source and calcium level on growth performance, immune organ indexes, serum components, intestinal microbiota, and intestinal morphology of broiler chickens. J Appl Poultry Res, 29, 106-120. 7. Pedersen IJ., Forkman B., 2019. Improving leg health in broiler chickens: a systematic review of the effect of environmental enrichment. Anim Welfare, 28, 215-230. 8. NRC (National Research Council)., 1994. Nutrient Requirements of Poultry, 9th, Washington, DC: National Academy Press. 9. AOAC (Association of Official Analytical Chemists)., 1990. Official Methods of Analysis of the Association Official Analytical Chemists, 15th, Vol. I, Arlington, VA: AOAC. 10. Sedlak J., Lindsay RH., 1968. Estimation of total, protein-bound and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem, 25, 192-205. 11. Aebi H., 1984. Catalase in vitro. In: Methods in enzymology. Academic Press, 121-126. 12. Ohkawa H., Ohishi N., Yagi K., 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem, 95, 351-358. 13. Sun Y., Oberley LW., Li Y., 1988. A simple method for clinical assay of superoxide dismutase. Clin Chem, 34, 497-500. 14. SPSS., 2011. Statistical Packages for the Social Sciences 20th ed. IBM: Chicago. 15. Leach JrRM., Nesheim MC., 1965. Nutritional, genetic and morphological studies of an abnormal cartilage formation in young chicks. J Nutr, 86, 236-244. 16. Mehmood K., Zhang H., Iqbal MK., Rehman MU., Li K., Huang S., Shahzad M., Nabi F., Iqbal M., Li, J., 2018. Tetramethylpyrazine mitigates toxicity and liver oxidative stress in tibial dyschondroplasia chickens. Pak Vet J, 38, 76-80. 17. Kapakin KAT., Kapakin S., Imik H., Gumus R., Eser G., 2019. The investigation of the relationship between HSP-27 release and oxidative DNA damage in broiler chickens with tibial dyschondroplasia by using histopathological and immunohistochemical methods. Braz J Poultry Sci, 21, 1-6. 18. Aydın A., 2016. Bazı fizyolojik faktörlerin etlik piliçlerin bacak aksaklıkları üzerine etkisi. KSÜ Doğa Bilimleri Dergisi, 19, 249-255. 19. Puvadolpirod S., Thaxton JP., 2000. Model of physiological stress in chickens 1. Response parameters. Poultry Sci, 79, 363-369. 20. Weimer SL., Wideman RF., Scanes CG., Mauromoustakos A., Christensen KD., Vizzier-Thaxton Y., 2020. Broiler stress responses to light intensity, flooring type, and leg weakness as assessed by heterophil to lymphocyte ratios, serum corticosterone, infrared thermography, and latency to lie. Poultry Sci, 99, 3301-3311. 21. Mench JA., 1992. Welfare of poultry in modern production systems. Poultry Sci, 4, 107-128. 22. Halıcı M., İmik H., Koç M., Gümüş R., 2012. Effects of α‐lipoic acid, vitamins E and C upon the heat stress in Japanese quails. J Anim Physiol Anim Nutr, 96, 408-415