Rutin Ratlarda Metotreksata Bağlı Hepatik Oksidatif Stresi Hafifletir

Öz

Bu çalışma, rutinin ratlarda metotreksata bağlı hepatik oksidatif stres üzerindeki potansiyel antioksidan etkilerini değerlendirmeyi amaçlamıştır. Wistar Albino ratlar (n=24) dört gruba ayrıldı: Kontrol grubu (K); metotreksat (M, 20 mg/kg, tek doz) grubu; rutin (R, 50 mg/kg/gün, 15 gün) grubu; ve metotreksat + rutin [MR, metotreksat (20 mg/kg, tek doz) + rutin (50 mg/kg/gün, 15 gün)] grubu. Ratlar onaltıncı gün sakrifiye edildi. Karaciğer dokuları alındı. Malondialdehit (MDA), vitamin C, total glutatyon (tGSH), nikotinamid adenin dinükleotid fosfat (NADPH) seviyeleri ve glutatyon peroksidaz (GPx) ve glukoz-6-fosfat dehidrojenaz (G6PD) aktiviteleri kolorimetrik olarak belirlendi. M grubunda diğer gruplara göre MDA düzeyleri artmış ve C vitamini düzeyleri azalmıştır (P<0.001). tGSH düzeyleri, hem M hem de MR gruplarında K grubuna göre azaldı (P<0.05). M grubu NADPH düzeylerinde ve GPx aktivitesinde K grubuna kıyasla önemli azalma (P<0.001) gözlendi. R grubunun NADPH düzeyleri M grubuna kıyasla daha yüksek belirlendi (P<0.001). Bu çalışma, metotreksatın karaciğerde oksidatif strese neden olduğunu ve rutinin de metotreksata bağlı hepatik oksidatif stres üzerine antioksidan etkisinin olduğunu göstermektedir. Sonuç olarak rutinin metotreksatın karaciğer üzerindeki yan etkilerini hafifletmede faydalı olabileceği düşünülmektedir.

Anahtar Kelimeler

• Karaciğer
• Metotreksat
• Nikotinamid adenin dinükleotid fosfat
• Rutin
• Vitamin C

Rutin Attenuates Methotrexate-Induced Hepatic Oxidative Stress in Rats

Abstract

This study aimed to assess the antioxidant effects of rutin on methotrexate-induced hepatic oxidative stress. Wistar Albino rats (n=24) were separated into 4 groups: control group (C); methotrexate (M, 20 mg/kg, single dose) group; rutin (R, 50 mg/kg/day, for fifteen days) group; and methotrexate + rutin [MR, methotrexate (20 mg/kg, single dose) + rutin (50 mg/kg/day, for fifteen days)] group. Rats were sacrificed on the sixteenth day and liver tissues were removed. The liver glucose-6-phosphate dehydrogenase (G6PD), glutathione peroxidase (GPx), malondialdehyde (MDA), nicotinamide adenine dinucleotide phosphate (NADPH), total glutathione (tGSH), and vitamin C were determined colorimetrically. Malondialdehyde increased and vitamin C decreased in the M group compared to the others (P<0.001). Total glutathione reduced in both M and MR groups compared to C group (P<0.05). An important decrease in M group NADPH and GPx compared to C group (P<0.001) was observed. Nicotinamide adenine dinucleotide phosphate of R group were determined to be higher than M group (P<0.001). In this study, it might demonstrate that methotrexate caused oxidative stress in the liver and rutin had antioxidant effects on methotrexate-induced hepatic oxidative stress. Consequently, it can be suggested that rutin may be useful in attenuating the side effects of methotrexate on the liver.

Keywords

• Liver
• Methotrexate
• Nicotinamide adenine dinucleotide phosphate
• Rutin
• Vitamin C

Kaynakça

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