Effects of Chrysin on Carbon Tetrachloride-Induced Testicular Damage in Rats: Biochemical, Histopathological and Immunohistochemical Evaluation

Öz

The aim of this study was to evaluate the effect of chrysin on testicular damage induced by carbon tetrachloride (CCl4) in rats. Twenty-four male Spraque-Dawley rats were divided into four groups as the control group, Chrysin group, CCl4 group, and Chrysin+CCl4 group. Chrysin (100 mg/kg/body weight/day) and CCl4 (0.5 ml/kg/body weight/day in corn oil (1:1)) were administered by gavage for 14 days. Compared to the control group, it was determined that malondialdehyde (MDA) level increased, reduced glutathione (GSH) level, and superoxide dismutase (SOD) and catalase (KAT) activities decreased in the CCl4 group. It was also determined that many testicular lesions were induced, Johnsen’s Mean Testicular Biopsy Score (JMTBS) decreased, and caspase-3 immunoreactivity increased in this group. Compared to the CCl4 group, it was determined that GSH level and SOD and CAT activities increased, MDA level decreased in the Chrysin+CCl4 group. It was also observed that testicular lesions were alleviated, JMTBS increased, and caspase-3 immunoreactivity was decreased in this group. As a result, it was concluded that chrysin might have beneficial effects in the treatment of CCl4-induced testicular toxicity as a result of its antioxidant and anti-apoptotic properties.

Anahtar Kelimeler

• Apoptosis
• Carbon tetrachloride
• Chrysin
• Oxidative stress
• Testis

Ratlarda Karbon Tetraklorür Kaynaklı Testis Hasarı Üzerine Krisinin Etkileri: Biyokimyasal, Histopatolojik ve İmmunohistokimyasal Değerlendirme

Öz

Bu çalışmanın amacı, ratlarda karbon tetraklorür (CCl4) ile indüklenen testis hasarı üzerine krisinin etkisini değerlendirmektir. Yirmi dört adet erkek Spraque-Dawley rat; kontrol grubu, Krisin grubu, CCl4 grubu ve Krisin+CCl4 grubu olmak üzere dört gruba ayrıldı. Krisin (100 mg/kg/vücut ağırlığı/gün) ve CCl4 (mısır yağı (1:1) içerisinde 0.5 ml/kg/vücut ağırlığı/gün) gavaj yoluyla 14 gün boyunca uygulandı. Kontrol grubu ile karşılaştırıldığında, CCl4 grubunda; malondialdehit (MDA) düzeyinin arttığı, redükte glutatyon (GSH) düzeyi ile süperoksit dismutaz (SOD) ve katalaz (KAT) aktivitelerinin ise azaldığı belirlendi. Bu grupta ayrıca birçok testiküler lezyonun indüklendiği, Johnsen’s Ortalama Testiküler Biyopsi Skoru (JMTBS)’nun azaldığı, kaspaz-3 immünoreaktivitesinin ise arttığı belirlendi. CCl4 grubu ile karşılaştırıldığında, Krisin+CCl4 grubunda; GSH düzeyi ile SOD ve KAT aktivitelerinin arttığı, MDA düzeyinin ise azaldığı tespit edildi. Bu grupta ayrıca testiküler lezyonların hafiflediği, JMTBS’nun arttığı ve kaspaz-3 immünoreaktivitesinin ise azaldığı gözlendi. Sonuç olarak; krisinin sahip olduğu antioksidan ve anti-apoptotik özellikler neticesinde, CCl4 kaynaklı testis toksisitesinin tedavisinde faydalı etkiler gösterebileceği kanaatine varılmıştır.

Anahtar Kelimeler

• Apoptozis
• Karbon tetraklorür
• Krisin
• Oksidatif stres
• Testis

Kaynakça

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