Ergin Evcil Ördeklerin (Anas Platryhynchos) Perifer Kan Lenfositlerinde Alfa-Naftil Asetat Esteraz ve Asit Fosfataz Aktivitesinin Belirlenmesi

Yıl 2007, Cilt: 2 Sayı: 4, 122 - 128, 18.04.2010

Hasan Hüseyin Dönmez , Emrah Sur

Öz

Bu çalışma, ergin ördeklerin (Anas Platryhynchos) perifer kan lenfosit (PBL) oranı ile alfa-naftil asetat
esteraz (ANAE) ve asit fosfataz (ACP) pozitif lenfosit oranlarını belirlemek amacıyla yapıldı. Çalışmada 8
ergin evcil ördekten alınan kan örnekleri kullanıldı. ANAE-pozitif lenfosit oranları % 32.62 olarak tespit
edilirken, ACP pozitif lenfosit oranı % 78.5 olarak bulundu. Perifer kan lenfosit oranı ise % 67 olarak
belirlendi.

Anahtar Kelimeler

ACP, ANAE, lenfosit, ördek

Kaynakça

• Basso, G., Cocito, MG., Semenzato, G., Pezzutto, Cytochemical study of thymocytes and T lymphocytes. Br. J. Haematol., 44, 577- 582. L. 1980.
• Catowsky, D. 1981. Leucocyte cytochemical and immunological techniques, In “Practical Haematology”, Eds., J.V. Dacie and S.M. Lewis, 143-174, 7th edition, Churchill Livingstone.
• Çelik, İ., Aştı, RN., Ergene, N. 1991. İnsan perifer lenfositlerinin esteraz sitokimyası ve yüzey immünoglobülinlerinin immünoenzimatik yöntemle boyanarak belirlenmesi S.Ü. Tıp. Fak. Derg., 7, 4, 497-503. T ve Null
• Çelik, İ., Aştı, RN., Boyraz, MÜ. 1992. Sığır fötal perifer kan lenfositlerinin alfa-naftil asetat esteraz aktivitesi üzerinde ışık mikroskopik çalışmalar. S.Ü. Vet. Fak. Derg., 8, 2, 41-44.
• Çelik, İ., Aştı, RN., Kadak, R., Işık, MK. 1994. Farklı yaşlardaki sığırların perifer kan T- lenfosit oranlarında görülen değişiklikler. Hayvancılık Araşt. Derg., 4, 2, 68-72.
• Dönmez, HH., Çelik, İ. 1998. Erken embriyonal dönemde yumurtaya verilen testosteron propiyonat’ın tavuk bursa Fabricii’si üzerindeki etkileri. Vet. Bil. Derg., 14, 1, 119-132.
• Dönmez, N., Dönmez, H., Keskin, E., Çelik, İ. 2002. Effects of zinc supplementation to ration on some hematological parameters in broiler chicks. Biol. Trace Element Res., 87, 125-131.
• Dönmez, HH., Sur, E. 2007. Hematology and enzyme histochemistry of the peripheral blood leucocytes in Rock Partridges (Alectoris graeca), Poult. Sci., 86: in press.
• Durgun, Z., Eksen, M., Serpek, P., Keskin, E. 1990. Değişik yaş gruplarındaki yerli hibrit tavuklarda bazı hematolojik değerler. Türk Vet. Hekimliği Derg., 7,8, 11-18.
• Ergün, E., Ergün, L., Özen, A., Aştı, RN. 2004a. Determination of alpha naphthyl acetate esterase activity in the peripheral blood leukocytes of ostrich (Stuthio camelus masaicus), Revue Vét. Méd, 155, 3, 147- 150.
• Ergün, L., Özen, A., Ergün, E., Astı, RN. 2004b. Alpha naphthyl acetate esterase activity in the peripheral blood leukocytes of turkeys. Indian Vet. J., 81, 431-434. Goldberg, AF., Barka, T. 1962. Acid phosphatase activity in human blood cells. Nature, 21, 292.
• Graczyk, S. 1994. The effect of anti-bursa serum (ABS) on the intensity of acid phosphatase reaction in bursa-dependent structures of the spleen and on the level of antibodies in the blood serum. Arch. Vet. Pol., 34, 1-2,, 25-36.
• http://www.bahce.biz/hayvan/ordek.htm [Erişim: 14.11.2007]
• Hulse-Post,DJ., Sturm-Ramirez, KM.,Humberd, J., Seiler,P., Govorkova,EA., et al. 2005. Role of domestic ducks in the propagation and pathogenic H5N1 influenza viruses in Asia. Microbiol., 102,30, 10682-10687. highly
• İzci, C., Çelik, İ., Alkan, F., Oğurtan, Z., Ceylan, C., Sur, E., Özkan, Y. 2002. Histologic characteristics and local cellular immunity of the gland of third eyelid after topical ophthalmic cyclosporine for treatment of dogs with keratoconjunctivitis sicca. Am. J. Vet. Res., 63, 688-694. of 2%
• Jeurissen, SHM., Janse, EM., Kok, GL., Deboer, GF. 1989. Distribution and function of non- lymphoid cells positive for monoclonal antibody chickens and those infected with Marek’s disease Immunopathol., 22, 123-133. in healthy virus. Vet. Immunol.
• Josef, H. 2001. Blood picture and biochemistries in different species of pheasants. PhD. Thesis. Viyana Üniversitesi, Veteriner Fakültesi.
• Kajikawa, O., Koyama, H., Yashikawa, T., Tsubaki, S., Saito, H. 1983. Use of alpha- naphthyl acetate esterase staining to identify T lymphocytes in cattle. Am. J. Vet. Res., 44, 8, 1549-1552.
• Kaplow, LS., Burstone, MS. 1964. Cytochemical demonstration of acid phosphatase in haemapoietic cells in health and in various hematological disorders using azo dye techniques. J. Histochem. Cytochem., 12,2, 805-811.
• Karaca, T., Cemek, M., Kanter, M. 2006. Lipid Peroxidation and Antioxidant Levels, and Alpha Naphthyl Acetate Esterase Activity of Peripheral Blood Lymphocytes in Mallard, Muscovy and Pekin Ducks. Acta Vet. Brno., 75, 33-38.
• Konuk, T. 1981. Pratik Fizyoloji. A.Ü. Vet. Fak. Yayın., 378, A.Ü. Basımevi, Ankara.
• Koèišová, M., Stopek, D., Siroťáková, M., Rybárová, S., Jurgová, T. 1995. ACHE- positive lymphopoietic organs of pheasants after hatching. Folia Vet. 39, 3-4, 75-78. primary studies on chicken Moriya, O., phosphatase developing Histochem., 87,2, 99-105. 1989. Acid tissues of Acta embryos.
• Mueller, J., Brundel, RG., Buerki, H., Keller, HU., Hess, MW., Cottier, H. 1975. Non- specific acid esterase activity: a criterion for differentiation of T and B lymphocytes in mouse lymph nodes. Eur. J. Immunol., 5, 270-274.
• Nakanishi, H., Koyama, H., Kajikawa, O., Saito, H. 1983. Identification of bovine T and B lymphocytes in normal peripheral blood, lymph nodes and spleen. Jpn. J. Vet. Sci., 45, 1, 97-102. Osbaldiston, GW., Sulliman, RJ. 1978. Cytochemical demonstration of esterases in peripheral blood leukocytes. Am. J. Vet. Res., 39, 4, 683-685.
• Oyewale, JO. 1987. Haematological studies on apparently chickens (Gallus domesticus). Bull. Anim. Hlth. Prod. Afr., 35, 108-112. domestic
• Özcan, Z. 2005. Determination of alpha naphthyl acetate esterase activity in the peripheral blood leukocytes in Angora rabbits. Turk. J. Vet. Anim. Sci., 29, 881-884.
• Pica, A., Lodato, A., Grimaldi, MC., Della Corte, F., Galderisi, U. 1993. A study of the bone marrow precursors and hemoglobin of the blood cells of the red-legged partridge (Alectoris rufa rufa L.). Ital. J. Anat. Embryol., Oct-Dec; 98, 4, 277-292.
• Pruthi, AK., Gupta, RKP., Sadana, JR. 1987. Acid alpha naphthyl acetate esterase activity in peripheral blood lymphocytes and monocytes of chickens. J. Vet. Med. A., 34, 390-392.
• Rico, AG., Braun, JP., Benard, P., Burgat- Sacaze, V. 1977. Biometry, haematology, plasma biochemistry and plasma and tissues enzymology of the red partridge (Alectoris rufa). Ann. Rech. Vet., 8, 3, 251-256.
• Sainz, RM., Mayo, JC., Reiter, RJ., Tan, DX., Rodriguez, C. 2003. Apoptosis in primary lymphoid organs with ageing. Microsc. Res. Tech., 15; 62,6,524-539.
• SPSS, 2004. SPSS 13.0 for Windows, Copyright © SPSS Inc. Printed in USA.
• Sur, E. 2001. Yumurtaya verilen aflatoksin B1 (AF B1)’in tavukların lenfoid organlarının embriyonal gelişimi üzerindeki etkilerinin enzim araştırılması. Doktora Tezi. S.Ü. Sağlık Bilimleri Enstitüsü. yöntemlerle
• Sur, E., Çelik, İ. 2003. Effects of aflatoxin B1 on the development of the bursa of Fabricius and blood lymphocyte acid phosphatase of the chicken. Br. Poult. Sci., 44, 558-566.
• Sur, E., Çelik, İ., Öznurlu, Y., Aydın, MF., Şen, İ., histochemistry and AgNOR numbers in the peripheral blood leukocytes of 6 month-old Kangal bred Anatolian shepherd dogs. Revue Méd. Vét., 154, 10, 591-598.
• Şen, İ., Turgut, K., Çelik, İ., Kıran, MM. 2002. The importance of lymphocyte enzyme profile, inclusion bodies in circulating leukocytes conjunctival smear samples in the diagnosis on canine distemper virus infection. Indian Vet. J., 79, 213-217.
• Wulff, JC., Sale, GE., Deeg, HJ., Storb, R. 1981. Nonspesific acid esterase activity as a marker for canine T-lymphocytes. Exp. Hematol., 9, 8, 85-870.
• Yang, TJ., Jantzen, PA., Williams, LF. 1979. Acid precence of activity in bovine and human T- and B lymphocytes. Immunol., 38, 85- 93. acetate esterase:
• Yang, K., Bearman, RM., Pangalis, GA., Zelman, RJ., Rappaport, H. 1982. Acid phosphatase and alpha-naphthyl acetate esterase in neoplastic and non-neoplastic lymphocytes. Am. J. Clin. Pathol., 78,2, 141-149.
• Yörük, M., Aştı, RN., Kurtdede, N., Ağaoğlu, Z., Altunay, H. 1998. Light and electron microscopic studies on alpha-naphthyl acetate esterase activity of the peripheral blood T-lymphocytes in Van cat. Anat. Histol. Embryol., 27, 289-292.