Efficacy of Amphotericin-B, Clinicopathologic Variables and Oxidative Stress Markers in three Staffordshire Bull Terrier Dogs with Visceral Leishmaniasis

Yıl 2015, Cilt: 10 Sayı: 1, 0 - , 09.04.2015

Basak Hanedan , Mehmet Borku , Yunusemre Ozkanlar Ali Haydardedeoglu , Serkal Gazyagcı Ahmet Aydın , Seckin Ozkanlar

https://doi.org/10.17094/avbd.44294

Öz

This study was aimed at evaluating the effects of Leishmania infection and AMB (amphotericin-B) treatment on hematological, biochemical and oxidative stress parameters in three Staffordshire Bull Terrier dogs with visceral leishmaniasis (VL). These dogs were presented with weight loss, weakness, and cutaneous lesions. Canine kala-azar detect kit was positive in dogs with VL. AMB was administered to cases with VL at a dose of 0.6 to 1.5 mg/kg/week for four months. Leishmania agents caused the liver injury in cases 1 and 2 due to increase in ALT level, decrease in erythrocyte, hemoglobin, and hematocrit level and increase in MDA, and decrease in GSH-Px in case 3 and did not affect the kidney functions due to normal urea and creatinine level in the dogs with VL. A gradual response to the administration of AMB was observed. At the end of treatment course, cases with VL were cured clinically. It was concluded that 4-month of AMB administration may be effective to treat VL due to no clinical recurrence for six months follow-up.

Anahtar Kelimeler

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Kaynakça

• Abranches P., Silva-Pereira MC., Conceição-Silva FM., Santos-Gomes GM., Janz JG., 1991. Canine leishmaniasis: pathological and ecological factors influencing transmission of infection. Journal of Parasitology, 77, 557-561.
• Aktas MS., Ozkanlar S., Karakoc A., Akcay F., Ozkanlar Y., 2011. Efficacy of vitamin E + selenium and Vitamin A + D + E combinations on oxidative stress induced by long-term transportation in Holstein dairy cows. Livestock Science, 141, 76-79.
• Amusategui I., Sainz A., Rodriguez F., Tesouro MA., 2003. Distribution and relationships between clinical and biopathological parameters in canine leishmaniasis. European Journal of Epidemiology, 18, 147-156.
• Bagchi M., Mukherjee S., Basu MK., 1993. Lipid peroxidation in hepatic microsomal membranes isolated from mice in health and in experimental leishmaniasis. Indian Journal of Biochemistry & Biophysics, 30, 277-281.
• Baneth G., Shaw SE., 2002. Chemotherapy of canine leishmaniosis. Veterinary Parasitology, 106, 315-324.
• Bildik A., Kargin F., Seyrek K., Pasa S., Ozensoy S., 2004. Oxidative stress and non-enzymatic antioxidative status in dogs with visceral Leishmaniasis. Research in Veterinary Science, 77, 63-66.
• Biswas T., Pal JK., Naskar K., Ghosh DK., Ghosal J., 1995. Lipid peroxidation of erythrocytes during anemia of the hamsters infected with Leishmania donovani. Molecular and Cellular Biochemistry, 146, 99-105.
• -
• Croft SL., Coombs GH., 2003. Leishmaniasis-current chemotherapy and recent advances in the search for novel drugs. Trends in Parasitology, 19, 502-508.
• Freeman K., 2010. Update on the diagnosis and management of Leishmania spp infections in dogs in the United States. Topic in Companion Animal Medicine, 25, 149-154.
• Freitas JC., Nunes-Pinheiro DCS., Neto BEL., Santos GJLS., Amaral de Abreu CR., Braga RR., Campos RM., Fernandes de Oliveira L., 2012. Clinical and laboratory alterations in dogs naturally infected by Leishmania chagasi. Revista da Sociedade Brasileira de Medicina Tropical, 45, 24-29.
• Halliwell B., 1991. Reactive oxygen species in living systems: source, biochemistry, and role in human disease. The American Journal of Medicine, 91, 14-22.
• Heidarpour M., Soltani S., Mohri M., Khoshnegah J., 2012. Canine visceral leishmaniasis: relationships between oxidative stress, liver and kidney variables, trace elements, and clinical status. Parasitology Research, 111, 1491-1496.
• Jain SK., 1989. Hyperglicemia can cause membrane lipid peroxidation and osmotic fragility in human red blood cells. The Journal of Biological Chemistry, 264, 21340-21345.
• Lamothe J., 2001. Activity of amphotericin B in lipid emulsion in the initial treatment of canine leishmaniasis. Journal of Small Animal Practice, 42, 170-175.
• Neupane DP., Majhi S., Chandra L., Rijal S., Baral N., 2008. Erythrocyte glutathione status in human visceral leishmaniasis. Indian Journal of Clinical Biochemistry, 23, 95-97.
• Oliveira FJ., Cecchini R., 2000. Oxidative stress of liver in hamsters infected with Leishmania (L.) chagasi. Journal of Parasitology, 86, 1067-1072.
• Petersen CA., 2009. Leishmaniasis, an emerging disease found in companion animals in the United States. Topics in Companion Animal Medicine, 24, 182-188.
• Pleban PA., Munyani A., Beachum J., 1982. Determination of selenium concentration and glutathione peroxidase activity in plasma and erythrocytes. Clinical Chemistry, 28, 311-316.
• Rathore A., Jain A., Gulbake A., Shilpi S., Khare P., Jain A., Jain SK., 2011. Mannosylated liposomes bearing amphotericin B for effective management of visceral leishmaniasis. Journal of Liposome Research, 21, 333-340.
• Reis AB., Martins-Filho OA., Teixeira-Carvalho A., Carvalho MG., Mayrink W., França-Silva JC., Giunchetti RC., Genaro O., Correa-Oliveira R., 2006. Parasite density and impaired biochemical/hematological status are associated with severe clinical aspects of canine visceral leishmaniasis. Research in Veterinary Science, 81, 68-75.
• Sen G., Mukhopadhyay R., Ghosal J., Biswas T., 2001. Oxidative damage of erythrocytes: a possible mechanism for premature hemolysis in experimental visceral leishmaniasis in hamsters. Annals of Hematology, 80, 32-37.
• Toz SO., Culha G., Zeyrek FY., Ertabaklar H., Alkan MZ., Vardarlı AT., Gunduz C., Ozbel Y., 2013. A real-time ITS1-PCR based method in the diagnosis and species identification of leishmania parasite from human and dog clinical samples in Turkey. PLoS Neglected Tropical Diseases, 7, e2205.
• Wasan KM., Wasan EL., Gershkovich P., Zhu X., Tidwell RR., Werbovetz KA., Clement JG., Thornton SJ., 2009. Highly effective oral amphotericin B formulation against murine visceral leishmaniasis. The Journal of Infectious Diseases, 200, 357-360.