First Detection of Lake Sinai Virus (LSV) in Varroa Mites in Turkey

Yıl 2022, Cilt 17, Sayı 1, 16 - 19, 29.04.2022

Abdurrahman Anıl ÇAĞIRGAN Murat KAPLAN Kemal PEKMEZ Fatih ARSLAN

Öz

Bee viruses are one of the most important agents that negatively affect colony health. It is known that viruses are generally associated with colony losses. LSV, one of the viruses that affect colony health, is an RNA virus that was first detected in honey bees in 2009 in the United States of America and has not yet been classified. Although its pathogenicity is not fully known yet, it is known to affect colony health. Varroa mites affect the health of the colony negatively by transmitting viruses to honey bees both biologically and mechanically. This study, it was aimed to molecularly detect the LSV genome by RT-PCR in Varroa mites, which play a vector role in the transmission of bee viruses to honey bees. LSV genome was positive in 12 of the Varroa mites collected from 26 different apiaries in İzmir (n = 6) and Muğla (n = 20). 50% of Varroa samples collected from Muğla province and 33.3% of samples collected from İzmir province were LSV positive. In conclusion, in this study, the LSV viral genome was detected for the first time in the country, and it tried to expand the knowledge about the diversity of viral diseases of honey bees in Turkey and Varroa mites, which play a biological or mechanical role in the transmission of viral diseases. Further studies are needed to determine the association of LSV with Varroa mites and the actual impact on honey bee populations in the country.

Anahtar Kelimeler

Honey bee, Lake sinai virus, Turkey, Varroa mite

Türkiye’de Varroa Akarlarında Lake Sinai Virus (LSV)’ un İlk Tespiti

Öz

Arı virusları koloni sağlığını olumsuz yönde etkileyen en önemli faktörlerdendir. Virusların genellikle koloni kayıplarıyla bağlantılı olduğu bilinmektedir. Koloni sağlığını etkileyen viruslardan olan LSV, bal arılarında ilk kez 2009 yılında Amerika Birleşik Devletleri (ABD)’ nde keşfedilmiş henüz sınıflandıralamamış bir RNA virusudur. Patojenitesi henüz tam olarak bilinmemesine rağmen koloni sağlığını etkilediği bilinmektedir. Varroa akarları ise virusları bal arılarına hem biyolojik hem de mekanik olarak bulaştırarak koloni sağlığını olumsuz etkilemektedir. Bu çalışmada, arı viruslarının bal arılarına bulaşmasında vektör rolü oynayan Varroa akarlarında LSV genomunun RT-PCR ile moleküler olarak tespiti amaçlanmıştır. İzmir (n = 6) ve Muğla (n = 20) illerinde yer alan birbirinden farklı 26 arılıktan toplanan Varroa akarlarının 12’sinde LSV genomu pozitif olarak tespit edilmiştir. Muğla ilinden toplanan Varroa örneklerinin %50’si, İzmir ilinden toplanan örneklerin ise %33,3’ü LSV pozitiftir. Sonuç olarak, bu çalışmada LSV genomu ülkede ilk defa tespit edilmiş, Türkiye'de bulunan bal arılarının viral hastalıklarının bulaşmasında biyolojik veya mekanik vektörlük yapan Varroa akarları hakkındaki bilgileri genişletmeye çalışmıştır. LSV’nin Varroa akarları ile ilişkisini ve ülkedeki bal arılarının populasyonları üzerindeki gerçek etkisini belirlemek için daha ileri çalışmalara ihtiyaç duyulmaktadır.

Anahtar Kelimeler

bal arısı, Lake Sinai Virus, Türkiye, Varroa akarı

Kaynakça

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