Araştırma Makalesi
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Chemical Components, Antimicrobial and Antioxidant Activities of Nest Materials Vespa crabro germana Christ, 1791 (Hymenoptera: Vespidae)

Yıl 2020, Cilt 51, Sayı 1, 44 - 52, 25.01.2020
https://doi.org/10.17097/ataunizfd.593016

Öz

Social wasps build their nests in nature by using various organic and inorganic materials. In this study, the total phenolic content, antioxidant potentials based on ferric reducing antioxidant power (FRAP) and DPPH radical scavenging activity, biochemical composition and antimicrobial activity of the ethanol extract of Vespa crabro germana Christ, 1791 nest were investigated. Antibacterial and antifungal activity was determined by disc diffusion method against 8 bacteria and 2 fungi species. The ethanol extract of the investigated nest sample showed maximum antimicrobial activities against Proteus vulgaris, Bacillus subtillis, Micrococcus luteus and Candida albicans. On the other hand, the minimum activities obtained against Pseudomonas aeruginosa, Yersinia enterocolitica, Aspergillus niger and Klebsiella pneumoniae. Total phenolic content (TPC) of sample extract was analyzed using Folin–Ciocalteu assay and calculated as 0.56 mg gallic acid equivalent/g sample. FRAP value and DPPH radical scavenging activity of the extract value were determined as 1.94 mg trolox equivalent/g sample and 0.88 mg trolox equivalent/g sample, respectively. GC-MS analysis of the sample was according to solid phase microextraction (SPME) technique and 44 compounds were identified. The presence of these compounds with bioactive potential, add value to investigated sample extract with a pharmaceutical meaning as antifungal, antibacterial, antiviral agents and so on. In other words, the antioxidative and antimicrobial potantial of the nest sample could be attributed to presence of these compounds. For this reason, the materials used in the structure of the nests used for breeding by the social wasp are important for the biological activities.

Kaynakça

  • Akyüz, E., Şahin, H., Islamoglu, F., Kolayli, S., Sandra, P., 2014. Evaluation of phenolic compounds in Tilia rubra subsp. caucasica by HPLC-UV and HPLC-UV-MS/MS. International Journal of Food Properties., 17(2): 331-343.
  • Anderson, K.E., Sheehan, T.H., Eckholm, B.J., Mott, B.M., DeGrandi-Hoffman, G. 2011. An emerging paradigm of colony health: microbial balance of the honey bee and hive (Apis mellifera). Insectes Sociaux., 58 (4): 431.
  • Bagriacik, N., 2011. Determination of some structural features of the nest paper of Vespa orientalis Linneaus, 1771 and Vespa crabro Linneaus, 1758 (Hymenoptera: Vespinae) in Turkey. Archives of Biological Sciences., 63(2): 449-455.
  • Banks, B.E., Shipolini, R.A., 1986. Chemistry and pharmacology of honey-bee venom. Venoms of the Hymenoptera: Biochemical, pharmacological and behavioural aspects., 330-416.
  • Benzie, I.F., Strain, J.J., 1996. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical biochemistry., 239(1): 70-76.
  • Blum, M.S., Walker, J.R., Callahan, P.S., & Novak, A.F., 1958. Chemical, insecticidal, and antibiotic properties of fire ant venom. Science., 128(3319): 306-307.
  • Christe, P., Oppliger, A., Bancalà, F., Castella, G., Chapuisat, M., 2003. Evidence for collective medication in ants. Ecology Letters., 6(1): 19-22.
  • Ertürk, Ö., Kati, H., Yayli, N., Demirbağ, Z., 2003. Antimicrobial activity of Viscum album L. subsp. abietis (Wiesb). Turkish Journal of Biology., 27(4): 255-258.
  • Ertürk, Ö., Karakaş, F.P., Pehlivan, D., Nas, N., 2009. The antibacterial and antifungal effects of Rhododendron derived mad honey and extracts of four Rhododendron species. Turkish Journal of Biology., 33(2): 151-158.
  • Jeanne, R.L., 1977. Ultimate factors in social wasp nesting behavior. Proceedings of the International Congress of the International Union for the Study of Social Insects., 164-168.
  • Jeanne, R.L., 1986. The organization of work in Polybia occidentalis: costs and benefits of specialization in a social wasp. Behavioral Ecology and Sociobiology., 19(5): 333-341.
  • Jeanne, R.L., 1996. The evolution of exocrine gland function in wasps. Natural History and Evolution of Paper Wasps., 145-160.
  • Langenheim, J.H., 2003. Plant resins: chemistry, evolution, ecology, and ethnobotany (No. 620.1924 L275p). Oregon, US: Timber Press.
  • Lokvam, J., Braddock, J.F., 1999. Anti-bacterial function in the sexually dimorphic pollinator rewards of Clusia grandiflora (Clusiaceae). Oecologia., 119(4): 534-540.
  • Maschwitz, U., Dorow, W.H.O., Botz, T., 1990. Chemical composition of the nest walls, and nesting behaviour, of Ropalidia (Icarielia) opifex van der Vecht, 1962 (Hymenoptera: Vespidae), a Southeast Asian social wasp with translucent nests. Journal of Natural History., 24(5): 1311-1319.
  • McGovern, J.N., Jeanne, R.L., Effland, M.J., 1988. The nature of wasp nest paper. Tappi Journal., 71: 133-139.
  • Monteiro, M.C., Romão, P.R., Soare, A.M., 2009. Pharmacological perspectives of wasp venom. Protein and peptide letters., 16(8): 944-952.
  • Ronald, M.A., 1990. Microbiologia. Compania editorial continental SA de CV, Mexico, DF, 505.
  • Rosengaus, R.B., Guldin, M.R., Traniello, J.F., 1998. Inhibitory effect of termite fecal pellets on fungal spore germination. Journal of Chemical Ecology., 24(10): 1697-1706.
  • Rukaiyat, M., Garba, S., Labaran, S., 2015. Antimicrobial activities of hexacosane isolated from Sanseveria liberica (Gerome and Labroy) plant. Adv Med Plant Res., 3(3): 120-125.
  • Schmid-Hempel, P., 1998. Parasites in social insects. Princeton University Press.
  • Schremmer, F., Marz, L., Simonsberger, P., 1985. Chitin im Speichel der Papierwespen (soziale Faltenwespen, Vespidae): Biologie, Chemismus, Feinstruktur. Mikroskopie., 42(1-2): 52-56.
  • Silva, T.M.S., Camara, C.A., da Silva Lins, A.C., Barbosa-Filho, J.M., da Silva, E.M.S., Freitas, B.M., dos Santos, F.D.A.R., 2006. Chemical composition and free radical scavenging activity of pollen loads from stingless bee Melipona subnitida Ducke. Journal of food composition and analysis., 19(6-7): 507-511.
  • Singleton, V.L., Rossi, J.A., 1965. Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. Am J Enol Viticult., 16: 144-158.
  • Smania, E.F.A., Monoache, F.D., Yunes, R.A., Pauler, R., Junior, A.S., 2007. Antimicrobial activity of methyl australate from Ganoderma australe. Brazilian Journal of Pharmacognosy., 17(1): 14-16.
  • Spradbery, J.P., 1973. Wasps. An account of the biology and natural history of social and solitary wasps, with particular reference to those of the British Isles.
  • Starr, C.K., 1991. The nest as the locus of social life. The social biology of wasps., 520-539.
  • Subbaiyan, B., Samydurai, P., KarthikPrabu, M., Thangapandian, V., 2014. Gas chromatography and mass spectrum analysis of Catharanthus pusillus (Murray) g. Don (Apocyanaceae). International Research Journal of Pharmaceutical and Applied Sciences (IRJPAS)., 4(2): 4.
  • Tamil Muthu, P., Selvaraj, D., 2015. Analysis of Bioactive Constituents from the Flesh of Turbo brunneus (Roding, 1798) by GCMS. International Journal of Fisheries and Aquatic Studies., 3(1): 257-259.
  • Yamane, S., Kudô, K., Tajima, T., Nihon’yanagi, K., Shinoda, M., Saito, K., Yamamoto, H., 1998. Comparison of investment in nest construction by the foundresses of consubgeneric Polistes wasps, P. (polistes) riparius and P. chinensis (Hymenoptera: Vespidae). Journal of Ethology., 16(2): 97-104.
  • Yıldırım, E., Özbek, H., 1992. Türkiye Vespinae (Hymenoptera: Vespoidea: Vespidae) türleri üzerinde sistematik ve faunistik çalışmalar. Türk Entomoloji Derg., 16(4): 227-242.

Vespa crabro germana Christ, 1791 (Hymenoptera: Vespidae)’in Yuva Materyalinin Kimyasal Bileşenleri, Antimikrobiyal ve Antioksidan Aktiviteleri

Yıl 2020, Cilt 51, Sayı 1, 44 - 52, 25.01.2020
https://doi.org/10.17097/ataunizfd.593016

Öz

Sosyal eşekarısı, yuvalarını doğada çeşitli organik ve inorganik malzemeler kullanarak oluşturur. Bu çalışmada, Vespa crabro germana Christ, 1791 yuvasına ait etanol ekstresinin toplam fenolik madde (TFM) içeriği, ferrik iyon indirgeyici antioksidan gücü (FRAP) ve DPPH radikal süpürme etkisine dayalı antioksidan potansiyeli, biyokimyasal bileşimi ve antimikrobiyal aktivitesi araştırılmıştır. Disk difüzyon yöntemi kullanılarak 8 bakteri ve 2 mantar türüne karşı antibakteriyel ve antifungal aktivite belirlenmiştir. İncelenen yuva numunesinin etanol ekstresi, Proteus vulgaris, Bacillus subtillis, Micrococcus luteus ve Candida albicans'a karşı maksimum antimikrobiyal aktivite göstermiştir. Öte yandan, Pseudomonas aeruginosa, Yersinia enterocolitica, Aspergillus niger ve Klebsiella pneumoniae'ye karşı minimum aktivite elde edilmiştir. Örnek ekstraktının TFM içeriği Folin-Ciocalteu testi kullanılarak analiz edilmiş ve 0.56 mg gallik asit eşdeğeri (GAE)/g numune olarak hesaplanmıştır. Ekstrenin FRAP değeri ve DPPH radikal süpürme aktivitesi sırasıyla 1.94 mg troloks eşdeğeri (TE)/numune ve 0.88 mg TE/g numune olarak tespit edilmiştir. Numunenin GC-MS analizi katı faz mikroekstraksiyon (SPME) tekniğine göre yapılmış ve 44 bileşik tanımlanmıştır. Biyoaktif potansiyeli olan bu bileşiklerin varlığı, araştırılan numune ekstraktına, antifungal, antibakteriyel, antiviral ajanlar vb. gibi farmasötik anlamda katma değer sağlar. Başka bir ifadeyle, yuva numunesinin antioksidan ve antimikrobiyal potansiyeli, bu bileşiklerin varlığına atfedilebilir. Bu nedenle sosyal eşekarısı tarafından yavru yetiştiriciliği için kullanılan yuvaların yapısında kullanılan malzemeler  biyolojik aktiviteler açısından önem arz eder.

Kaynakça

  • Akyüz, E., Şahin, H., Islamoglu, F., Kolayli, S., Sandra, P., 2014. Evaluation of phenolic compounds in Tilia rubra subsp. caucasica by HPLC-UV and HPLC-UV-MS/MS. International Journal of Food Properties., 17(2): 331-343.
  • Anderson, K.E., Sheehan, T.H., Eckholm, B.J., Mott, B.M., DeGrandi-Hoffman, G. 2011. An emerging paradigm of colony health: microbial balance of the honey bee and hive (Apis mellifera). Insectes Sociaux., 58 (4): 431.
  • Bagriacik, N., 2011. Determination of some structural features of the nest paper of Vespa orientalis Linneaus, 1771 and Vespa crabro Linneaus, 1758 (Hymenoptera: Vespinae) in Turkey. Archives of Biological Sciences., 63(2): 449-455.
  • Banks, B.E., Shipolini, R.A., 1986. Chemistry and pharmacology of honey-bee venom. Venoms of the Hymenoptera: Biochemical, pharmacological and behavioural aspects., 330-416.
  • Benzie, I.F., Strain, J.J., 1996. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical biochemistry., 239(1): 70-76.
  • Blum, M.S., Walker, J.R., Callahan, P.S., & Novak, A.F., 1958. Chemical, insecticidal, and antibiotic properties of fire ant venom. Science., 128(3319): 306-307.
  • Christe, P., Oppliger, A., Bancalà, F., Castella, G., Chapuisat, M., 2003. Evidence for collective medication in ants. Ecology Letters., 6(1): 19-22.
  • Ertürk, Ö., Kati, H., Yayli, N., Demirbağ, Z., 2003. Antimicrobial activity of Viscum album L. subsp. abietis (Wiesb). Turkish Journal of Biology., 27(4): 255-258.
  • Ertürk, Ö., Karakaş, F.P., Pehlivan, D., Nas, N., 2009. The antibacterial and antifungal effects of Rhododendron derived mad honey and extracts of four Rhododendron species. Turkish Journal of Biology., 33(2): 151-158.
  • Jeanne, R.L., 1977. Ultimate factors in social wasp nesting behavior. Proceedings of the International Congress of the International Union for the Study of Social Insects., 164-168.
  • Jeanne, R.L., 1986. The organization of work in Polybia occidentalis: costs and benefits of specialization in a social wasp. Behavioral Ecology and Sociobiology., 19(5): 333-341.
  • Jeanne, R.L., 1996. The evolution of exocrine gland function in wasps. Natural History and Evolution of Paper Wasps., 145-160.
  • Langenheim, J.H., 2003. Plant resins: chemistry, evolution, ecology, and ethnobotany (No. 620.1924 L275p). Oregon, US: Timber Press.
  • Lokvam, J., Braddock, J.F., 1999. Anti-bacterial function in the sexually dimorphic pollinator rewards of Clusia grandiflora (Clusiaceae). Oecologia., 119(4): 534-540.
  • Maschwitz, U., Dorow, W.H.O., Botz, T., 1990. Chemical composition of the nest walls, and nesting behaviour, of Ropalidia (Icarielia) opifex van der Vecht, 1962 (Hymenoptera: Vespidae), a Southeast Asian social wasp with translucent nests. Journal of Natural History., 24(5): 1311-1319.
  • McGovern, J.N., Jeanne, R.L., Effland, M.J., 1988. The nature of wasp nest paper. Tappi Journal., 71: 133-139.
  • Monteiro, M.C., Romão, P.R., Soare, A.M., 2009. Pharmacological perspectives of wasp venom. Protein and peptide letters., 16(8): 944-952.
  • Ronald, M.A., 1990. Microbiologia. Compania editorial continental SA de CV, Mexico, DF, 505.
  • Rosengaus, R.B., Guldin, M.R., Traniello, J.F., 1998. Inhibitory effect of termite fecal pellets on fungal spore germination. Journal of Chemical Ecology., 24(10): 1697-1706.
  • Rukaiyat, M., Garba, S., Labaran, S., 2015. Antimicrobial activities of hexacosane isolated from Sanseveria liberica (Gerome and Labroy) plant. Adv Med Plant Res., 3(3): 120-125.
  • Schmid-Hempel, P., 1998. Parasites in social insects. Princeton University Press.
  • Schremmer, F., Marz, L., Simonsberger, P., 1985. Chitin im Speichel der Papierwespen (soziale Faltenwespen, Vespidae): Biologie, Chemismus, Feinstruktur. Mikroskopie., 42(1-2): 52-56.
  • Silva, T.M.S., Camara, C.A., da Silva Lins, A.C., Barbosa-Filho, J.M., da Silva, E.M.S., Freitas, B.M., dos Santos, F.D.A.R., 2006. Chemical composition and free radical scavenging activity of pollen loads from stingless bee Melipona subnitida Ducke. Journal of food composition and analysis., 19(6-7): 507-511.
  • Singleton, V.L., Rossi, J.A., 1965. Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. Am J Enol Viticult., 16: 144-158.
  • Smania, E.F.A., Monoache, F.D., Yunes, R.A., Pauler, R., Junior, A.S., 2007. Antimicrobial activity of methyl australate from Ganoderma australe. Brazilian Journal of Pharmacognosy., 17(1): 14-16.
  • Spradbery, J.P., 1973. Wasps. An account of the biology and natural history of social and solitary wasps, with particular reference to those of the British Isles.
  • Starr, C.K., 1991. The nest as the locus of social life. The social biology of wasps., 520-539.
  • Subbaiyan, B., Samydurai, P., KarthikPrabu, M., Thangapandian, V., 2014. Gas chromatography and mass spectrum analysis of Catharanthus pusillus (Murray) g. Don (Apocyanaceae). International Research Journal of Pharmaceutical and Applied Sciences (IRJPAS)., 4(2): 4.
  • Tamil Muthu, P., Selvaraj, D., 2015. Analysis of Bioactive Constituents from the Flesh of Turbo brunneus (Roding, 1798) by GCMS. International Journal of Fisheries and Aquatic Studies., 3(1): 257-259.
  • Yamane, S., Kudô, K., Tajima, T., Nihon’yanagi, K., Shinoda, M., Saito, K., Yamamoto, H., 1998. Comparison of investment in nest construction by the foundresses of consubgeneric Polistes wasps, P. (polistes) riparius and P. chinensis (Hymenoptera: Vespidae). Journal of Ethology., 16(2): 97-104.
  • Yıldırım, E., Özbek, H., 1992. Türkiye Vespinae (Hymenoptera: Vespoidea: Vespidae) türleri üzerinde sistematik ve faunistik çalışmalar. Türk Entomoloji Derg., 16(4): 227-242.

Ayrıntılar

Birincil Dil Türkçe
Konular Fen
Bölüm ARAŞTIRMALAR
Yazarlar

Ömer ERTÜRK (Sorumlu Yazar)
ORDU ÜNİVERSİTESİ
0000-0001-5837-6893
Türkiye


Atilla ŞİMŞEK Bu kişi benim
ORDU ÜNİVERSİTESİ
0000-0003-2092-1803
Türkiye

Yayımlanma Tarihi 25 Ocak 2020
Yayınlandığı Sayı Yıl 2020, Cilt 51, Sayı 1

Kaynak Göster

Bibtex @araştırma makalesi { ataunizfd593016, journal = {Atatürk Üniversitesi Ziraat Fakültesi Dergisi}, issn = {1300-9036}, eissn = {2651-5016}, address = {}, publisher = {Atatürk Üniversitesi}, year = {2020}, volume = {51}, number = {1}, pages = {44 - 52}, doi = {10.17097/ataunizfd.593016}, title = {Vespa crabro germana Christ, 1791 (Hymenoptera: Vespidae)’in Yuva Materyalinin Kimyasal Bileşenleri, Antimikrobiyal ve Antioksidan Aktiviteleri}, key = {cite}, author = {Ertürk, Ömer and Şimşek, Atilla} }
APA Ertürk, Ö. & Şimşek, A. (2020). Vespa crabro germana Christ, 1791 (Hymenoptera: Vespidae)’in Yuva Materyalinin Kimyasal Bileşenleri, Antimikrobiyal ve Antioksidan Aktiviteleri . Atatürk Üniversitesi Ziraat Fakültesi Dergisi , 51 (1) , 44-52 . DOI: 10.17097/ataunizfd.593016
MLA Ertürk, Ö. , Şimşek, A. "Vespa crabro germana Christ, 1791 (Hymenoptera: Vespidae)’in Yuva Materyalinin Kimyasal Bileşenleri, Antimikrobiyal ve Antioksidan Aktiviteleri" . Atatürk Üniversitesi Ziraat Fakültesi Dergisi 51 (2020 ): 44-52 <https://dergipark.org.tr/tr/pub/ataunizfd/issue/52065/593016>
Chicago Ertürk, Ö. , Şimşek, A. "Vespa crabro germana Christ, 1791 (Hymenoptera: Vespidae)’in Yuva Materyalinin Kimyasal Bileşenleri, Antimikrobiyal ve Antioksidan Aktiviteleri". Atatürk Üniversitesi Ziraat Fakültesi Dergisi 51 (2020 ): 44-52
RIS TY - JOUR T1 - Vespa crabro germana Christ, 1791 (Hymenoptera: Vespidae)’in Yuva Materyalinin Kimyasal Bileşenleri, Antimikrobiyal ve Antioksidan Aktiviteleri AU - Ömer Ertürk , Atilla Şimşek Y1 - 2020 PY - 2020 N1 - doi: 10.17097/ataunizfd.593016 DO - 10.17097/ataunizfd.593016 T2 - Atatürk Üniversitesi Ziraat Fakültesi Dergisi JF - Journal JO - JOR SP - 44 EP - 52 VL - 51 IS - 1 SN - 1300-9036-2651-5016 M3 - doi: 10.17097/ataunizfd.593016 UR - https://doi.org/10.17097/ataunizfd.593016 Y2 - 2019 ER -
EndNote %0 Atatürk Üniversitesi Ziraat Fakültesi Dergisi Vespa crabro germana Christ, 1791 (Hymenoptera: Vespidae)’in Yuva Materyalinin Kimyasal Bileşenleri, Antimikrobiyal ve Antioksidan Aktiviteleri %A Ömer Ertürk , Atilla Şimşek %T Vespa crabro germana Christ, 1791 (Hymenoptera: Vespidae)’in Yuva Materyalinin Kimyasal Bileşenleri, Antimikrobiyal ve Antioksidan Aktiviteleri %D 2020 %J Atatürk Üniversitesi Ziraat Fakültesi Dergisi %P 1300-9036-2651-5016 %V 51 %N 1 %R doi: 10.17097/ataunizfd.593016 %U 10.17097/ataunizfd.593016
ISNAD Ertürk, Ömer , Şimşek, Atilla . "Vespa crabro germana Christ, 1791 (Hymenoptera: Vespidae)’in Yuva Materyalinin Kimyasal Bileşenleri, Antimikrobiyal ve Antioksidan Aktiviteleri". Atatürk Üniversitesi Ziraat Fakültesi Dergisi 51 / 1 (Ocak 2020): 44-52 . https://doi.org/10.17097/ataunizfd.593016
AMA Ertürk Ö. , Şimşek A. Vespa crabro germana Christ, 1791 (Hymenoptera: Vespidae)’in Yuva Materyalinin Kimyasal Bileşenleri, Antimikrobiyal ve Antioksidan Aktiviteleri. Atatürk Üniversitesi Ziraat Fakültesi Dergisi. 2020; 51(1): 44-52.
Vancouver Ertürk Ö. , Şimşek A. Vespa crabro germana Christ, 1791 (Hymenoptera: Vespidae)’in Yuva Materyalinin Kimyasal Bileşenleri, Antimikrobiyal ve Antioksidan Aktiviteleri. Atatürk Üniversitesi Ziraat Fakültesi Dergisi. 2020; 51(1): 44-52.
IEEE Ö. Ertürk ve A. Şimşek , "Vespa crabro germana Christ, 1791 (Hymenoptera: Vespidae)’in Yuva Materyalinin Kimyasal Bileşenleri, Antimikrobiyal ve Antioksidan Aktiviteleri", Atatürk Üniversitesi Ziraat Fakültesi Dergisi, c. 51, sayı. 1, ss. 44-52, Oca. 2020, doi:10.17097/ataunizfd.593016

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