Investigation of the biocompatibility and in vivo wound healing effect of Cotinus coggygria extracts

Abstract

Cotinus coggygria is widely recognized its antiseptic, anti-inflammatory, antimicrobial, antihemorrhagic, and wound-healing properties. In this, aimed to evaluate the phenolic contents, cytotoxicity/proliferation, hemolytic, antimicrobial, genotoxic, apoptotic, necrotic activities, and in vivo wound healing effects of C. coggygria, a plant species known to have beneficial effects on wound healing. TOF-LC/MS analyzes revealed that the methanol extract of C. coggygria leaves contained flavonoids and phenolic compounds such as gallic acid (18.5 mg/kg), catechin (4.6 mg/kg), protocatesic acid (0.6 mg/kg), vanillic acid (8.4 mg/kg), ellagic acid (0.1 mg/kg), rosmarinic acid (0.1 mg/kg), quercetin (15 ppb) and C. coggygria stems contained such as gallic acid (24.6 mg/kg), catechin (155.1 mg/kg), chlorogenic acid (1.9 mg/kg), 4-hydroxybenzoic acid (383.3 mg/kg), rutin (2.5 mg/kg), ellagic acid (15.1 mg/kg), apigenin 7-glycoside (10.5 mg/kg), rosmarinic acid (0,4 mg/kg), quercetin (15.2 mg/kg), naringenin (279.1 mg/kg). Consequently, C. coggyria has a positive effect on wound healing with antibacterial properties, particularly against E. coli, and without cytotoxic, genotoxic, or hemolytic effects at test concentrations. In the in vivo burn model, wounds treated with leaf and stem extracts healed faster than the control group. Thus, C. coggygria is an effective plant for wound healing with antibacterial properties, particularly against E. coli, and without cytotoxic, genotoxic, and hemolytic effects.

Keywords

• cytotoxicity
• Cotinus coggyria
• genotoxicity
• hemolysis
• In vivo wound healing

References

1. Aksoy H, Sancar M, Sen A, et al (2016): The effect of topical ethanol extract of Cotinus coggygria Scop. on cutaneous wound healing in rats. Nat Prod Res, 30, 452-455.
2. Aksoy H, Sen A, Sancar M, et al (2016): Ethanol extract of Cotinus coggygria leaves accelerates wound healing process in diabetic rats. Pharm Biol, 54, 2732-2736.
3. Alam G, Singh MP, Singh A (2011): Wound healing potential of some medicinal plants. Int J Pharm Sci Rev Res, 9, 136-145.
4. Artun T, Karagoz A, Ozcan G, et al (2016): In vitro anticancer and cytotoxic activities of some plant extracts on HeLa and Vero cell lines. J BUON, 21, 720-725.
5. Astm F (2000): 756-00. Standard practice for assessment of hemolytic properties of materials. ASTM.
6. Bhat SH, Ullah MF, Abu-Duhier FM (2019): Anti-hemolytic activity and antioxidant studies of Caralluma quadrangula: potential for nutraceutical development in cancers and blood disorders. Int J Pharm Res Allied Sci, 8, 121-129.
7. Bozkaya O, Arat E, Gök ZG, et al (2022): Production and characterization of hybrid nanofiber wound dressing containing Centella asiatica coated silver nanoparticles by mutual electrospinning method. Eur Polym J, 166, 111023.
8. Budovsky A, Yarmolinsky L, Ben‐Shabat S (2015): Effect of medicinal plants on wound healing. Wound Repair Regen, 23, 171-183.

9. Ciftci H, Türk M, Tamer U, et al (2013): Silver nanoparticles: cytotoxic, apoptotic, and necrotic effects on MCF-7 cells. Turk J Biol, 37, 573-581.
10. Demirci B, Demirci F, Başer KHC (2003): Composition of the essential oil of Cotinus coggygria Scop. from Turkey. Flavour Fragr J, 18, 43-44.
11. Dülger B, Hacıoglu N, Bilen S (2009): Antimicrobial activity of Cotinus coggyria from Turkey. Asian J Chem, 21, 4139-4140.
12. Eck-Varanka B, Kováts N, Horváth E (2019): Assessing Genotoxic Potential of Cofinus Coggygria Scop: Using the Mussel Micronucleus Test. J Bot Res, 2, 57-59.
13. Erenler R, Telci I, Ulutas M, et al (2015): Chemical Constituents, Quantitative Analysis and Antioxidant Activities of E chinacea purpurea (L.) M oench and E chinacea pallida (N utt.) N utt. J Food Biochem, 39, 622-630.
14. Ertaş B, Okuyan B, Şen A, et al (2022): The effect of Cotinus coggygria L. ethanol extract in the treatment of burn wounds. J Res Pharm, 26, 554-564.
15. Farahpour MR (2019): Medicinal plants in wound healing. Wound Heal Curr Perspect, 2019, 33-47.
16. Ferrazzano GF, Roberto L, Catania MR, et al (2013): Screening and scoring of antimicrobial and biological activities of Italian vulnerary plants against major oral pathogenic bacteria. Evid Based Complementary Altern Med, 2013.
17. Firdous SM, Sautya D (2018): Medicinal plants with wound healing potential. Bangladesh J Pharmacol, 13, 41-52.
18. Gizaw M, Thompson J, Faglie A, et al (2018): Electrospun fibers as a dressing material for drug and biological agent delivery in wound healing applications. Bioeng, 5, 9.
19. Hemmati A, Rezaie A, Tamri P, et al (2017): Evaluation of the healing effects of Onosma bolbutrichum root extract on second‎ degree‎ burn wound in rabbit. J Appl Pharm Sci, 7, 168-171.
20. Iliev I, Ivanov I, Todorova K, et al (2020): Effects of a Cotinus coggygria ethyl acetate extract on two human normal cell lines. Acta Morphol Anthropol, 27, 25-29.
21. Kantar C, Mavi V, Baltaş N, et al (2016): Novel zinc (II) phthalocyanines bearing azo-containing schiff base: Determination of pKa values, absorption, emission, enzyme inhibition and photochemical properties. J Mol Struct, 1122, 88-99.
22. Kumarasamyraja D, Jeganathan N, Manavalan R (2012): A review on medicinal plants with potential wound healing activity. Int J Pharm Pharm Sci, 2, 105-111.
23. Kumari P, Yadav P, Verma PR, et al (2013): A review on wound healing properties of Indian medicinal plants. Ind J Fund Appl Life Sci, 3, 220-232.
24. Kunene P, Mahlambi P (2020): Optimization and application of ultrasonic extraction and Soxhlet extraction followed by solid phase extraction for the determination of triazine pesticides in soil and sediment. J Environ Chem Eng, 8, 103665.
25. Li J, Chen J, Kirsner R (2007): Pathophysiology of acute wound healing. Clin Dermatol, 25, 9-18.
26. Matić S, Stanić S, Bogojević D, et al (2013): Methanol extract from the stem of Cotinus coggygria Scop., and its major bioactive phytochemical constituent myricetin modulate pyrogallol-induced DNA damage and liver injury. Mutat Res Genet Toxicol Environ Mutagen, 755, 81-89.
27. Matić S, Stanić S, Mihailović M, et al (2016): Cotinus coggygria Scop.: An overview of its chemical constituents, pharmacological and toxicological potential. Saudi J Biol Sci, 23, 452-461.
28. Matić S, Stanić S, Solujić S, et al (2011): Biological properties of the Cotinus coggygria methanol extract. Period Biol, 113, 87-92.
29. Moghadam SE, Ebrahimi SN, Salehi P, et al (2017): Wound healing potential of chlorogenic acid and Myricetin-3-O-β-Rhamnoside isolated from Parrotia persica. Molecules, 22, 1501.
30. Mutlu S, Yilmaz E (2019): Yara Yönetiminde Yenilikçi Yaklaşımlar. Gümüşhane Univ Sağlık Bilim Derg, 8, 481-494.
31. Noorbakhsh SI, Bonar EM, Polinski R, et al (2021): Educational Case: Burn Injury—Pathophysiology, Classification, and Treatment. Acad Pathol, 8, 23742895211057239.
32. Reinke JM, Sorg H (2012): Wound repair and regeneration. Eur Surg Res, 49, 35-43.
33. Rodero MP, Khosrotehrani K (2010): Skin wound healing modulation by macrophages. Int J Clin Exp Pathol, 3, 643-653.
34. Rodrigues M, Kosaric N, Bonham CA, et al (2019): Wound healing: a cellular perspective. Physiol Rev, 99, 665-706.
35. Rowan MP, Cancio LC, Elster EA, et al (2015): Burn wound healing and treatment: review and advancements. Crit Care, 19, 1-12.
36. Şahi̇n Y, Zehra G, Alçiğir ME, et al (2023): Effects of functional poly (ethylene terephthalate) nanofibers modified with sericin-capped silver nanoparticles on histopathological changes in parenchymal organs and oxidative stress in a rat burn wound model. Ankara Univ Vet Fak Derg, 70, 131-140.
37. Şekeroğlu V, Atlı Şekeroğlu Z (2011): Micronucleus test for determining genotoxic damage. Turk Bull Hyg Exp Biol, 68, 241-252.
38. Trucillo P, Di Maio E (2021): Classification and Production of Polymeric Foams among the Systems for Wound Treatment. Polymers, 13, 1608.
39. Vural F, Savcı A (2017): Yara bakımında yeni uygulamalar. Turkiye Klinikleri J Surg Nurs Special Topics, 3, 224-232.
40. Wang G, Wang J, Du L, et al (2015): Effect and mechanism of total flavonoids extracted from Cotinus coggygria against glioblastoma cancer in vitro and in vivo. Biomed Res Int, 2015.
41. Wang P-H, Huang B-S, Horng H-C, et al (2018): Wound healing. J Chin Med Assoc, 81, 94-101.
42. Yahaya I, Chemchem M, Aydıner B, et al (2019): Novel fluorescent coumarin-thiophene-derived Schiff bases: Synthesis, effects of substituents, photophysical properties, DFT calculations, and biological activities. J Photochem Photobiol A, 368, 296-306.
43. Yang DJ, Moh SH, Son DH, et al (2016): Gallic acid promotes wound healing in normal and hyperglucidic conditions. Molecules, 21, 899.