Botrytis fabae and Bean common mosaic virus (BCMV) are the most common diseases of Faba bean (Vicia faba L.) in TRNC

Yıl 2017, , 115 - 122, 30.12.2017

Ayşegül Çolak Ateş , Hakan Fidan Nihat Yılmaz Ayda Konuksal

https://doi.org/10.29278/azd.371067

Cited By: 4

Öz

Surveys were conducted on the fields of faba bean culture in Turkish republic of North Cyprus (TRNC) during 2011-2013 indicated that Botrytis fabae and Bean common mosaic virus (BCMV) are the diseases that cause crop loss at economic level. During the field surveys 117 infected plants that have the symptoms of both diseases were collected from 38 different locations. Symptomatologic, morphologic and classic tests indicated that 96 of the faba bean samples had Chocolate spot disease caused by Botrytis fabae and the conidia lengths have been measured between 12.7-22.4 x 9.4-17.8 microns. Faba bean samples with virus symptoms that were collected from 38 locations. All samples were infected by Bean common mosaic potyvirus (BCMV) serologically (DAS-ELISA). The 850 bp section of BCMV isolates coat protein was increased using RT-PCR method. This section was sequenced directly using ClonJet and characterization was determined. According to Blast obtained from sequences 98% and 96 % resemblances with Mexican and Columbian isolates have been found.

Anahtar Kelimeler

Faba bean, Botrytis fabae, Bean common mosaic virüs, PCR

Kaynakça

• Abo-Hegazy, S. R. E., El-Badawy, N.F., Mazen, M. M., Abd El-Menem, H. 2012 Evaluation of Some faba bean genotypes against chocolate spot disease using cDNA fragments of chitinase gene and some traditional methods. Asian J Agric Res, 26:60–72.
• Açıkgöz, S., 1984. Erzincan ve Erzurum yörelerinde Phaseolus vulgaris üzerinde virüslerin tanılanması, yayılışları ve zararları üzerinde araştırmalar. Atatürk Üniversitesi Ziraat Fakültesi (Doktora Tezi). Erzurum, s. 75.
• Anonim 2012. Kuzey Kıbrıs Türk Cumhuriyeti Tarım ve Doğal Kaynaklar Bakanlığı, Bitkisel Üretim Çalıştayı Sonuç Raporu (18-20 Aralık 2012), s.17. web:http://gteb.gov.ct.tr/Portals/1072/BELGELER/Tar
• Anonim, 2013. Food and Agriculture Organization ofThe United Nations, web:http://faostat.fao.org.
• Bhadramurthy, V., Bhat, A. I. 2009. Biological and Molecular Characterization of Bean Common Mosaic Virus Associated with Vanilla in India Indian Journal of Virology, 20(2): 70-77.
• Bhat, A. I., Bhadramurthy. V., Siju, S., Hareesh, P. S. 2006. Detection and identification of Cymbidium mosaic virus infecting vanilla (Vanilla planifolia Andrews) in India based on coat protein gene sequence relationships. J. Plant Biochem. & Biotech. 15: 33-37.
• Biddle, A. J., Cattlin, N. D. 2007. Pest, Diseases and Disorders of Peas and Beans-A Colour Handbook, p. 127., Manson Pub., London.
• Bora, T., Karaca, İ. 1970. Kültür Bitkilerinde Hastalığın ve Zararın Ölçülmesi, E.Ü. Matbaası, İzmir,43s
• Cooper, L. I. and Jones, A.T.1983. Responses of plants to viruses: Proposals for the use of terms. Phytopathology 73, 127-128.
• Davidson, J. A., Pande, S., Bretag, T. W., Lindbeck, K. D., Krishna-Kishore, G. 2007. Biology and Management of Botrytis spp. İn Legume Crops.pp. 303-307. In: Botrytis: Biology, pathology and control, (Elad Y, William-son B, Tudzynski P., Delen N. ed.), Springer, Dordrecht, The Netherlands.
• El-Sayed, A. S., Rania, Z. E., Ismail, A. I. 2011. Fungicidal management of chocolate spot of faba bean and assessment of yield losses due to the disease. Annals of Agricultural Science 56, 27–35
• El-Banoby, F. E., Abd-AllA, M.A., Tolba, I. H., Morsy, A.A., El-Gamal- Nadia, G., Khalil, M. S. A. 2013. Biological Control of Chocolate Spot Disease of Faba Bean Using some Bioagents under Field Conditions. Journal of Applied Sciences Research, 9(6): 4021-4029.
• Fidan, Ü., Yorgancı, Ü. 1990. Investigation on the detection and seed transmission of the virus diseases occurring on the pulse crops in Aegean Region. Seed transmission of virus diseases by grower seeds of artificial infected pulse crops. J. Turkish Phytopath., 19:1-6.
• Gümüş, M., Erkan, S., Yorgancı, Ü., Duman, İ. 2001. Bazı sebzelerin tohumlarında bulunan viral etmenlerin saptanması üzerine araştırmalar. Türkiye IX. Fitopatoloji Kongresi (3-8 Eylül Tekirdağ): 190-197.
• Güzel, Ö., Arlı-Sökmen, M. 2003. Determination of some viruses infecting common bean (Phaseoulus vulgaris L.) and their incidences in seed lots in Samsun Province. J. Turk. Phytopathology. 32: 99-106.
• Harrison, J. G. 1988. The biology of Botrytis spp. on vicia beans and chocolate spot disease, a review. Plant Pathology 37, 168–201.
• Khan, J. A., Lohuis, D., Goldbach, R., Dijkstra, J. 1993. Sequence data to settle the taxonomic position of Bean common mosaic virus and Blackeye cowpea mosaic virus isolates. J.Gen.Virol. 74: 2243-2249.
• Kılıç, H. C., Yardımcı, N. 2014. Burdur İli Bakla Üretim Alanlarında Fasulye Adi Mozayik Virüsü’nün Serolojik ve Moleküler Yöntemlerle Belirlenmesi Türk Tarım ve Doğa Bilimleri Dergisi 1(2): 289–294.
• Koike, S. T. 1998. Severe outbreak of chocolate spot of faba bean, caused by Botrytis fabae, in California. Plant Disease 82 (72), 83– 85.
• Lısa, V., Dellavalle, G., Vaira, A. M., Morales, F. J. 1994. Bean yellow mosaic virus and other viruses in Bean in Western Asia, South-Eastern Europe and Northern China. The Bean Improvement Cooperative Annual Report, 37: 217-218.
• Mink, G. I., Vetten, H. J., Ward, C.W., Berger, P. H., Morales, F. J., Myers, J. M., Silbernagel, M. J., Barnett, O. W. 1994. Taxonomy and classification of legume-infecting potyviruses. A proposal from the Potyviridae Study Group of Plant Virus Sub-committee of ICTV. Arch.Virol. 139: 231-235.
• Morgan, D. T. 1971. Numerical taxonomic studis of the genus Botrytis. Trans. Br. Mycol., Soc., 56(3): 327-335.
• Rahman, M. Z., Honda, Y., Islam, S.Z., Arase, S. 2002. Effect of metabolic inhibitors on red light induced resistance of broad bean (Vicia faba L.) against Botrytis cinerea. J. Phytopathol., 150: 463-468
• Rhaiemi, A., Cherif, M., Kharrat, M., Cherif, M.,Harrabi, M. 2002. New faba bean genotypes resistant to chocolate spot caused by Botrytis fabae. Phytopathol. Mediterr., 41: 99-108.
• Yehia, A. G. M., Mohsen, K. H. E., Magda, M. A. 2004. Influence of Some Plant Extracts and Microbioagents on Some Physiological Traits of Faba Bean Infected with Botrytis fabae. Turk J Bot 28: 519-528.
• Yılmaz, M. A., Özaslan, D. 1987. Fasulye ve börülce tohumlarında afit kökenli mozayik virüsünün ELISA ile saptanması. Çukurova Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümü, 870-873.
• Yu, T. F. 1945. The red-spot disease of broad beans (Vicia faba L.) caused by Botrytis fabae Sardina in China. Phytopathology 35: 945–954.