Doku Kültürü Yöntemiyle Elde Edilen Çilek Bitkilerinin İsmine Doğruluklarının Moleküler Yöntemlerle Belirlenmesi

Öz

Çileğin doku kültürü yöntemleri kullanılarak çoğaltılması son yıllarda önemli ölçüde artmıştır. Özellikle fide üretimi amaçlı in vitro koşullarda üretilen bitkilerin ismine doğruluğu büyük önem arz etmektedir. Moleküler markör yöntemleri kullanılarak laboratuvarda üretilen bitkilerin ismine doğrulukları teşhis edilebilmektedir. Bu araştırmada, ülkemizde ve Yaltır A.Ş.’de yetiştiriciliği yoğun olarak yapılan çilek çeşitleri kullanılmıştır. Doku kültürü laboratuvarında üretimi yapılan bitkiler dış ortama adapte edildikten sonra SSR moleküler markör tekniği kullanılarak ismine doğrulukları belirlenmiştir.

Anahtar Kelimeler

• Çilek
• DNA
• doku kültürü
• SSR

Identification of Genetic Stability of Strawberry Plants Derived by Tissue Culture Techniques

Abstract

Propagation of strawberries using tissue culture techniques has been increased significantly in recent years. The production of truth the name of the strawberry plants by in vitro technique has a great importance for nurseries. Truth the name strawberry plants can be identified using molecular marker methods in the laboratory. In this study, the major cultivars which are grown in our country and Yaltır private company were used as plant sources. The strawberry plants were propagated using tissue culture technique and their truth the name characteristics were detected after transplanted in vivo conditions using SSR molecular markers techniques.

Keywords

• Strawberry
• DNA
• tissue culture
• SSR

Kaynakça

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