THE EFFECTS OF MELATONIN APPLICATIONS ON BEAN SEEDLING DEVELOPMENT UNDER MODERATELY ALKALINE SOIL CONDITIONS

Abstract

In this research, the effect of melatonin (MEL) applications on bean seeds to tolerance of light alkaline soils were investigated. For this purpose, seeds were treated with melatonin (0, 1, 5 or 25 μM) during 24 h. Seeds were sown at 3:1 ratio of peat: perlite mixture (control) and soil that pH 7.94. Seedling length, fresh weight, stem diameter, leaf area, chlorophyll content (SPAD), anthocyanin, proline, MDA, H₂O₂ and electrical conductivity values were measured in bean seedlings. Treatment with 1 μM MEL at the seed stage alleviated the harmful effects of alkaline stress and caused significant improvement in seedling length, fresh weight, chlorophyll content (SPAD), proline, MDA and H₂O₂ content. The best results of stem diameter and leaf area were measured from 25 μM MEL application. The 1 μM MEL application to bean seeds in alkaline soil conditions might be a suggestion for decreasing the stress and damage products.

Keywords

• Bean (Phaseolus vulgaris L.),alkaline stress,alkaline stress,melatonin,proline,MDA,H₂O₂

FASULYE TOHUMLARINA MELATONİN UYGULAMALARININ ORTA ALKALİN TOPRAK KOŞULLARINDA FİDE GELİŞİMİ ÜZERİNE ETKİLERİ

Öz

Bu çalışmada, fasulye tohumlarına melatonin (MEL) uygulamalarının orta alkalin topraklarda toleransı sağlama üzerine etkileri incelenmiştir. Bu amaçla, fasulye tohumları 24 saat boyunca farklı konsantrasyonlardaki MEL (0, 1, 5 veya 25 μM) çözeltisinde bekletilmiştir. Tohumlar 3:1 oranında torf:perlit karışımı (kontrol) ve pH’sı 7.94 olan bahçe toprağına ekilmiştir. Fasulye fidelerinde, fide uzunluğu, taze ağırlık, gövde çapı, yaprak alanı, klorofil içeriği (SPAD), antosiyanin, prolin, lipid peroksidasyon (MDA), H₂O₂ ve elektriki iletkenlik değerleri ölçülmüştür. Tohum aşamasında 1 μM MEL uygulaması alkalin stresinin zararlı etkilerini hafifletmiş ve fide uzunluğu, taze ağırlık, klorofil içeriği (SPAD), prolin, MDA ve H₂O₂ içeriklerinde önemli iyileşme sağlamıştır. Gövde çapı ve yaprak alanı incelendiğinde, en iyi sonucu 25 μM MEL uygulaması vermiştir. Fasulyelerde, alkalin toprak koşullarında tohuma 1 μM MEL uygulanması, stresi ve oluşabilecek hasar ürünlerini azaltmak için önerilebilir.

Anahtar Kelimeler

• Fasulye (Phaseolus vulgaris L.),alkalin stres,melatonin,prolin,MDA,H₂O₂

Kaynakça

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