Phytochemistry and biological activities of the genus Rubus

Deniz Oylumlu; Ali Şen

doi:10.53445/batd.1618598

EN TR

Phytochemistry and biological activities of the genus Rubus

Öz

The genus Rubus, a member of the Rosaceae family, includes important species with both food and medicinal uses. With an emphasis on the anti-inflammatory, anti-cancer, antioxidant, and antibacterial properties of the main chemicals present in Rubus species, this review aims to synthesize the results of recent studies in order to better understand the biological activity and chemical composition of these species and their value as natural bioactive resources. This work also seeks to identify future directions for research that could enhance the clinical applications of Rubus-based bioactive compounds. For this purpose, NCBI, MDPI, ScienceDirect, Google Scholar, MEDLINE and Springer Nature Link databases were searched for recent articles on phytochemical and biological activity studies on Rubus species. The results showed that Rubus species are rich in compounds belonging to different chemical groups (phenolic acids, tannins, flavonoids, anthocyanins, etc.) and extracts and/or secondary metabolites of these species have important biological activities such as antioxidant, antimicrobial, anti-inflammatory, and anticancer effects.

Anahtar Kelimeler

Rubus species, phytochemistry, biological activity

Kaynakça

Akyüz, M. (2022). The determination of antidiabetic, anticholinesterase and antioxidant properties of ethanol and water extracts of blackberry (Rubus fruticosus L.) fruits at different maturity stages. South African Journal of Botany, 151, 1035–1048. https://doi.org/10.1016/j.sajb.2022.11.012
Albert, C., Codină, G. G., Héjja, M., András, C. D., Chetrariu, A., & Dabija, A. (2022). Study of antioxidant activity of garden blackberries (Rubus fruticosus L.) extracts obtained with different extraction solvents. Applied Sciences, 12(8), 4004. https://doi.org/10.3390/app12084004
Álvarez, G. E. G., & Hurtado, N. C. (2024). Secondary metabolites expressed in the fruits of Rubus glaucus Benth grown in Colombia as a defense mechanism against Peronospora sparsa. Scientia Horticulturae, 326. https://doi.org/10.1016/j.scienta.2023.112754
Assafiri, O., Abdallah, H., & El-Dakdouki, M. (2020). Antibacterial effect and phytochemical analysis of the shoot system of Rubus canescens DC. growıng ın lebanon. BAU Journal - Science and Technology, 2(1). https://doi.org/10.54729/2706-784X.1050
Badr, A. M., El-Demerdash, E., Khalifa, A. E., Ghoneim, A. I., Ayoub, N. A., & Abdel-Naim, A. B. (2009). <I>Rubus sanctus</I> protects against carbon tetrachloride-induced toxicity in rat isolated hepatocytes: isolation and characterization of its galloylated flavonoids. Journal of Pharmacy and Pharmacology, 61(11), 1511–1520. https://doi.org/10.1211/jpp/61.11.0011
Bhatt, S. C., Naik, B., Kumar, V., Gupta, A. K., Kumar, S., Preet, M. S., Sharma, N., & Rustagi, S. (2023). Untapped potential of non-conventional rubus species: bioactivity, nutrition, and livelihood opportunities. In Plant Methods (Vol. 19, Issue 1). BioMed Central Ltd. https://doi.org/10.1186/s13007-023-01094-y
Bhuyan, B., & Dutta, A. (n.d.). A review on the phytochemical, pharmacological and traditional profile on the Rubus Genus in north-eastern and western parts of India. In Current Trends in Pharmaceutical Research. www.dibru.ac.in/ctpr
Bolatkyzy, N., Nurmakhanova, A. S., Berganaeva, G. E., & Dyusebaeva, M. A. (2024). Study of the chemical composition of the Rubus vulgaris plant. Chemical Journal of Kazakhstan, 1, 77–88. https://doi.org/10.51580/2024-1.2710-1185.08
Burlando, B., Cornara, L., & Boggia, R. (2023). Nutraceutical potential of high-latitude and high-altitude berries rich in ellagitannins. Current Medicinal Chemistry, 30(19), 2121–2140. https://doi.org/10.2174/0929867329666220224151938
Candela, R. G., Lazzara, G., Piacente, S., Bruno, M., Cavallaro, G., & Badalamenti, N. (2021). Conversion of organic dyes into pigments: Extraction of flavonoids from blackberries (Rubus ulmifolius) and stabilization. Molecules, 26(20). https://doi.org/10.3390/molecules26206278

Čechovičienė, I., Viškelis, J., Viškelis, P., Hallman, E., Kruk, M., & Tarasevičienė, Ž. (2024). Potentially bioactive compounds and sensory compounds in by-products of several cultivars of Blackberry (Rubus fruticosus L.). Horticulturae, 10(8), 862. https://doi.org/10.3390/horticulturae10080862
Choi, G. J., Kang, H., Lee, O. H., & Kwon, J. W. (2023). Effect of immature Rubus occidentalis on postoperative pain in a rat model. Medicina (Lithuania), 59(2). https://doi.org/10.3390/medicina59020264
Das, H., Samanta, A. K., Kumar, S., Roychoudhury, P., Sarma, K., Akter, F., Subudhi, P. K., & Dutta, T. K. (2021). Exploration of antimicrobial, antibiofilm and antiquorum sensing activity of the Himalayan yellow raspberry (Rubus ellipticus) against clinical isolates of Escherichia coli and Staphylococcus aureus. Indian Journal of Animal Research, https://doi.org/10.18805/IJAR.B-4514
De Santis, D., Carbone, K., Garzoli, S., Laghezza Masci, V., & Turchetti, G. (2022). Bioactivity and chemical profile of Rubus idaeus L. leaves steam-distillation extract. Foods, 11(10), 1455. https://doi.org/10.3390/foods11101455
Deliorman Orhan, D., & Hoşbaş Coşkun, S. (2019). The antioxidant activities and total phenol contents of eleven Turkish medicinal plants. Journal of Gazi University Health Sciences Institute, 1(1), 1-9.
George, B. P., & Abrahamse, H. (2019). Increased oxidative stress induced by Rubus bioactive compounds induce apoptotic cell death in human breast cancer cells. Oxidative Medicine and Cellular Longevity, 2019. https://doi.org/10.1155/2019/6797921
Güner, A., Aslan, S., Ekim, T., M Vural, & M Babaç. (2012). Türkiye bitkileri listesi // bizimbitkiler.org.tr - Nezahat Gökyiğit Botanik Bahçesi - 2013. Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını.
Grochowski, D. M., Uysal, S., Zengin, G., & Tomczyk, M. (2019). In vitro antioxidant and enzyme inhibitory properties of Rubus caesius L. International Journal of Environmental Health Research, 29(3), 237–245. https://doi.org/10.1080/09603123.2018.1533532
He, B., Dai, L., Jin, L., Liu, Y., Li, X., Luo, M., Wang, Z., & Kai, G. (2023). Bioactive components, pharmacological effects, and drug development of traditional herbal medicine Rubus chingii Hu (Fu-Pen-Zi). Frontiers in Nutrition, 9. https://doi.org/10.3389/fnut.2022.1052504
Hering, A., Stefanowicz-Hajduk, J., Hałasa, R., Olech, M., Nowak, R., Kosiński, P., & Ochocka, J. R. (2022). Polyphenolic characterization, antioxidant, antihyaluronidase and antimicrobial activity of young leaves and stem extracts from Rubus caesius L. Molecules, 27(19), 6181. https://doi.org/10.3390/molecules27196181
Hl, R. (2019). Antidiabetic and Antioxidant Activity of Rubus apetalus Poir. and Rubus steudneri Schweinf. Leaf Extract on Alloxan Induced Diabetes Mellitus. Article in Journal of Bioanalysis & Biomedicine, 11(2), 149. https://doi.org/10.4172/1948-593X.1000226
Hua, Y. J., Xie, F., Mao, K. jun, Luo, Y. Y., & Ding, Y. J. (2023). Insights into the metabolite profiles of Rubus chingii Hu at different developmental stages of fruit. Journal of Separation Science, 46(16). https://doi.org/10.1002/jssc.202300264
Hummer, K. E. (n.d.). Rubus Pharmacology: Antiquity to the Present. http://visualiseur.bnf.fr/Visua
Huo, Y., Zhao, X., Zhao, J., Kong, X., Li, L., Yuan, T., & Xu, J. (2021). Hypoglycemic effects of Fu-Pen-Zi (Rubus chingii Hu) fruit extracts in streptozotocin-induced type 1 diabetic mice. Journal of Functional Foods, 87. https://doi.org/10.1016/j.jff.2021.104837
Ispiryan, A., Atkociuniene, V., Makstutiene, N., Sarkinas, A., Salaseviciene, A., Urbonaviciene, D., Viskelis, J., Pakeltiene, R., & Raudone, L. (2024). Correlation between Antimicrobial Activity Values and Total Phenolic Content/Antioxidant Activity in Rubus idaeus L. Plants, 13(4), 504. https://doi.org/10.3390/plants13040504
Jaradat, N., Dwikat, M., Amer, J., Hawash, M., Hussein, F., Qneibi, M., Issa, L., Asab, J. A., Hallak, H., Arar, D. N., Masri, H. Z., Obeid, K., Sharabati, M., & Kittaneh, R. (2021a). Anticancer, Free Radicals, and Digestive Enzyme Inhibitory Activities of Rubus sanctus Schreb Root Four Solvent Fractions. Evidence-Based Complementary and Alternative Medicine, 2021. https://doi.org/10.1155/2021/6690646
Jaradat, N., Dwikat, M., Amer, J., Hawash, M., Hussein, F., Qneibi, M., Issa, L., Asab, J. A., Hallak, H., Arar, D. N., Masri, H. Z., Obeid, K., Sharabati, M., & Kittaneh, R. (2021b). Anticancer, Free Radicals, and Digestive Enzyme Inhibitory Activities of Rubus sanctus Schreb Root Four Solvent Fractions. Evidence-Based Complementary and Alternative Medicine, 2021. https://doi.org/10.1155/2021/6690646
Kashchenko, N. I., Olennikov, D. N., & Chirikova, N. K. (2021). Metabolites of Siberian Raspberries: LC-MS Profile, Seasonal Variation, Antioxidant Activity and, Thermal Stability of Rubus matsumuranus Phenolome. Plants, 10(11), 2317. https://doi.org/10.3390/plants10112317
Kim, H., Jung, Y. S., Song, N. E., Yoo, M., Seo, D. H., Kim, H. S., & Nam, T. G. (2024). Ultrasound-assisted extraction of major anthocyanins in Korean black raspberries (Rubus coreanus Miquel) using natural deep eutectic solvents. LWT, 199. https://doi.org/10.1016/j.lwt.2024.116121
Kong, Y., Hu, Y., Li, J., Cai, J., Qiu, Y., & Dong, C. (2022). Anti-inflammatory Effect of a Novel Pectin Polysaccharide From Rubus chingii Hu on Colitis Mice. Frontiers in Nutrition, 9. https://doi.org/10.3389/fnut.2022.868657
Kopjar, M., Raucher, D., Lila, M. A., & Šimunović, J. (2024). Anti-Glioblastoma Potential and Phenolic Profile of Berry Juices. Processes, 12(2). https://doi.org/10.3390/pr12020242
Kumazawa, S., Kurihara, S., Kubota, M., Muto, H., & Hosoya, T. (2024). Anthocyanins and the Antioxidant Capacities of Wild Berries that Grow in Shizuoka, Japan. International Journal of Fruit Science, 24(1), 166–173. https://doi.org/10.1080/15538362.2024.2348716
Li, K., Zeng, M., Li, Q., & Zhou, B. (2019). Identification of polyphenolic composition in the fruits of Rubus chingii Hu and its antioxidant and antiproliferative activity on human bladder cancer T24 cells. Journal of Food Measurement and Characterization, 13(1), 51–60. https://doi.org/10.1007/s11694-018-9918-x
Liu, M., Wang, P., Zhao, B., Gao, X., Meng, N., Li, J., Sun, J., Lu, W., & Sun, B. (2024). Chemical components and health benefits of Rubus suavissimus S. Lee (Chinese sweet tea) and the production method of rubusoside. Trends in Food Science & Technology, 143, 104252. https://doi.org/10.1016/j.tifs.2023.104252
Liu, M., Wu, S., Wu, Y., Zhang, J., Chen, J., Peng, X., Yang, Q., Tan, Z., & Zeng, Z. (2023). Rubus suavissimus S. Lee Extract Alleviates Oxidative Stress and Inflammation in H2O2-Treated Retinal Pigment Epithelial Cells and in High-Fat Diet-Fed Mouse Retinas. Frontiers in Bioscience - Landmark, 28(11). https://doi.org/10.31083/j.fbl2811279
Liu, Z., Mi, Z., Han, N., Zhai, J., Li, S., & Yin, J. (2023). Diterpenoid glucosides with anti-inflammatory activity from Rubi Fructus. Fitoterapia, 164, 105325. https://doi.org/10.1016/j.fitote.2022.105325
Mbaveng, A. T., Damen, F., Simo Mpetga, J. D., Awouafack, M. D., Tane, P., Kuete, V., & Efferth, T. (2019). Cytotoxicity of Crude Extract and Isolated Constituents of the Dichrostachys cinerea Bark towards Multifactorial Drug-Resistant Cancer Cells. Evidence-Based Complementary and Alternative Medicine, 2019. https://doi.org/10.1155/2019/8450158
Mohammad Rahimi, H., Khosravi, M., Hesari, Z., Sharifdini, M., Mirjalali, H., & Zali, M. R. (2020). Anti-Toxoplasma activity and chemical compositions of aquatic extract of Mentha pulegium L. and Rubus idaeus L.: An in vitro study. Food Science and Nutrition, 8(7), 3656–3664. https://doi.org/10.1002/fsn3.1648
Muniyandi, K., George, E., Sathyanarayanan, S., George, B. P., Abrahamse, H., Thamburaj, S., & Thangaraj, P. (2019). Phenolics, tannins, flavonoids and anthocyanins contents influenced antioxidant and anticancer activities of Rubus fruits from Western Ghats, India. Food Science and Human Wellness, 8(1), 73–81. https://doi.org/10.1016/j.fshw.2019.03.005
Paczkowska-Walendowska, M., Gościniak, A., Szymanowska, D., Szwajgier, D., Baranowska-Wójcik, E., Szulc, P., Dreczka, D., Simon, M., & Cielecka-Piontek, J. (2021). Blackberry leaves as new functional food? Screening antioxidant, anti-inflammatory and microbiological activities in correlation with phytochemical analysis. Antioxidants, 10(12). https://doi.org/10.3390/antiox10121945
Pancholi, B., & Rana, A. C. (2020). Traditional Uses, Phytochemistry and Pharmacological Aspects of Rubus niveus thumb Plant – A Review. The Journal of Phytopharmacology, 9(6), 438–444. https://doi.org/10.31254/phyto.2020.9610
Pizzi, A. (2021). Tannins medical / pharmacological and related applications: A critical review. Sustainable Chemistry and Pharmacy, 22. https://doi.org/10.1016/j.scp.2021.100481
Pyeon, S., Kim, O. K., Yoon, H. G., Kim, S., Choi, K. C., Lee, Y. H., Lee, J., Park, J., & Jun, W. (2021). Water extract of Rubus coreanus prevents inflammatory skin diseases in vitro models. Plants, 10(6). https://doi.org/10.3390/plants10061230
Rambaran, T. F., & Ginigini, J. (2020). Essential oil profiles of two Rubus varieties and the antimicrobial activities and lethality of their extracts. ~ 1 ~ American Journal of Essential Oils and Natural Products, 8(3). www.essencejournal.com
Rambaran, T. F., Nembhard, N., Bowen-Forbes, C. S., & Alexander-Lindo, R. L. (2020). Hypoglycemic effect of the fruit extracts of two varieties of Rubus rosifolius. Journal of Food Biochemistry, 44(9). https://doi.org/10.1111/jfbc.13365
Ryan Deweese, Ryan Hunter, Connor Davey, Christina Stacy, Dorota Abramovitch, Diana Ivankovic, & Donna Weinbrenner. (2021). Cytotoxic Effects of Trifolium pratense, Baptisia australis, and Rubus idaeus Extracts on CHO-K1 Cells. GSC Advanced Research and Reviews, 8(1), 128–139. https://doi.org/10.30574/gscarr.2021.8.1.0149
Salah‐Eldin, A. A., Ibrahim, H. H., & Ali, M. R. (2024). Antimicrobial and therapeutic potentials of the blackberry extracts against Escherichia coli infection in male albino rats. Journal of the Science of Food and Agriculture, 104(13), 7776–7787. https://doi.org/10.1002/jsfa.13572
Schmidt-Durán, A., Calvo-Castro, L. A., Alvarado-Ulloa, C., Acosta-Montoya, O., & Rodríguez-Monroy, M. (2023). Cell suspension cultures for the production of antioxidant phenolic compounds: experiments with tropical highland blackberry (Rubus adenotrichos Schltdl. cv. Vino). Plant Cell, Tissue and Organ Culture, 152(3), 669–676. https://doi.org/10.1007/s11240-022-02428-9
Schulz, M., Seraglio, S. K. T., Della Betta, F., Nehring, P., Valese, A. C., Daguer, H., Gonzaga, L. V., Costa, A. C. O., & Fett, R. (2019). Blackberry (Rubus ulmifolius Schott): Chemical composition, phenolic compounds and antioxidant capacity in two edible stages. Food Research International, 122, 627–634. https://doi.org/10.1016/j.foodres.2019.01.034
Shoukat, S., Mahmudıono, T., Al-Shawı, S. G., Abdelbasset, W. K., Yasın, G., Shıchıyakh, R. A., Iswanto, A. H., Kadhım, A. J., Kadhım, M. M., & Al–Rekaby, H. Q. (2022). Determination of the antioxidant and mineral contents of raspberry varieties. Food Science and Technology, 42. https://doi.org/10.1590/fst.118521
Simonovic, M., Kojic, V., Jakimov, D., Glumac, M., & Pejin, B. (2021). Raspberry seeds extract selectively inhibits the growth of human lung cancer cells in vitro. Natural Product Research, 35(13), 2253–2256. https://doi.org/10.1080/14786419.2019.1666391
Subba, B., Gaire, S., & Raj Sharma, K. (2019). Analysıs Of Phyto-Constıtuents, Antıoxıdant, And Alpha Amylase Inhıbıtory Actıvıtıes Of Persea Amerıcana Mıll., Rhododendron Arboretum Sm. Rubus ellipticus Sm. From Arghakhanchı Dıstrıct Nepal. Asian Journal of Pharmaceutical and Clinical Research, 12(1), 301. https://doi.org/10.22159/ajpcr.2019.v12i1.29679
VandenAkker, N. E., Vendrame, S., Tsakiroglou, P., McGilvrey, M., & Klimis-Zacas, D. (2021). Whole Red Raspberry ( Rubus idaeus )-Enriched Diet Is Hepatoprotective in the Obese Zucker Rat, a Model of the Metabolic Syndrome. Journal of Medicinal Food, 24(8), 817–824. https://doi.org/10.1089/jmf.2020.0130
Vega, E. N., Molina, A. K., Pereira, C., Dias, M. I., Heleno, S. A., Rodrigues, P., Fernandes, I. P., Barreiro, M. F., Stojković, D., Soković, M., Carocho, M., Barreira, J. C. M., Ferreira, I. C. F. R., & Barros, L. (2021). Anthocyanins from Rubus fruticosus L. And Morus nigra l. applied as food colorants: A natural alternative. Plants, 10(6). https://doi.org/10.3390/plants10061181
Veličković, I., Živković, J., Stojković, D., Sokovic, M. D., Marin, P. D., & Grujić, S. (2021). Evaluation of Antioxidant, Antimicrobial and Potential Food Preserving Properties of Rubus discolor (Rosaceae) Fruit Extracts. Natural Product Communications, 16(4). https://doi.org/10.1177/1934578X211009692
Wairegi, L. (2024). Rubus fruticosus (blackberry).
Whaley, A. K., Ponkratova, A. O., Orlova, A. A., Serebryakov, E. B., Smirnov, S. N., Proksh, P., Ionov, N. S., Poroikov, V. V., & Luzhanin, V. G. (2021). Phytochemical Analysis of Polyphenol Secondary Metabolites in Cloudberry (Rubus chamaemorus L.) Leaves. Pharmaceutical Chemistry Journal, 55(3), 253–258. https://doi.org/10.1007/s11094-021-02407-y
Yousefbeyk, F., Ghasemi, S., Evazalipour, M., Dabirian, S., Schubert, C., Hekmatnia, S., Habibi, Y., Eghbali Koohi, D., & Böhm, V. (2022). Phytochemical analysis, antioxidant, antibacterial, and cytotoxic activities of leaves and roots of Rubus hyrcanus Juz. European Food Research and Technology, 248(1), 141–152. https://doi.org/10.1007/s00217-021-03866-z
Zhao, F., Zhao, H., Wu, W., Wang, W., & Li, W. (2023). Research on Anthocyanins from Rubus “Shuofeng” as Potential Antiproliferative and Apoptosis-Inducing Agents. Foods, 12(6). https://doi.org/10.3390/foods12061216

Ayrıntılar

Birincil Dil

İngilizce

Konular

Geleneksel, Tamamlayıcı ve Bütünleştirici Tıp (Diğer)

Bölüm

Derleme

Yazarlar

Deniz Oylumlu
0000-0001-7678-7098
Türkiye

Ali Şen ^*
0000-0002-2144-5741
Türkiye

Erken Görünüm Tarihi

8 Mart 2025

Yayımlanma Tarihi

30 Nisan 2025

Gönderilme Tarihi

13 Ocak 2025

Kabul Tarihi

15 Ocak 2025

Yayımlandığı Sayı

Yıl 2025 Cilt: 6 Sayı: 1

DOI

https://doi.org/10.53445/batd.1618598

IZ

https://izlik.org/JA65RG32CP

APA

Oylumlu, D., & Şen, A. (2025). Phytochemistry and biological activities of the genus Rubus. Journal of Integrative and Anatolian Medicine, 6(1), 29-39. https://doi.org/10.53445/batd.1618598

AMA

1.Oylumlu D, Şen A. Phytochemistry and biological activities of the genus Rubus. J. Integr. Anatol. Med. 2025;6(1):29-39. doi:10.53445/batd.1618598

Chicago

Oylumlu, Deniz, ve Ali Şen. 2025. “Phytochemistry and biological activities of the genus Rubus”. Journal of Integrative and Anatolian Medicine 6 (1): 29-39. https://doi.org/10.53445/batd.1618598.

EndNote

Oylumlu D, Şen A (01 Nisan 2025) Phytochemistry and biological activities of the genus Rubus. Journal of Integrative and Anatolian Medicine 6 1 29–39.

IEEE

[1]D. Oylumlu ve A. Şen, “Phytochemistry and biological activities of the genus Rubus”, J. Integr. Anatol. Med., c. 6, sy 1, ss. 29–39, Nis. 2025, doi: 10.53445/batd.1618598.

ISNAD

Oylumlu, Deniz - Şen, Ali. “Phytochemistry and biological activities of the genus Rubus”. Journal of Integrative and Anatolian Medicine 6/1 (01 Nisan 2025): 29-39. https://doi.org/10.53445/batd.1618598.

JAMA

1.Oylumlu D, Şen A. Phytochemistry and biological activities of the genus Rubus. J. Integr. Anatol. Med. 2025;6:29–39.

MLA

Oylumlu, Deniz, ve Ali Şen. “Phytochemistry and biological activities of the genus Rubus”. Journal of Integrative and Anatolian Medicine, c. 6, sy 1, Nisan 2025, ss. 29-39, doi:10.53445/batd.1618598.

Vancouver

1.Deniz Oylumlu, Ali Şen. Phytochemistry and biological activities of the genus Rubus. J. Integr. Anatol. Med. 01 Nisan 2025;6(1):29-3. doi:10.53445/batd.1618598