Elastaz enzimi doğal inhibitörü olarak Defne (Laurus Nobilis L.) ekstraktlarının değerlendirilmesi

Öz

Bu çalışmada son yıllarda sağlık ve kozmetik sektöründe önemli bir yer edinen elastaz enziminin aktivitesi üzerine Defne (Laurus nobilis L.) meyve ve yapraklarından elde edilen ekstraktların (Etanol, Etanol-Su (%70), Aseton, Etilasetat, Hekzan) inhibitör etkileri spektrofotometrik olarak analiz edilmiştir. Hazırlanan tüm ekstraktlar 47,74±0,26 µg/mL ile 29401,36±0,69 µg/mL aralığında değişen IC50 değerleri ile elastaz enzimini inhibe etmiştir. Laurus nobilis L. yapraklarının etanol ile ekstraksiyonundan elde edilen ekstrat 47,74±0,26 µg/mL IC50 değeri ile en kuvvetli elastaz inhibitörü olarak belirlenmiştir. Gerçekleştirilen çalışmada elde edilen veriler ekstratların yüksek antielastaz aktivitesi sayesinde doğal cilt koruması için aktif ve güvenli kozmetik formülasyonlarda ilgi çekici olabileceğini ortaya koymaktadır.

Anahtar Kelimeler

• Elastaz
• Elastin
• Enzim
• İnhibitör
• Defne (Laurus nobilis L.).

Evaluation of Laurel (Laurus Nobilis L.) extracts as natural inhibitors of elastase enzyme

Öz

In this study, the inhibitory effects of extracts (Ethanol, Ethanol-Water (70%), Acetone, Ethylacetate, Hexane) obtained from Laurel (Laurus nobilis L.) fruits and leaves on the activity of elastase enzyme, which has gained an important place in the health and cosmetics sector in recent years, were analyzed spectrophotometrically. All prepared extracts inhibited elastase enzyme with IC50 values ranging from 47,74±0,26 µg/mL to 29401,36±0,69 µg/mL. The extract obtained from ethanol extraction of Laurus nobilis L. leaves was determined to be the most potent elastase inhibitor with an IC50 value of 47,74±0,26 µg/mL. The data obtained in the study reveal that the extracts may be of interest in active and safe cosmetic formulations for natural skin protection thanks to their high antielastase activity.

Anahtar Kelimeler

• Elastase
• Elastin
• Enzyme
• Inhibitor
• Laurel (Laurus nobilis L.).

Kaynakça

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