Cytotoxic Effects of Apiin

Öz

Cancer is the most deadly disease of our age. Existing drugs used for cancer treatment are insufficient in the treatment of cancer due to cumulative mutations. Therefore, there is a need for studies to discover new molecules or to increase drug efficacy in cancer treatment. In terms of the design of new anticancer drugs, the relationship of herbal-derived agents with cancer stands out. It is known that flavonoids taken in our daily diet, together with other components such as various vitamins, play an important role in the prevention of cancer. Flavonoids are thermostable polyphenolic compounds commonly found in nature. Apigenin is an important component of the human diet, such as many fruits and vegetables represented by chamomile, celery and parsley, and it has been shown in various studies to have antitimetastatic and antitumoral effects in many cancers. In our study, Apiin flavonoid belonging to the Apigenin family was tested for the first time on cancer cell lines such as MDAMB231, MCF-7, 293T, 22RV1, U87 at doses of 200, 100, 50 and 25 μg/mL for 24 short-term periods and whether it showed cytotoxic effects.It was observed that Apiin showed selective cytotoxicity in the aforementioned cancer cells. We hope that this study will shed light on other studies on investigating the mechanism of death of Apiin in more detail and as an alternative treatment to cancer treatments.

Anahtar Kelimeler

• Cancer
• phenolic compounds
• apiin
• cytotoxicity

Apiin’in Sitotoksik Etkisi

Öz

Kanser, çağımızın en çok ölümle sonuçlanan hastalığıdır. Kanser tedavisinde kullanılmakta olan ilaçlar, birikerek çoğalan mutasyonlardan dolayı kansere karşı tedavide yetersiz kalmaktadır. Bu nedenle kanser tedavisinde yeni molekülleri keşfetmeye veya ilaç etkinliğini artırmaya yönelik yapılan çalışmalara ihtiyaç duyulmaktadır. Yeni antikanser ilaçların tasarımı bakımınkdan bitkisel türevli ajanların kanserle olan ilişkileri göze çarpmaktadır. Beslenmeyle günlük diyetimizde alınan flavonoidlerin, çeşitli vitaminler gibi diğer bileşenlerle birlikte, kanserin önlenmesinde önemli bir rol oynadığı bilinmektedir. Flavonoidler, doğada yaygın olarak bulunan, ısıya dayanıklı polifenolik bileşiklerdir. Apigenin, papatya, kereviz ve maydanoz ile temsil edilen çok sayıda meyve, sebze gibi insan diyetinin önemli bir bileşenidir ve birçok kanserde antimetastatik ve antitümöral etkilerinin olduğu çeşitli çalışmalarla gösterilmiştir. Çalışmamızda Apigenin ailesine ait olan Apiin flavonoidi ilk kez MDAMB231, MCF-7, 293T, 22RV1, U87 gibi kanser hücre hatları üzerinde 200, 100, 50 ve 25 μg/mL dozlarda 24 saatlik inkübasyon süresinde denenmiş olup, sitotoksik etki gösterip göstermediği test edilmiştir. Apiin’in bahsi geçen kanser hücrelerinde seçici sitotoksisite gösterdiği gözlemlenmiştir. Bu çalışmanın Apiin’in daha ayrıntılı ölüm mekanizmasının araştırılması ve kanser tedavilerine alternatif tedavi olabilmesi konusunda diğer çalışmalara ışık tutacağını umut etmekteyiz.

Anahtar Kelimeler

• Kanser
• fenolik bileşikler
• apiin
• sitotoksisite

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