Psyllobora vigintiduopunctata (L.) (Coleoptera: Coccinellidae)’nın Kabakgillerde külleme hastalığı Erysiphe cichoracearum (DC.) (Helotiales: Erysiphaceae) üzerindeki beslenme performansı

Öz

Psyllobora vigintiduopunctata’nın Erysiphe cichoracearum üzerindeki beslenme kapasitesi, laboratuvar (25±2°C, %70±5 bağıl nem, 12:12 saat fotoperiyot) ve yarı-tarla koşullarında araştırılmıştır. Laboratuvar koşullarında, larva dönemi boyunca tüketilen toplam külleme ile enfekteli alanın yaklaşık 19 cm² olduğu belirlenmiştir. Erginlerin yaşam süreleri boyunca tüketim miktarı incelendiğinde, erkek bireylerin ortalama 195.86±5.48 cm², dişilerin ise 225.14±5.48 cm² enfekteli alan tükettiği saptanmıştır. Yarı-tarla denemeleri, küllemeye karşı P. vigintiduopunctata’nın etkinliğini belirlemek amacıyla enfeksiyonun başlangıç döneminde kabak bitkilerine bitki başına 10, 15 ve 20 ergin birey salınarak yürütülmüştür. Kontrol uygulaması olarak yalnızca saf su kullanılmıştır. Pozitif kontrol olarak, 200g/L azoksistrobin ve 125g/L difenokonazol içeren ticari bir fungisit (Quadris® Maxx, 80ml/100L su) enfekteli bitkilere uygulanmıştır. Kabak bitkilerindeki enfekteli yaprak alanı beş günlük aralıklarla ölçülmüş ve denemeler 20. günde sonlandırılmıştır. Kontrol grubunda bitkilerin 20. günde %100 oranında külleme ile enfekte olduğu belirlenmiştir. Bitki başına 10, 15 ve 20 ergin P. vigintiduopunctata salınan uygulamalarda ortalama hastalık şiddeti 20. günde sırasıyla %16.99–17.07, %12.88–13.42 ve %8.55–8.79 olarak belirlenmiştir. Ortalama etki ise sırasıyla %82.93–83.01, %86.58–87.12 ve %91.21–91.45 olarak hesaplanmıştır. Fungisit uygulanan kabak bitkilerinde ise külleme hastalığı şiddeti 15. günden itibaren artmış, 20. günde yaklaşık %11 hastalık şiddeti ve %89 etkinlik düzeyine ulaşmıştır. Elde edilen bulgular, laboratuvar koşullarında belirlenen beslenme kapasitesi verileri ile uyumlu olup, enfeksiyonun başlangıç döneminde bitki başına 20 ergin P. vigintiduopunctata salımının E. cichoracearum’un baskılanmasında oldukça etkili olduğunu ve kimyasal mücadele stratejilerine güçlü bir alternatif oluşturabileceğini ortaya koymuştur.

Anahtar Kelimeler

• 22 benekli uğur böceği
• salım
• beslenme kapasitesi
• mikofag
• Erysiphe cichoracearum
• Cucurbita pepo

Destekleyen Kurum

Bu çalışma, T.C. Tarım ve Orman Bakanlığı Tarımsal Araştırmalar ve Politikalar Genel Müdürlüğü (TAGEM) tarafından desteklenmiştir.

Proje Numarası

TAGEM/BSAD/E/24/A2/P5/6968

Teşekkür

Külleme hastalığının biyolojik özellikleri konusundaki desteklerinden dolayı Prof. Dr. Fikret Demirci’ye (Ankara Üniversitesi Ziraat Fakültesi, Bitki Koruma Bölümü, Ankara, Türkiye) teşekkür ederiz.

The feeding performance of Psyllobora vigintiduopunctata (L.) (Coleoptera: Coccinellidae) on cucurbit powdery mildew, Erysiphe cichoracearum (DC.) (Helotiales: Erysiphaceae)

Öz

The feeding capacity of Psyllobora vigintiduopunctata on Erysiphe cichoracearum was investigated under laboratory (25 ± 2 °C, 70 ± 5% RH, 12:12 h photoperiod) and semi-field conditions. Under laboratory conditions, the total powdery mildew-infected area consumed approximately 19 cm² over the entire larval period. Over their lifetime, adult males consumed an average of 195.86 ± 5.48 cm² of infected area, whereas females consumed 225.14 ± 5.48 cm². The semi-field experiments were performed to determine the efficacy of P. vigintiduopunctata against powdery mildew by releasing 10, 15, and 20 adults onto squash plants at the initial stage of infection. Only distilled water was used as the control treatment. A commercial fungicide containing 200 g/L azoxystrobin and 125 g/L difenoconazole (Quadris® Maxx), 80 ml/100 L water) was applied to infected plants as the positive control treatment. The infected leaf area on squash plants was measured at five-day intervals, and the trials were terminated on day 20. In the control group, the plants were infected with 100% powdery mildew by day 20. The mean disease severity in plants where 10, 15, and 20 adult P. vigintiduopunctata were released was determined 16.99–17.07%, 12.88–13.42%, and 8.55–8.79%, respectively by 20. day. The mean suppression effects were determined as 82.93–83.01%, 86.58–87.12%, and 91.21-91.45%, respectively. In squash plants that received the fungicide treatment, the severity of powdery mildew increased from day 15 onwards, reaching approximately 11% and 89% effectiveness by day 20. These results were in agreement with laboratory-based feeding capacity data and revealed that releasing 20 adult P. vigintiduopunctata per plant at the initial stage of infection can provide highly effective suppression of E. cichoracearum, representing a strong alternative to chemical control strategies.

Anahtar Kelimeler

• The 22-spotted ladybird
• release
• consumption capacity
• mycophagous
• Erysiphe cichoracearum
• Cucurbita pepo

Destekleyen Kurum

This study was supported by the Ministry of Agriculture and Forestry, General Directorate of Agricultural Research and Policies of Turkiye.

Proje Numarası

TAGEM/BSAD/E/24/A2/P5/6968

Teşekkür

We would like to thank Prof. Dr. Fikret Demirci (Department of Plant Protection, Faculty of Agriculture, Ankara University, Ankara, Türkiye) for his support regarding the biological characteristics of powdery mildew disease.

Kaynakça

1. Abbott W.S., 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18(2), 265–267.
2. Ashraf M., Ishtiaq M., Asif M., Adrees M., Ayub M.N., Mehmood T., Awan M.N., 2010. A study on laboratory rearing of lady bird beetle (Coccinella septempunctata) to observe its fecundity and longevity on natural and artificial diets. International Journal of Biology, 2(1), 165-173.
3. Chi H., Liu H., 1985. Two new methods for the study of insect population ecology. Bulletin of the Institute of Zoology Academia Sinica, 24, 225-240
4. Chi H., 1988. Life table analysis incorporating both sexes and variable development rates among individuals. Environmental Entomology, 17(1), 26-34. https://doi.org/10.1093/ee/17.1.26.
5. Chi H., 2026. CONSUME-MSChart: a computer program for consumption rate analysis based on the age stage, two-sex life table. Web sitesi: https://zenodo.org/records/7484085. (Accessed date: 01 January 2026)
6. De Clercq P., Mason P.G., Babendreier D., 2011. Benefits and risks of exotic biological control agents. BioControl, 56(4), 681–698.
7. Elagamey E., Abdellatef M.A., Haridy M.S., Abd El-aziz E.S.A., 2023. Evaluation of natural products and chemical compounds to improve the control strategy against cucumber powdery mildew. European Journal of Plant Pathology, 165(2), 385-400. https://doi.org/10.1007/s10658-022-02612-9
8. Elderfield J.A.D. Lopez-Ruiz, F.J., van den Bosch F., Cunniffe N.J., 2018. Using epidemiological principles to explain fungicide resistance management tactics: Why do mixtures outperform alternations? Phytopathology, 108(7), 803-817.

9. Huang Y.B., Chi H. 2011. The age-stage, two-sex life table withan offspring sex ratio dependent on female age. Journal of Agriculture and Forestry, 60(4), 337-345.
10. Hanedar A., Tanık A., Girgin E. 2023. Yeşil mutabakat kapsamında pestisit yönetimi ve Türkiye. Çevre İklim ve Sürdürülebilirlik, 24(2), 87-96.
11. Horst R.K. (Ed.), 2013. Westcott's plant disease handbook. Springer Science & Business Media, 1032 p.
12. Kooistra E., 1968. Powdery mildew resistance in cucumber. Euphytica, 17(2), 236-244.
13. Krishnakumar R., Maheswari P., 2004. Management of powdery mildew in mulberry using coccinellid beetles, Illeis cincta (Fabricius) and Illeis bistigmosa (Mulsant). Journal of Entomological Research, 28(3), 241-246.
14. Lebeda A., Křistová E., 1996. Genotypic variation in field resistance of Cucurbita pepo cultivars to powdery mildew (Erysiphe cichoracearum). Genetic Resources and Crop Evolution, 43, 79-84.
15. Lebeda A., McGrath M.T., Sedlakova B., 2010. Fungicide resistance in cucurbit powdery mildew fungi. Fungicides, 11(2), 221-246.
16. Liu C.L., 1951. Studies on the feeding capacity and life history of a mycophagous coccinellid, Halyzia hauseri Mader, in Kunming. Annales Entomologici Sinici, 1, 1-15.
17. MAF, 2025a. Republic of Türkiye Ministry of Agriculture and Forestry. Kabakgillerde külleme hastalığı [Erysiphe cichoracearum D.C., Podosphaera xanthii (Castagne) U.Braun and Shishkoff] standart ilaç deneme metodu sf. 63-65. https://www.tarimorman.gov.tr/TAGEM/Belgeler/Sitandard/Sebze%20Hastal%C4%B1klar%C4%B1%20Standart%20%C4%B0la%C3%A7%20Deneme%20Metotlar%C4%B1.pdf. (Accessed date: 09 December 2025)
18. MAF, 2025b. Republic of Türkiye Ministry of Agriculture and Forestry. Bitki koruma ürünleri veri tabanı. https://bku.tarimorman.gov.tr/BKURuhsat/Details/5331. (Accessed date: 09 December 2025)
19. McGrath M.T., 2001. Fungicide resistance in cucurbit powdery mildew: experiences and challenges. Plant Disease, 85(3), 236-245.
20. Newark M.J., Li P., Yang X.P., Paret M.L., Dufault N.S., 2020. Comparing Stagonosporopsis spp. fungicide resistance profiles in Florida and East China cucurbit production systems. Plant Disease, 104(1), 129-136. https://doi.org/10.1094/PDIS-02-19-0370-RE
21. Pérez‐Garcia A., Romero D., Fernández‐Ortuño D., Lopez‐Ruiz F., De Vicente A., A Tores J., 2009. The powdery mildew fungus Podosphaera fusca (synonym Podosphaera xanthii), a constant threat to cucurbits. Molecular Plant Pathology, 10(2), 153-160.
22. Ratti E., 1996. Coleoptera associated with Oidium evonymi-japonici (Arc.) Sacc. (fungi imperfecti) in the urban gardens of Venice. Bollettino del Museo Civico di Storia Naturale di Venezia, 45, 47-51.
23. Sarhan E.A., Abd-Elsyed M. H., Ebrahiem A. M., 2020. Biological control of cucumber powdery mildew (Podosphaera xanthii) (Castagne) under greenhouse conditions. Egyptian Journal of Biological Pest Control, 30(65), 1-7. https://doi.org/10.1186/s41938-020-00267-4
24. Sutherland, A.M., 2005. Evaluation of Psyllobora vigintimaculata (Say) (Coleoptera: Coccinellidae) for biological control of powdery mildew fungi (Erysiphales). Master thesis. M.S. Horticulture and Agronomy, 45.
25. Sutherland A.M., Parrella M. P., 2006. Quantification of powdery mildew consumption by a native Coccinellid: Implications for biological control integrated control in protected crops, Mediterranean Climate IOBC/wprs Bulletin, 29(4), 281-286.
26. Sutherland A.M., 2009. The Feasibility of Using Psyllobora vigintimaculata (Coleoptera: Coccinellidae), a Mycophagous Ladybird Beetle, for Management of Powdery Mildew Fungi (Erysiphales). PhD Thesis, 99 p., California.
27. Soylu S., Yigit A., 2002. Feeding of mycophagous ladybird, Psyllobora bisoctonotata (Muls.) on powdery mildew infested plants. International Organization for Biological Control Western Palearctic Regional Section Bulletin, 25(10), 183-186.
28. Syngenta, 2025. Syngenta, Quadris Maxx Label information. https://www.syngenta.com.tr/sites/g/files/kgtney1481/files/media/document/2025/10/20/Quadris_Maxx_etiket%201.pdf. (Accessed date: 09 December 2025)
29. Yalçın, F., Ülgentürk, S., 2023. Mikofag Coccinellidae (Coleoptera) türleri ve biyolojik mücadeledeki potansiyelleri. Bursa Uludağ Üniversitesi Ziraat Fakültesi Dergisi, 37(2), 519-535. https://doi.org/10.20479/bursauludagziraat.1275933
30. Yalçın, F., Ülgentürk, S., Özgökçe, M.S., 2026. Interactions between Psyllobora vigintiduopunctata (L.) (Coleoptera: Coccinellidae) and cucurbit powdery mildew. Plant Protection Bulletin, 66(1), 1-9. https://doi.org/10.16955/bitkorb.1758992
31. Younes G.H., Ahmad M., Ali N., 2015. Morphological, biological and ecological studies of the mycophagous ladybird Psyllobora vigintiduopunctata L. (Coleoptera: Coccinellidae) on powdery mildew fungi in the coastal region of Suriye. Jordan Journal of Agricultural Sciences, 11(2), 483-494. DOI: 10.12816/0030440.
32. Yücesoy M., Tek M., Çalış Ö., 2020. Determination and identification of powdery mildews on domestic, wild and commercial cucurbits. Mediterranean Agricultural Sciences, 33(2), 207-214. https://doi.org/10.29136/mediterranean.711786.