Determination of imidacloprid and indoxacarb residues in apple and it’s processed products

Yıl 2019, , 23 - 32, 29.06.2019

Osman Tiryaki , Ezgi Özel

https://doi.org/10.16955/bitkorb.465828

Cited By: 4

Öz

Pesticide residues are important in raw agricultural products and their processed products. In this study, imidacloprid and indoxacarb pesticides were applied to "Golden Delicious" apples. Residue analyses were performed by using QuEChERS method and LC-MS/MS. In apples, imidacloprid and indoxacarb residues (mean of sampled 3 days after the application and 14 days after the application), were 170.60 and 490.77 µg/kg, respectively. Imidacloprid residues in the peel, pulp, pre-juicing, full processed-juice and bagasse were 791.14, 104.17, 44.60, 47.41 and 262.57 µg/kg, respectively, as the mean of both harvest periods. For indoxacarb, residues were 2195.07 µg/kg in the peel, 197.84 µg/kg in the pulp, 82.26 µg/kg in the pre-juicing and 597.17 µg/kg in the bagasse. The processing factors (Pf) of each process were calculated as a reduction or concentration factor. Imidacloprid residues in the peel and baggase were 4.67 and 1.55 times (mean of two harvesting time) higher than apple, respectively.The residues of pulp, pre-juicing, full processed-juice reduced by 39.75%, 73.54% and 70.62%, respectively. Indoxacarb residues in the peel and baggase were increased 4.46 and 1.21 times, respectively. The mean reduction rates were 59.84% in pulp and 83.35% in pre-juicing. The difference between the decrease in residual reduction rates and the rate of residual degradation between the harvest process in the full process fruit juice was significant at 1% level. The residues of processes, calculated based on Pf, were below MRL (500 µg/kg) for both insecticides. Residues in peel and baggase were increased regardless of harvest period and pesticide.

Anahtar Kelimeler

Pesticide residue, QuEChERS, imidacloprid, indoxacarb, apple, processing factor

Elma ve işlenmiş ürünlerinde imidacloprid ve indoxacarb kalıntılarının belirlenmesi

Öz

Tarım
ürünlerinde ve bunların işlenmiş ürünlerinde pestisit kalıntıları önemlidir. Bu
çalışmada "Golden Delicious" çeşidi elmalar imidacloprid ve
indoxacarb etkili maddeli bitki koruma ürünleri ile ilaçlanmıştır. QuEChERS
metodu ve LC-MS/MS ile kalıntı analizleri yapılmıştır. Elmalarda imidacloprid
ve indoxacarb kalıntıları (pestisit uygulamasından 3 gün sonra ve 14 gün sonra
hasat edilen örneklerin ortalaması), sırasıyla, 170.60 ve 490.77 µg/kg olarak
bulunmuştur. Imidacloprid kalıntıları iki hasat döneminin ortalaması olarak
elma kabuklarında 791.14 µg/kg, meyve etinde 104.17 µg/kg, ilk sıkım meyve
suyunda 44.60 µg/kg, tam proses meyve suyunda 47.41 µg/kg ve posada 262.57
µg/kg olarak bulunmuştur. Indoxacarb için bu kalıntılar kabukta 2195.07 µg/kg,
meyve etinde 197.84 µg/kg, ilk sıkım meyve suyunda 82.26 µg/kg ve posada 597.17
olarak bulunmuştur. İşleme faktörleri (İf) azalma ya da konsantrasyon faktörü
olarak hesaplanmıştır. Kabuk ve posada imidacloprid kalıntıları elmaya göre
sırasıyla, ortalama 4.67 ve 1.55 katı daha fazla bulunmuştur. Kabuksuz meyve
eti, ilk sıkım meyve suyu ve tam proses meyve suyu kalıntıları, elmaya göre
ortalama olarak, sırasıyla, %39.75, %73.54 ve %70.62 oranında azalmıştır.
Indoxacarb için ise kabuk ve posada kalıntı, sırasıyla, ortalama olarak 4.46 ve
1.21 kat artmıştır. Ortalama azalma oranları meyve etinde %59.84, ilk sıkım
meyve suyunda %83.35 olarak bulunmuştur. Kabuksuz meyve, ilk sıkım ve tam
proses elma suyunda, kalıntı azalma oranları arasındaki fark ile tam proses
meyve suyunda hasat dönemleri arasındaki azalma oranları arasındaki fark %1
düzeyinde önemlidir. İşlenmiş elmalarda İf’ye bağlı olarak yapılan
hesaplamalarda bulunan kalıntı değerleri her iki insektisit için MRL’nin (500
µg/kg) altında bulunmuştur. Hasat dönemine ve pestisite bağlı olmaksızın
kabukta ve elma posasında kalıntılar artmıştır.

Anahtar Kelimeler

Pestisit kalıntısı, QuEChERS, imidacloprid, indoxacarb, elma, işleme faktörü

Kaynakça

• Anastassiades M., Lehotay S.J., Stajnbaher D., Schenck F.J., 2003. Fast and easy multiresidue method employing acetonitrile extraction/partitioning and dispersive solid-phase extraction for the determination of pesticide residues in produce. J.of AOAC Int. 86, 412–431.
• Anastassiades M., Scherbaum E., Tasdelen B., Stajnbaher D., 2007. Wiley-VCH, Weinheim. In: Ohkawa H., Miyagawa H., Lee P.W. (Eds.). Crop Protection, Public Health, Environmental Safety. Germany. 439 P.
• Bajwa U., Sandhu K.S., 2014. Effect of handling and processing on pesticide residues in food- A Review. Journal Food Science Technology, 51 (2), 201–220.
• Bonnechère A., Hanot V., Jolie R., Hendrickx M., Bragard C., Bedoret T., Loco J. V., 2012a. Effect of household and industiral processing on levels of five pesticide residues and two degradation products in spinach. Food Control, 25, 397-406.
• Bonnechère A., Hanot V., Bragard C., Bedoret T., Loco J.V., 2012b. Effect of household and industrial processing on the levels of pesticide residues and degradation products in melons. Food Add.& Contam., 29 (7), 1058-1066.
• Buckingham S., Lapied B., Corronc H., Sattelle F., 1997. Imidacloprid actions on insect neuronal acetylcholine receptors. The Journal of Experimental Biology, 200, 2685-2692.
• Burchat C., S., Ripley B.D., Leishman P.D., Ritcey G.M., Kakuda Y., Stephenson G.R., 1998. The distribution of nine pesticides between the juice and pulp of carrots and tomatoes after home processing. Food Additives & Contaminants, 15 (1), 61-71.
• Dong F., 2012 The pesticide residue changes during food processing and storage https://selamat.net/upload_mm/9/0/9f1f3d226-b38f-49fb-9d1e-f7eccfe34797_ ma6.pdf. (Erişim tarihi: 03.06.2018).
• EC 2002. Commission establishing community methods of sampling for the official control of pesticide residues in and on products of plant and animal origin and repealing. Directive 2002/63/EC of 11 July 2002.
• EFSA 2014. Scientific Report of EFSA. The 2012 European Union Report on Pesticide Residues in Food. EFSA Journal, EFSA Journal 2016, 14(10), 4611.
• Ghani S., B., A., Hanafi A., Nasr N., 2010. Non-Toxic washing solutions for decreasing myclobutanil, fenhexamid and boscalid residues in sweet pepper and cherry tomatoes. Australian Journal of Basic and Applied Sciences, 4 (8), 3360-3365.
• Kaushik G., Satya S., Naik S.N., 2009. Food processing a tool the pesticide residue dissipation – A Review. Food Research International, 42, 26–40.
• Kharandi, N., Babri, M., Azad, J., 2013. A novel method for determination of patulin in apple juices by GC-MS. Food Chemistry, 141 (3), 1619-1623.
• Lapied B., Grolleau F., Sattelle D.B., 2001. Indoxacarb, an oxadiazine ınsecticide, blocks insect neuronal sodium channels. Br J Pharmacol, 132 (2), 587-95.
• Lehotay S., J., Maśtovská K., Lightfield A., R., 2005. Use of buffering and other means to ımprove results of problematic pesticides in a fast and easy method for residue analysis of fruits and vegetables. Journal of AOAC International, 88 (2), 615-629.
• Martin L., Mezcua M., Ferrer C., Gil Garcia M. D., Malato O. and Fernandez-Alba A. R., 2013. Prediction of the processing factor for pesticides in apple juice by principal component analysis and multiple linear regression. Food Additives and Contaminants, 30 (3), 466.
• OECD 2008. Magnitude of the pesticide residues in processed commodities. OECD Guideline for the Testing of Chemicals. http://www.oecd-ilibrary.org/environment/test-no-508-magnitude-of-the-pesticide-residues-in-processed-commodities_9789264067622-en (Erişim tarihi: 25.07.2018).
• Özel E., 2018. Elma ve işlenmiş ürünlerinde imidacloprid ve indoxacarb kalıntılarının araştırılması. Çanakkale Onsekiz Mart Üniversitesi Fen Bilimleri Enstitüsü, Basılmamış Yüksek Lisans Tezi, 58 s., Çanakkale.
• PPDB 2018. PPDB: Pesticide Properties Database. Retrieved March 18, 2018. http://Sitem.Herts.Ac.Uk/Aeru/Ppdb/En/Reports/154.htm. (Erişim tarihi: 03.06.2018).
• RASFF 2018. EU Rapid Alert System For Food And Feed. https://webgate.ec.europa. eu/rasff window/portal/?event=SearchForm&cleanSearch=1 (Erişim tarihi: 03.06.2018.
• Romeh A.A., Mekky T.M., Ramadan RA., Hendawi MY., 2009. Dissipation of profenofos, imidacloprid and penconazole in tomato fruits and products. Bull Env Cont Toxic, 83, 812–817.SANTE 2017. Guidance document on analytical quality control and method validation procedures for pesticides residues analysis in food and feed. sante/11813/2017. https://ec.europa.eu/food/sites/food/files/plant/docs/pesticides_mrl_guidelines_wrkdoc_2017-11813.pdf (Erişim Tarihi 26.03.2018).
• Saravi S., Shokrzadeh M., 2016. Effects of washing, peeling, storage and fermentation on residue contents of carbaryl and mancozeb in cucumbers grown in greenhouses. Toxicology and Industrial Health, 32 (6), 1135–1142.
• Scholz R., Herrmann M., Michalski B., 2017. Compilation of processing factors and evaluation of quality controlled data of food processing studies. J Consum Prot Food Saf., 12, 3–14.
• Tiryaki, O., 2016. Validation of quechers method for the determination of some pesticide residues in two apple varieties. J Environmental Sci and Health B, 51(10), 722-729.Tiryaki O., 2017. Analiz metodunun validasyonu ve metot performans kriterleri. Pestisit Kalıntı Analizlerinde Kalite Kontrol (QC) Ve Kalite Güvencesi (QA). Nobel Yayın No: 1697, Fen Bilimleri: 129, 2. Basım: 121-156.
• Tiryaki, O., Baysoyu, D., Seçer, E., Aydın, G., 2008. Testing the stability of pesticides during sample processing for the chlorpyrifos and malathion residue analysis in cucumber, including matrix effects. Bull Environ Contam Toxicol , 80 (1), 38-43