Determination of the insecticide resistance mechanisms by biochemical assays in <em>Myzus persicae</em> (Sulz.) (Hemiptera:Aphididae) populations collected from Icel-Turkey

Cilt: 48 Sayı: 3 10 Kasım 2009
  • A. Velioğlu
  • G. Moores
  • A. Devonshıre
  • S. Toros
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Determination of the insecticide resistance mechanisms by biochemical assays in <em>Myzus persicae</em> (Sulz.) (Hemiptera:Aphididae) populations collected from Icel-Turkey

Öz

The carboxylesterase correlated with insecticide resistance in different Myzus persicae populations collected from Icel were analysed by using microplate reader and electrophoresis. Total carboxylesterase activities and carboxylesterase E4 band patterns of the populations were determined and the values were found as extremely high levels at all of Icel populations. Results of the detailed analyses from microplate assay, immunoassay and electrophoresis showed that Icel populations have very high levels and FE4 type of carboxylesterase.
Icel populations were also examined the case of insecticide resistance resulted from insensitivity of acetylcholinesterase (AChE). It was found that three Icel populations have insensitive AChE to pirimicarb. Icel populations showed “multiple resistance mechanismâ€?, because of high carboxylesterase activities and also insensitive AChE.

Anahtar Kelimeler

Kaynakça

  1. Anonymous, 2008. http://www.rothamsted.ac.uk/insect-survey/STMyzus_persicae.php.
  2. Anstead, J. A., Williamson, M. S., Eleftherianos, I. and Denholm, I. 2004. High-throughput detection of knockdown resistance in Myzus persicae using allelic discriminating quantitative PCR. Insect Biochemistry and Molecular Biology, 34, 871–877.
  3. Bizzaro, D., Mazzoni, E., Barbolini, E., Giannini, S., Cassanelli, S., Pavesi, F., Cravedi, P. and Manicardi, G. C. 2005. Relationship among expression, amplification, and methylation of FE4 esterase genes in Italian populations of Myzus persicae (Sulzer) (Homoptera: Aphididae). Pesticide Biochemistry and Physiology, 81, 5158.
  4. Criniti, A., Mazzoni, E., Cassanelli, S., Cravedi, P., Tondelli, A., Bizzaro, D. and Manicardi, G. C. 2008. Biochemical and molecular diagnosis of insecticide resistance conferred by esterase, MACE, kdr and super- kdr based mechanisms in Italian strains of the peach potato aphid, Myzus persicae (Sulzer). Pesticide Biochemistry and Physiology, 90, 168-174.
  5. Devine, G. and Devonshire, A. L. 1994. Immunoassay protocol for characterising insecticide-resistant Myzus persicae. 5 p., (basılmamış laboratuvar protokolu).
  6. Devonshire, A. L. 1975. Studies of the carboxylesterases of Myzus persicae resistant and susceptible to organophosphorus insecticides. Proceedings 8th British Insecticide and Fungicide Conference, 67-73.
  7. Devonshire, A. L. 1977. The properties of a carboxylesterase from the peach-potato aphid, Myzus persicae (Sulz.), and its role in conferring insecticide resistance. Biochem. J., 167, 675-683.
  8. Devonshire, A. L. and Moores, G. D. 1982. A carboxylesterase with broad substrate specificity causes organophosphorus, carbamate and pyrethroid resistance in peach-potato aphids (Myzus persicae). Pesticide Biochemistry and Physiology, 18, 235-246.

Ayrıntılar

Birincil Dil

İngilizce

Konular

-

Bölüm

-

Yazarlar

A. Velioğlu Bu kişi benim

A. Devonshıre Bu kişi benim

Yayımlanma Tarihi

10 Kasım 2009

Gönderilme Tarihi

10 Kasım 2009

Kabul Tarihi

-

Yayımlandığı Sayı

Yıl 2008 Cilt: 48 Sayı: 3

Kaynak Göster

APA
Velioğlu, A., Moores, G., Devonshıre, A., & Toros, S. (2009). Determination of the insecticide resistance mechanisms by biochemical assays in <em>Myzus persicae</em> (Sulz.) (Hemiptera:Aphididae) populations collected from Icel-Turkey. Plant Protection Bulletin, 48(3), 15-31. https://izlik.org/JA69XT62ZG
AMA
1.Velioğlu A, Moores G, Devonshıre A, Toros S. Determination of the insecticide resistance mechanisms by biochemical assays in <em>Myzus persicae</em> (Sulz.) (Hemiptera:Aphididae) populations collected from Icel-Turkey. Plant Protection Bulletin. 2009;48(3):15-31. https://izlik.org/JA69XT62ZG
Chicago
Velioğlu, A., G. Moores, A. Devonshıre, ve S. Toros. 2009. “Determination of the insecticide resistance mechanisms by biochemical assays in <em>Myzus persicae</em> (Sulz.) (Hemiptera:Aphididae) populations collected from Icel-Turkey”. Plant Protection Bulletin 48 (3): 15-31. https://izlik.org/JA69XT62ZG.
EndNote
Velioğlu A, Moores G, Devonshıre A, Toros S (01 Aralık 2009) Determination of the insecticide resistance mechanisms by biochemical assays in <em>Myzus persicae</em> (Sulz.) (Hemiptera:Aphididae) populations collected from Icel-Turkey. Plant Protection Bulletin 48 3 15–31.
IEEE
[1]A. Velioğlu, G. Moores, A. Devonshıre, ve S. Toros, “Determination of the insecticide resistance mechanisms by biochemical assays in <em>Myzus persicae</em> (Sulz.) (Hemiptera:Aphididae) populations collected from Icel-Turkey”, Plant Protection Bulletin, c. 48, sy 3, ss. 15–31, Ara. 2009, [çevrimiçi]. Erişim adresi: https://izlik.org/JA69XT62ZG
ISNAD
Velioğlu, A. - Moores, G. - Devonshıre, A. - Toros, S. “Determination of the insecticide resistance mechanisms by biochemical assays in <em>Myzus persicae</em> (Sulz.) (Hemiptera:Aphididae) populations collected from Icel-Turkey”. Plant Protection Bulletin 48/3 (01 Aralık 2009): 15-31. https://izlik.org/JA69XT62ZG.
JAMA
1.Velioğlu A, Moores G, Devonshıre A, Toros S. Determination of the insecticide resistance mechanisms by biochemical assays in <em>Myzus persicae</em> (Sulz.) (Hemiptera:Aphididae) populations collected from Icel-Turkey. Plant Protection Bulletin. 2009;48:15–31.
MLA
Velioğlu, A., vd. “Determination of the insecticide resistance mechanisms by biochemical assays in <em>Myzus persicae</em> (Sulz.) (Hemiptera:Aphididae) populations collected from Icel-Turkey”. Plant Protection Bulletin, c. 48, sy 3, Aralık 2009, ss. 15-31, https://izlik.org/JA69XT62ZG.
Vancouver
1.A. Velioğlu, G. Moores, A. Devonshıre, S. Toros. Determination of the insecticide resistance mechanisms by biochemical assays in <em>Myzus persicae</em> (Sulz.) (Hemiptera:Aphididae) populations collected from Icel-Turkey. Plant Protection Bulletin [Internet]. 01 Aralık 2009;48(3):15-31. Erişim adresi: https://izlik.org/JA69XT62ZG

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