DERİ KANSERİ HASTALARININ SERUM OKSİDATİF STRES PARAMETRELERİNİN DEĞERLENDİRİLMESİ Evaluation of Serum Oxidative Stress Parameters of Skin Cancer Patients

Yıl 2018, Cilt: 8 Sayı: 4, 134 - 138, 15.12.2018

İbrahim Halil Yavuz Göknur Özaydın Yavuz Serap Güneş Bilgili Halit Demir Canan Demir

https://doi.org/10.16919/bozoktip.452100

Cited By: 1

Öz

Giriş ve amaç: Deri kanserleri en sık görülen kanserlerden biridir. Bu kanserler non melanom

ve melanom olarak genellikle iki grup altında incelenir ve çoğu nonmelanomadır. Deri kanserlerinin

insidansı ve mortalite oranları tüm dünyada giderek artmaktadır. Bu çalışmada hücre içi

antioksidanlar olan superoksit dismutaz (SOD), katalaz (CAT), redükte glutatyon (GSH), glutatyon

redüktaz (GR), glutatyon peroksidaz (GPx) gibi moleküller ile oksidatif stres belirteci olan malondialdehit

(MDA) değerlerini deri kanseri hastalarında incelemek istedik.

Materyal ve metod: Prospektif çalışma modifiye edilen Dünya Helsinki Deklarasyonu'na göre

uygulandı. Bu çalışma üniversite hastanesinin dermatoloji departmanında yapıldı. Çalışmaya 30

deri kanseri hastası ve 30 sağlıklı gönüllü alındı. Çalışmaya katılanların serumlarında hücre içi

antioksidantlar ve malondialdehit seviyeleri değerlendirildi

Bulgular: Çalışma 33 erkek (%55), 27 kadın (%45) olmak üzere toplam 60 kişi ile gerçekleştirilmiştir.

Çalışmaya katılan kişilerin hasta ve kontrol grubuna göre yaş ve cinsiyet değişkenleri

karşılaştırılmış aralarında anlamlı farklılık saptanmamıştır (p>0.05). GPx, GR, GSH, SOD, MDA,

CAT değerlerinin, hasta ve kontrol grup ortalamaları arasındaki fark istatistik olarak önemli bulunmuştur

(p<0.05).

Sonuç: Bu çalışma deri kanseri hastalarında antioksidan serum düzeylerinin azaldığını ve oksidatif

stres belirteci olan MDA’nın yüksek olduğunu göstermiştir.

Anahtar Sözcu¨kler: Deri kanseri; Superoksit dismutaz; Katalaz

ABSTRACT

Introduction and objectives: Skin cancers are one of the most common cancers. These

cancers are usually examined under two groups as non-melanomas and melanomas, and most

nonmelanomas are . The incidence and mortality rates of skin cancers are increasing all over the

world. In this study, we want to investigated the intracellular antioxidants such as superoxide

dismutase (SOD), catalase (CAT), reductase glutathione (GSH), glutathione reductase (GR),

glutathione peroxidase (GPx) and malondialdehyde (MDA) values, which are oxidative stress

markers, in skin cancer patients

Material and method: The prospective study was carried out according to the modified World

Helsinki Declaration. This study was done in the dermatology department of the university

hospital. Thirty skin cancer patients and 30 healthy volunteers were included in the study.

Intracellular antioxidants and levels of malondialdehyde were evaluated of participants in the

study

Results: The study consisted of 33 men (55%) and 27 women (45%). There was no significant

difference between the age and gender variables of the study participants according to the

patient and control group (p>0.05). The difference between GPx, GR, GSH, SOD, MDA, CAT

values, patient and control group averages were statistically significant (p<0.05).

Conclusion: This study has shown that antioxidant serum levels are decreased in skin cancer

patients and MDA, an oxidative stress marker, is high.

Keywords: Skin cancer; Superoxide dismutase; Catalase

Bu çalışma Yüzüncüyıl Üniversitesi Bilimsel Araştırma Başkanlığı tarafından, 2015-TF-B321 numaralı

proje ile desteklenmiştir.

Anahtar Kelimeler

Deri kanseri, superoksit dismutaz, katalaz

Kaynakça

• 1-Linares MA, Zakaria A, Nizran P. Skin Cancer. Prim Care. 2015;42(4):645-59.
• 2-Marks R. An overview of skin cancers. Incidence and causation. Cancer. 1995;15;75(2 Suppl):607-12.
• 3-Basel H, Kavak S, Demir H, Meral I, Ekim H, Bektas H. Effect of levosimendan injection on oxidative stress of rat myocardium. Toxicol Ind Health. 2013;29(5):435-40.
• 4-Sander CS, Chang H, Hamm F, Elsner P, Thiele JJ. Role of oxidative stress and the antioxidant network in cutaneous carcinogenesis. Int J Dermatol. 2004;43(5):326-35.
• 5-Kryston TB, Georgiev AB, Pissis P, Georgakilas AG. Role of oxidative stres and DNA damage in human carcinogenesis. Mutat Res. 2011;711(1-2):193-201.
• 6-Waris G, Ahsan H. Reactive oxygen species: role in the development of cancer and various chronic conditions. J Carcinog. 2006;5:14.
• 7-Sorg O. Oxidative stress: a theoretical model or a biological reality? C R Biol.2004 ;327(7):649-62.
• 8- Williams, J.A., Wiener, G., Anderson, P.H., McMurray, C.H.,. Variation in the activities of glutathione peroxidase and superoxide dismutase and in the concentration of copper in the blood in various breed crosses of sheep. Research Veterinary Science. 1983; 34: 253–256
• 9-Aebi H. Catalase In: Packer L, editor.Methods in enzymology. Orlando: Academic Press; 1984. p. 105. 121-6.
• 10-Beutler, E., Duron, O., Kelly, B.M.,. Improved method for the determination of blood glutathione. J Lab Clin Med 1963;61: 882-90.
• 11-Paglia D.E., Valentine W.N.,.Studies on the quantitative and qualitative charaterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967;70 (19) : 158-169.12- Gutteridge, J.M.,.Lipit peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem. 1995;41(12): 1819–1828.
• 13-Oberley L.W. Representetive of Polypeptid Structure of Bovine CuZnSOD. Superoxide Dismutase, 1982;1:28
• 14-Younus H. Therapeutic potentials of superoxide dismutase. International Journal of Health Sciences. 2018;12(3):88-93.
• 15-Darwish, Hossam & Toson, Elshahat & Hatem, A & T El Zanaty, Ghada & A Abdel, Camelia. oxidative stress-Antioxidant status in Egyptian Lymphoma patients.. Nature and Science of Sleep. 2012;10:31-37
• 16-Sander çalışma Sander CS, Hamm F, Elsner P, Thiele JJ. Oxidative stress in malignant melanoma and non-melanoma skin cancer. Br J Dermatol. 2003 ;148(5):913-22.
• 17-Abou-Seif MA, Rabia A, Nasr M. Antioxidant status, erythrocyte membrane lipid peroxidation and osmotic fragility in malignant lymphoma patients. Clin Chem Lab Med. 2000;38(8):737-42.
• 18-Glorieux C, Calderon PB. Catalase, a remarkable enzyme: targeting the oldest antioxidant enzyme to find a new cancer treatment approach. Biol Chem. 2017;398(10):1095-1108.
• 19-Jayashree G, Kurup Muraleedhara G, Sudarslal S, Jacob VB. Anti-oxidantactivity of Centella asiatica on lymphoma-bearing mice. Fitoterapia. 2003 ;74(5):431-4.
• 20-Shen Y, Li D, Tian P, Shen K, Zhu J, Feng M, et al. The catalase C-262T gene polymorphism and cancer risk: a systematic review and meta-analysis. Medicine (Baltimore) 2015 ;94:e679.
• 21-Corso CR, Acco A. Glutathione system in animal model of solid tumors: From regulation to therapeutic target. Crit Rev Oncol Hematol. 2018;128:43-57.
• 22-Singh Ghalaut V, Kharb S, Ghalaut PS, Rawal A. Lymphocyte glutathione levels in acute leukemia. Clin Chim Acta. 1999;285(1-2):85-9.
• 23-Navarro J, Obrador E, Carretero J, Petschen I, Aviñó J, Perez P, et al. Changes in glutathione status and the antioxidant system in blood and in cancer cells associate with tumour growth in vivo. Free Radic Biol Med. 1999;26(3-4):410-8.
• 24-Jiao Y, Wang Y, Guo S, Wang G. Glutathione peroxidases as oncotargets Oncotarget 2017;8(45):80093-80102.
• 25-Lu YP, Lou YR, Yen P, Newmark HL, Mirochnitchenko OI, Inouye M, et al.Enhanced skin carcinogenesis in transgenic mice with high expression of glutathione peroxidase or both glutathione peroxidase and superoxide dismutase.Cancer Res. 1997;57(8):1468-74.
• 26-Walshe J, Serewko-Auret MM, Teakle N, Cameron S, Minto K, Smith L, et al. Inactivation of glutathione peroxidase activity contributes to UV-induced squamous cell carcinoma formation. Cancer Res. 2007;67(10):4751-8.
• 27-Björkhem-Bergman L, Jönsson K, Eriksson LC, Olsson JM, Lehmann S, Paul C,Björnstedt M. Drug-resistant human lung cancer cells are more sensitive to selenium cytotoxicity. Effects on thioredoxin reductase and glutathione reductase. Biochem Pharmacol. 2002;63(10):1875-84.
• 28-Tisdale MJ, Mahmoud MB. Activities of free radical metabolizing enzymes in tumours. Br J Cancer 1983 Jun;47(6):809-12.
• 29-Pirincci N, Kaba M, Gecit I, Gunes M, Yuksel MB, Tanik S, et al. Serum prolidase activity, oxidative stress, and antioxidant enzyme levels in patients with renal cell carcinoma. Toxicol Ind Health. 2016;32:193e9
• 30-Morabito F, Cristani M, Saija A, Stelitano C, Callea V, Tomaino A, et al. Lipid peroxidation and protein oxidation in patients affected by Hodgkin's lymphoma. Mediators Inflamm. 2004;13(5-6):381-3.
• 31-Kaya E, Keskin L, Aydogdu I, Kuku I, Bayraktar N, Erkut MA. Oxidant/antioxidant parameters and their relationship with chemotherapy in Hodgkin's lymphoma. J Int Med Res. 2005;33(6):687-92.
• 32-Güven M, Oztürk B, Sayal A, Ozet A. Lipid peroxidation and antioxidant system in the blood of patients with Hodgkin's disease. Clin Biochem. 2000;33(3):209-12.
• 33-Seifirad S, Ghaffari A, Amoli MM. The antioxidants dilemma: are they potentially immunosuppressants and carcinogens? Front Physiol. 2014;15:245.