Mavi Yengeçlerden Vibrio parahaemolyticus’un İzolasyonu, İdentifikasyonu ve Bazı Antibiyotiklere Karşı Direnç Profillerinin Belirlenmesi

Yıl 2022, Cilt: 3 Sayı: 1, 1 - 4, 30.06.2022

Sadık Büyükyörük , Meltem Çalışkan Cemil Sahıner

Öz

Bu çalışmanın amacı Aydın İli ve çevresinden temin edilen mavi yengeçlerden Vibrio parahaemolyticus’un izolasyonu, identifikasyonu ile bu suşların antibakteriyel direnç profillerinin belirlenmesidir. İzole edilen altmış adet şüpheli izolattan 4’ü, real-time PCR ile Vibrio parahaemolyticus olarak identifiye edilmiştir. Bu suşların, Penicillin G, clindamycin, piperacilin, amoxicillin-clavulanic acid, ciprofloxacin ve gentamicin gibi antibiyotiklere karşı dirençlilikleri disk difüzyon metodu ile belirlenmiştir. Tüm izolatların penicillin G ve clindamycin’e karşı dirençli olduğu, diğer antibiyotiklere karşı ise değişen düzeylerde duyarlı olduğu tespit edilmiştir.

Anahtar Kelimeler

Mavi yengeç, Vibrio parahaemolyticus, Antibiyotik dirençlilik, Halk sağlığı

Isolation, Identification and Determination of Some Antibiotics Resistance Profiles of Vibrio parahaemolyticus from Blue Crabs

Öz

The purpose of this study is isolation, identification and determination of antibacterial resistance profiles of Vibrio parahaemolyticus from blue crabs obtained from Aydın province. Out of four isolates from sixty suspected isolates were identified as Vibrio parahaemolyticus by real-time PCR. The suspectibility level of these strains to antibiotics, such as Penicillin G, clindamycin, piperacilin, amoxicillin-clavulanic acid, ciprofloxacin and gentamicin was identified with disc diffusion method. It was determined all isolates were resistant to penicillin G and clindamycin and sensitive to other antibiotics at varying levels.

Anahtar Kelimeler

Blue crabs, Vibrio parahaemolyticus, Antibiotic resistance, Public health

Kaynakça

• Referans1. Baron S, Lesne J, Jouy E, Larvor E, Kempf I et al. Antimicrobial susceptibility of autochthonous aquatic Vibrio cholerae in Haiti. Frontiers Microbiology 2016; 7: 1671-1683. doi: 10.3389/fmicb.2016.01671.
• Referans2. Kaya KY ve Yalçın H. Mersin Körfezinde Avlanan Mavi Yengecin (Callinectes sapidus Rathbun, 1896) Mikrobiyolojik Kalitesinin Araştırılması. Türk Tarım – Gıda Bilim ve Teknoloji Dergisi 2018; 6(7): 881-886. doi: 10.24925/turjaf.v6i7.881-886.1858
• Referans3. Maestu AG, Leon AL, Souto RRR, Maneiro RV, Chapela MJ et al. Presence of pathogenic Vibrio species in fresh mussels harvested in the southern Rias of Galicia (NW Spain). Food Control 59 2016; 759-765. doi: 10.1016/j.foodcont.2015.06.054.
• Referans4. Xu YG, Sun LM, Wang YS, Chen PP, Liu ZM et al. Simultaneous detection of Vibrio cholerae, Vibrio alginolyticus, Vibrio parahaemolyticus and Vibrio vulnificus in seafood using dual priming oligonucleotide (DPO) system-based multiplex PCR assay. Food Control 2017; 71: 64-70. doi: 10.1016/j.foodcont.2016.06.024.
• Referans5. Cecchini F, Fajs L, Cosnier S, Marks RS. Vibrio cholerae detection: Traditional assays, novel diagnostic techniques and biosensors. Trends in Analytical Chemistry 2016; 79: 199–209. doi: 10.1016/j.trac.2016.01.017.
• Referans6. Singh A and Barnard TG. Surviving the acid barrier: responses of pathogenic Vibrio cholerae to simulated gastric fluid. Applied Microbiology and Biotechnology 2016; 100: 815–824. doi: 10.1007/s00253-015-7067-2.
• Referans7. Chang HK, Shin YJ, Jang SC, Yu HS, Kim SK et al. Characterization of Vibrio parahaemolyticus isolated from oysters in Korea: Resistance to various antibiotics and prevalence of virulence genes. Marine Pollution Bulletin 2017; 118: 261–266. doi: 10.1016/j.marpolbul.2017.02.070.
• Referans8. Lü CH, Yuan Y, Sun N, Bi Z, Guan B et al. Characterization of Vibrio cholerae isolates from 1976 to 2013 in Shandong Province. Brazillian Journal of Microbiology 2017; 48: 173–179. doi: 10.1016/j.bjm.2016.09.013.
• Referans9. Li B, Chen R, Wang D, Tan H, Ke B et al. Distribution and molecular characteristics of Vibrio cholerae O1 El Tor isolates recovered in Guangdong Province, China, 1961–2013. Infection, Genetics and Evolution 2016; 37: 70–76. doi: 10.1016/j.meegid.2015.11.004.
• Referans10. Yaashikaa PR, Saravanan A, Kumar PS. Isolation and identification of Vibrio cholerae and Vibrio parahaemolyticus from prawn (Penaeus monodon) seafood: Preservation strategies. Microbial Pathogenesis2016; 99: 5-13. doi: 10.1016/j.micpath.2016.07.014.
• Referans11. Eschbach E, Martin A, Huhn J, Seidel J, Heuer R et al. Detection of enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus: performance of real-time PCR kits in an interlaboratory study European Food Reserch Technology 2017; 243: 1335–1342. doi:10.1007/s00217-017-2844-z.
• Referans12. Tan CW, Rukayadi Y, Hasan H, Thung TY, Lee E et al. Prevalence and antibiotic resistance patterns of Vibrio parahaemolyticus isolated from different types of seafood in Selangor, Malaysia. Saudi Journal of Biological Sciences 2020; 27: 1602-1608. doi: 10.1016/j.sjbs.2020.01.002.
• Referans13. Tan CW, Malcolm TTH, Kuan CH, Thung TY, Chang WS et al. Prevalence and Antimicrobial Susceptibility of Vibrio parahaemolyticus Isolated from Short Mackerels (Rastrelliger brachysoma) in Malaysia. Frontiers Microbiology 2017; 8: 1087-1096. doi: 10.3389/fmicb.2017.01087.
• Referans14. CLSI (2010). Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria; Approved Guideline, 3rd Edn. Austin, TX.
• Referans15. CLSI (2016). Performance Standards for Antimicrobial Suceptibility Testing, 26th Edn. CLSI supplement M100S. Wayne, PA: Clinical and Laboratory Standards Institute.
• Referans16. Doğruer Y ve Telli AE. Determination of Vibrio parahaemolyticus in seafoods using direct plate counting, quantitative loop-mediated isothermal amplification and propidium monoazide-qLAMP. Ankara Üniversitesi Veteriner Fakültesi Dergisi 2020; 67: 349-355. doi: 10.33988/auvfd.603868.
• Referans17. Rodgers C, Parveen S, Chigbu P, Jacobs J, Rhodes M et al. Maryland Prevalence of Vibrio parahaemolyticus, and Vibrio vulnificus in blue crabs (Callinectes sapidus), seawater and sediments of the coastal bays. Journal of Applied Microbiology 2014; 117: 1198–1209. doi: 10.1111/jam.12608.
• Referans18. Yalcinkaya F, Ergin C, Agalar C, Kaya S, Aksoylar Y. The presence and antimicrobial susceptibilities of human-pathogen Vibrio spp. isolated from blue crab (Callinectes sapidus) in Belek tourism coast, Turkey. International Journal of Environmental Health Research 2003; 13: 95–98. doi: 10.1080/0960312021000063304.
• Referans19. Parlapani FF, Michailidou S, Anagnostopoulos DA, Koromilas S, Kios K et al. Bacterial communities and potential spoilage markers of whole blue crab (Callinectes sapidus) stored under commercial simulated conditions. Food Microbiology 2019; 82: 325–333. doi: 10.1016/j.fm.2019.03.011.
• Referans20. Sullivan TJ, Neigel JE. Effects of temperature and salinity on prevalence and intensity of infectionof blue crabs, Callinectes sapidus, by Vibrio cholerae, V. parahaemolyticus, and V. vulnificus in Louisiana. Journal of Invertebrate Pathology 2018; 151: 82–90. doi: 10.1016/j.jip.2017.11.004.
• Referans21. Thibodeaux LK, Burnett KG, Burnett LE. Energy metabolism and metabolic depression during exercise in Callinectes sapidus, the Atlantic blue crab: effects of the bacterial pathogen Vibrio campbellii. The Journal of Experimental Biology 2009; 212: 3428-3439. doı: 10.1242/jeb.033431.