Yıl 2020, Cilt 7 , Sayı 2, Sayfalar 680 - 687 2020-12-30

Enzymatic Hydrolysis of Stingray (Dasyatis pastinaca) Muscle and Evaluation of Antioxidant and Copper Chelating Activities of the Hydrolysates
Vatoz (Dasyatis pastinaca) Balığı Kas Dokusunun Enzimatik Hidrolizi ve Hidrolizatların Antioksidan ve Bakır Şelatlama Aktivitelerinin Değerlendirilmesi

Ebru KOCADAĞ KOCAZORBAZ [1] , Meliha KANATSIZOĞLU [2] , Hanife Ttuğçe ÇEKER [3] , Esra MENFAATLİ [4]


Bioactive peptides that originate from different sources, such as plant, animal, fungi, bacteria, and venom, are specific protein fragments that have positive effects on the body functions and health of living organisms. Most of the discovered bioactive peptides from different protein sources showed various biological features, such as antihypertension, antioxidant, antithrombotic, antimicrobial, and anticancer immunomodulation. The aim of this study is the evaluation of biological activities of stingray (Dasyatis pastinaca) muscle tissue protein hydrolysate. Therefore, the homogenized muscle tissue of stingray was hydrolyzed by esperase and trypsin enzymes. Enzyme/substrate ratio, hydrolysis time, and degree were optimized. Moreover, the antioxidant (CUPRAC method) and metal chelating (copper chelating) activities of the hydrolysates were evaluated.
Biyoaktif peptidler canlıların vücut fonksiyonlarına ve sağlığına olumlu etkileri bulunan spesifik protein fragmentleri olup bitkisel, hayvansal, fungal, bakteriyel ve venom kaynaklı olabilmektedir. Farklı protein kaynaklarından keşfedilen biyoaktif peptidlerin çoğu, antihipertansiyon, antioksidan, antitrombotik, antimikrobiyal ve antikanser immünomodülasyon gibi çeşitli biyolojik özellikler göstermiştir. Bu çalışmanın amacı, vatoz (Dasyatis pastinaca) kas dokusu protein hidrolizatının biyolojik aktivitelerinin değerlendirilmesidir. Bu nedenle, vatozun homojenize kas dokusu, esperaz ve tripsin enzimleri tarafından hidrolize edildi. Enzim/substrat oranı, hidroliz süresi ve derecesi optimize edilerek hidrolizatların antioksidan (CUPRAC yöntemi) ve metal şelatlama (bakır şelatlama) aktiviteleri değerlendirildi.
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Birincil Dil en
Konular Temel Bilimler
Bölüm Makaleler
Yazarlar

Orcid: 0000-0001-5611-5235
Yazar: Ebru KOCADAĞ KOCAZORBAZ
Kurum: EGE ÜNİVERSİTESİ
Ülke: Turkey


Orcid: 0000-0001-9547-5444
Yazar: Meliha KANATSIZOĞLU
Kurum: EGE ÜNİVERSİTESİ
Ülke: Turkey


Orcid: 0000-0001-8874-2241
Yazar: Hanife Ttuğçe ÇEKER
Kurum: AKDENİZ ÜNİVERSİTESİ
Ülke: Turkey


Orcid: 0000-0002-2370-6415
Yazar: Esra MENFAATLİ (Sorumlu Yazar)
Kurum: EGE ÜNİVERSİTESİ
Ülke: Turkey


Tarihler

Başvuru Tarihi : 26 Aralık 2019
Kabul Tarihi : 18 Haziran 2020
Yayımlanma Tarihi : 30 Aralık 2020

APA Kocadağ Kocazorbaz, E , Kanatsızoğlu, M , Çeker, H , Menfaatli, E . (2020). Enzymatic Hydrolysis of Stingray (Dasyatis pastinaca) Muscle and Evaluation of Antioxidant and Copper Chelating Activities of the Hydrolysates . Bilecik Şeyh Edebali Üniversitesi Fen Bilimleri Dergisi , 7 (2) , 680-687 . DOI: 10.35193/bseufbd.665435