Chemical Composition and Antibacterial Activity of Three Volatile Oils Extracted from Nigella sativa L. Seeds

Yıl 2023, Cilt: 6 Sayı: 4, 662 - 666, 15.10.2023

Sefa Gözcü , Zeynep Akşit

https://doi.org/10.19127/bshealthscience.1318520

Öz

Nigella sativa L. (Ranunculaceae) and its volatiles have a wide range of benefits. The aim of this study was to investigate the chemical composition and in vitro antibacterial activity of three volatile oils from Erzincan (Local market/Provincial Agriculture and Forestry Office in Erzincan) and Konya. These three samples were grown under different edaphic and climatic conditions. The disc diffusion method was used to test the antibacterial activity against ten standard bacterial strains (Staphylococcus aureus, Clostridium perfringens, Enterococcus faecium, Pseudomonas fluorescens, Pseudomonas aeruginosa, Salmonella enterica, Salmonella enteritidis, Bacillus cereus, Listeria monocytogenes, and Escherichia coli). Monoterpenes were abundant in the chemical composition of all volatile oils tested. The seeds of Erzincan (from local market), Erzincan (from Erzincan Provincial Authority of Agriculture and Forestry) and Konya were characterized by the presence of p-cymene (41.74%-51.98%), α-thujene (16.02%-16.49) and nerol (7.91%-8.50%). Clostridium perfringens (inhibition zone: 35 to 39.3 mm) and Pseudomonas aeruginosa (inhibition zone: 29.7 to 38.7 mm) were found to be particularly sensitive to all volatile oils tested. The results of this study show that the volatile oil of the seeds of N. sativa has remarkable antibacterial activity, which may be due to the presence of various secondary metabolites. In view of the uncontrolled development of antibiotic resistance, these compounds can be used for prophylactic or curative purposes.

Anahtar Kelimeler

Nigella sativa L., Volatile oil, Essential oil, Antibacterial activity

Kaynakça

• Ahmad A, Husain A, Mujeeb M, Khan S A, Najmi A K, Siddique N A, Damanhouri Z A, Anwar F. 2013. A review on therapeutic potential of Nigella sativa: A miracle herb. Asian Pac J Trop Biomed, 3(5): 337-352.
• Aksit H, Bayar Y, Simsek S, Ulutas Y. 2022. Chemical composition and antifungal activities of the essential oils of Thymus species (Thymus pectinatus, Thymus convolutus, Thymus vulgaris) against plant pathogens. J Essent Oil-Bear Plants, 25(1): 200-207.
• Alkis H, Demir E, Taysi M R, Sagir S, Taysi S. 2021. Effects of Nigella sativa oil and thymoquinone on radiation-induced oxidative stress in kidney tissue of rats. Biomed Pharmacother, 139: 111540.
• Almatroudi A, Khadri H, Azam M, Rahmani A H, Al Khaleefah F K, Khateef R, Ansar M A, Allemailem K S. 2020. Antibacterial, antibiofilm and anticancer activity of biologically synthesized silver nanoparticles using seed extract of Nigella sativa. Processes, 8(4): 388.
• Bakal S N, Bereswill S, Heimesaat M M. 2017. Finding novel antibiotic substances from medicinal plants—antimicrobial properties of Nigella sativa directed against multidrug resistant bacteria. Eur J Microbiol Immunol, 7(1): 92-98.
• Burits M, Bucar F. 2000. Antioxidant activity of Nigella sativa essential oil. Phytother Res,14(5): 323-328.
• Cristani M, D'Arrigo M, Mandalari G, Castelli F, Sarpietro M G, Micieli D, Trombetta D. 2007. Interaction of four monoterpenes contained in essential oils with model membranes: implications for their antibacterial activity. J Agric Food Chem, 55(15): 6300-6308
• Daoudi H, Bouafia A, Meneceur S, Laouini S E, Belkhalfa H, Lebbihi R, Selmi B. 2022. Secondary metabolite from nigella sativa seeds mediated synthesis of silver oxide nanoparticles for efficient antioxidant and antibacterial activity. J Inorg Organomet Polym Mater, 32(11): 4223-4236.
• Erdoğan Ü, Özmen Ö, Özer M. 2023. Wound Healing, Anti-analgesic, and Antioxidant Activity of Nigella sativa Linn., Essential Based Topical Formulations in Rat Model Experimental Skin Defects. J Essent Oil-Bear Plants, 26(1): 45-60.
• Hossain M S, Sharfaraz A, Dutta A, Ahsan A, Masud M A, Ahmed I A, Goh B H, Urbi Z, Sarker M M R, Ming L C. 2021. A review of ethnobotany, phytochemistry, antimicrobial pharmacology and toxicology of Nigella sativa L. Biomed Pharmacother, 143: 112182.
• Joshi B, Kumar V, Chandra B, Kandpal N. 2019. Chemical composition and antibacterial activity of essential oil of Senecio graciliflorus. J Drug Deliv Ther, 9: 98-100.
• Kazemi M. 2014. Phytochemical composition, antioxidant, anti-inflammatory and antimicrobial activity of Nigella sativa L. essential oil. J Essent Oil-Bear, 17(5): 1002-1011.
• Khazdair M R, Gholamnezhad Z, Rezaee R, Boskabady M H. 2021. A qualitative and quantitative comparison of Crocus sativus and Nigella sativa immunomodulatory effects. Biomed Pharmacother, 140: 111774.
• Marchese A, Arciola C R, Barbieri R, Silva A S, Nabavi S F, Tsetegho Sokeng A J, Izadi M, Jafari N J, Suntar I, Daglia M. 2017. Update on monoterpenes as antimicrobial agents: A particular focus on p-cymene. Materials, 10(8): 947.
• Mehalaine S, Chenchouni H. 2021. Quantifying how climatic factors influence essential oil yield in wild-growing plants. Arab J Geosci, 14(13): 1257.
• Mozaffari F, Ghorbanli M, Babai A, Sepehr M F. 2000. The effect of water stress on the seed oil of Nigella sativa L. J Essent Oil Res, 12(1): 36-38.
• Omidi H, Khorram S, Mesgari M, Asghari-Jafarabadi M, Tarighat-Esfanjani A. 2017. Effects of separate and concurrent supplementation of Nano-sized clinoptilolite and Nigella sativa on oxidative stress, anti-oxidative parameters and body weight in rats with type 2 diabetes. Biomed Pharmacother, 96: 1335-1340.
• Salman M T, Khan R A, Shukla I. 2016. Antibacterial activity of Nigella Sativa Linn. seeds against multiple antibiotics resistant clinical strains of Staphylococcus aureus. IABCR, 2(3): 96-99.
• Shrivastava S R, Shrivastava P S, Ramasamy J. 2018. World health organization releases global priority list of antibiotic-resistant bacteria to guide research, discovery, and development of new antibiotics. J Med Soc, 32(1): 76.
• Tariq S, Wani S, Rasool W, Shafi K, Bhat M A, Prabhakar A, Shalla A H, Rather M A. 2019. A comprehensive review of the antibacterial, antifungal and antiviral potential of essential oils and their chemical constituents against drug-resistant microbial pathogens. Microb Pathog, 134: 103580.
• Ugur A R, Dagi H T, Ozturk B, Tekin, G, Findik D. 2016. Assessment of in vitro antibacterial activity and cytotoxicity effect of Nigella sativa oil. Pharmacogn Mag, 12: 471-474.
• Wajs A, Bonikowski R, Kalemba D. 2008. Composition of essential oil from seeds of Nigella sativa L. cultivated in Poland. Flavour Fragr J, 23(2): 126-132.
• Wayne P A. 1999. Performance standards for antimicrobial susceptibility testing. 9th International Supplement, M100-S9. National Committee for Clinical Laboratory Standards, New York, USA, pp: 332.
• Yang F. Chen L, Zhao D, Guo T, Yu D, Zhang X, Li P, Chen J. 2023. A novel water-soluble chitosan grafted with nerol: Synthesis, characterization and biological activity. Int J Biol Macromol, 232: 123498.
• Yarnell E, Abascal K. 2011. Nigella sativa: holy herb of the middle East. Altern Complement Ther, 17(2): 99-105.