İn vitro şartlarda Yetiştirilen Buttum (Pistacia khinjuk Stocks)’da Çözünür Karbonhidrat Değerleri ile Antioksidan Peroksidaz Aktivitesi üzerine Tuz Stresinin Etkileri
Bu
çalışmada, in vitro koşullarda kültüre alınan Buttum (Pistacia khinjuk Stocks) bitkisine ait
olgun tohumlar farklı tuz (NaCl) parametrelerine (0, 50, 100, 150, 200, 250 mM)tabi tutulmuştur. Farklı NaCl
konsantrasyonlarını içeren ve hormonsuz MS (Murashige ve Skoog, 1962) besi
ortamında çimlendirilen tohumlar, 4 hafta boyunca tuz stresine maruz bırakılmıştır. Kültür
sonunda elde edilen fidelerin kök ve yapraklarında bulunan
çözünür karbonhidrat değerleri (Glukoz ve Fruktoz) ile yapraklarında bulunan peroksidaz
enzim (POD) aktiviteleri araştırılmıştır. İnceleme sonucunda artan NaCl konsantrasyonuna bağlı olarak hem çözünür
karbonhidrat değerlerinde, hem de POD aktivitelerinde pozitif bir korelasyon olduğu
tespit edilmiştir.
Abbaspour, H., Afshari, H., ve Abdel-Wahhab, M.A. (2012). Influence of salt stress on growth, pigments, soluble sugars and ion accumulation in three pistachio cultivars. Journal of Medicinal Plants Research, 6 (12), 2468-2473.
Adish, M., Fekri. M., ve Hokmabadi, H. (2010). Response of Badami-Zarand Pistachio Rootstock to Salinity Stress. Int J Nuts Related Sci, 1, 1-111.
Al-Saghir, M.G., ve Porter, D.M. (2012). Taxonomic Revision of the Genus Pistacia L. (Anacardiaceae). AJPS, 3, 12-32.
Ardakani, M.J. (2015). Effect of salinity on germination and deployment of Pistachio vera var. Badami. European Journal of Experimental Biology, 5 (4), 57-59.
Asadollahi, Z., ve Mozaffari, V. (2013). Effects of salinity and manganese on growth and chemical composition of pistachio (Pistacia vera L.) seedlings in perlite medium. EJGCST, 3 (4), 13-28.
Ben hamed, S., ve Lefi, E. (2015). Dynamics of growth and phytomass allocation in seedlings of Pistacia atlantica Desf. versus Pistacia vera L. under salt stress. IJAAR, 1 (6), 16-27.
Benhassaini, H., Fetati, A., Hocine, A.K., ve Belkhodja, M. (2012). Effect of salt stress on growth and accumulation of proline and soluble sugars on plantlets of Pistacia atlantica Desf. subsp. atlantica used as rootstocks. Biotechnol Agron Soc Environ, 16, 159-165.
Bozorgi, M., Memariani, Z., Mobli, M., Salehi Surmaghi, M.H., Shams-Ardekani, M.R., ve Rahimi, R. (2013). Five Pistacia species (P. vera, P. atlantica, P. terebinthus, P. khinjuk, and P. lentiscus): A Review of Their Traditional Uses, Phytochemistry, and Pharmacology. The Scientific World Journal, 219815, 1- 33.
Chelli-Chaabouni, A., Mosbahb, A.B., Maalej, M., Gargouric, K., Gargouri-Bouzidd, R., ve Drira N. (2010). In vitro salinity tolerance of two pistachio rootstocks: Pistacia vera L. and P. atlantica Desf. Environ Exp Bot, 69, 302-312.
Cristiano, G., Camposeo, S., Fracchiolla, M., Vivaldi, G.A., De Lucia B., ve Cazzato, E. (2016). Salinity differentially affects growth and ecophysiology of two mastic tree (Pistacia lentiscus L.) accessions. Forests, 7 (8), 156.
Çulha, Ş., ve Çakırlar, H. (2012). Tuzluluğun bitkiler üzerine etkileri ve tuz tolerans mekanizmaları, Afyon Kocatepe Üniversitesi Fen Bilimleri Dergisi, 11, 11-34.
Ekmekçi, E., Apan, M., ve Kara T. (2005). Tuzluluğun bitki gelişimine etkisi. OMÜ Zir Fak Dergisi, 20, 118-125.
Feng, L. (2011). Comparative Study of Slat Resistance of Pistacia chinensis and Hovenia dulcis Journal of Northwest Forestry University, 3, 41-44.
Gupta, B., ve Huang, B. (2014). Mechanism of Salinity Tolerance in Plants: Physiological, Biochemical, and Molecular Characterization. International Journal of Genomics, 214, 1-18.
Halhoul., M.N., ve Kleinberg., I. (1972). Differential Determination of Glucose and Fructose Yielding Substances with Anthrone. Anal. Biochem, 50, 337-343.
Kumar, K.B., ve Khan, P.A. (1982). Peroxidase and polyphenol oxidase in excised ragi (Eleusine coracana cv. PR 202) leaves during senescence. Ind J Exp Bot, 20, 412-416.
Kumar, V., Shriram, V., Nikam, T.D., Jawali, N., ve Shitole, M.G. (2009). Antioxidant enzyme activities and protein profiling under salt stress in indica rice genotypes differing in salt tolerance. Arch Agron Soil Sci, 55, 379-394.
Lotfi, A., Jahanbakhshian, Z., Faghihi, F., Seyedi Mahdi., S. (2015). The effect of salinity stress on survival percentage and physiological characteristics in three varieties of pistachio (Pistacia vera). Biologia, 70 (9), 1185-1192.
M'barek, B.N., Cheick-M'hamed, H., Raoudha, A., ve Bettaib-Kaab, L. (2007). Relationship Between Peroxidase Activity and Salt Tolerance During Barley Seed Germination. Agron J, 6, 433-438.
Mirzaei, J., ve Yousefzadeh, H. (2013). Peroxidase, superoxide dismutase and catalase activities of the Pistacia khinjuk seedlings under drought stress. Ecopersia, 1, 329-337.
Murashige, T., ve Skoog, F.A. (1962). Revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant, 15, 473-97.
Ranjbarfordoei, A., Samson, R., Lemeur, R. ve Van Damme P. (2002) Effects of Osmotic Drought Stress Induced by a Combination of NaCl and Polyethylene Glycol on Leaf Water Status, Photosynthetic Gas Exchange, and Water Use Efficiency of Pistacia khinjuk and P. mutica. Photosynthetica, 40, 165-169.
Shahraji, T.R., Hajimerzai ve Shabaian, N. (2010). Physiological responses of Pistacia khinjuk (Stocks) seedlings to water stress. International Journal of Biological Technology, 1 (2), 44-49.
Tavallali, V., Rahemi, M., ve Panahi, B. (2008). Calcium induces salinity tolerance in pistachio rootstocks. Fruits, 63, 285-296.
Tilkat, E., Işıkalan, Ç., ve Onay, A., (2005), In vitro Propagation of Khinjuk Pistachio (Pistacia khinjuk Stocks) Through Seedling Apical Shoot Tip Culture, Propagation of Ornamental Plants, 5 (3), 124-128.
Weisany, W., Sohrabi, Y., Heidari, G., Siosemardeh, A., ve Ghassemi-Golezani, K. (2012). Changes in antioxidant enzymes activity and plant performance by salinity stress and zinc application in soybean (Glycine max L.). Plant Omics J, 5, 60- 67.
Yıldız, M., Terzi, H., Cenkci, S.E., Arıkan Terzi, E.S., ve Uruşak, B. (2010). Physiological and Biochemical Markers of Salinity Tolerance in Plants. Anadolu Unıversıty Journal Of Science And Technology, 1, 1-33.
Zarinkamar, F., ve Farjady, E. (2011). Salt’s Effect on Anatomy and Deformity of Pistacia vera L. Nut. J Agric Sci Technol, 1, 599-607.
Abbaspour, H., Afshari, H., ve Abdel-Wahhab, M.A. (2012). Influence of salt stress on growth, pigments, soluble sugars and ion accumulation in three pistachio cultivars. Journal of Medicinal Plants Research, 6 (12), 2468-2473.
Adish, M., Fekri. M., ve Hokmabadi, H. (2010). Response of Badami-Zarand Pistachio Rootstock to Salinity Stress. Int J Nuts Related Sci, 1, 1-111.
Al-Saghir, M.G., ve Porter, D.M. (2012). Taxonomic Revision of the Genus Pistacia L. (Anacardiaceae). AJPS, 3, 12-32.
Ardakani, M.J. (2015). Effect of salinity on germination and deployment of Pistachio vera var. Badami. European Journal of Experimental Biology, 5 (4), 57-59.
Asadollahi, Z., ve Mozaffari, V. (2013). Effects of salinity and manganese on growth and chemical composition of pistachio (Pistacia vera L.) seedlings in perlite medium. EJGCST, 3 (4), 13-28.
Ben hamed, S., ve Lefi, E. (2015). Dynamics of growth and phytomass allocation in seedlings of Pistacia atlantica Desf. versus Pistacia vera L. under salt stress. IJAAR, 1 (6), 16-27.
Benhassaini, H., Fetati, A., Hocine, A.K., ve Belkhodja, M. (2012). Effect of salt stress on growth and accumulation of proline and soluble sugars on plantlets of Pistacia atlantica Desf. subsp. atlantica used as rootstocks. Biotechnol Agron Soc Environ, 16, 159-165.
Bozorgi, M., Memariani, Z., Mobli, M., Salehi Surmaghi, M.H., Shams-Ardekani, M.R., ve Rahimi, R. (2013). Five Pistacia species (P. vera, P. atlantica, P. terebinthus, P. khinjuk, and P. lentiscus): A Review of Their Traditional Uses, Phytochemistry, and Pharmacology. The Scientific World Journal, 219815, 1- 33.
Chelli-Chaabouni, A., Mosbahb, A.B., Maalej, M., Gargouric, K., Gargouri-Bouzidd, R., ve Drira N. (2010). In vitro salinity tolerance of two pistachio rootstocks: Pistacia vera L. and P. atlantica Desf. Environ Exp Bot, 69, 302-312.
Cristiano, G., Camposeo, S., Fracchiolla, M., Vivaldi, G.A., De Lucia B., ve Cazzato, E. (2016). Salinity differentially affects growth and ecophysiology of two mastic tree (Pistacia lentiscus L.) accessions. Forests, 7 (8), 156.
Çulha, Ş., ve Çakırlar, H. (2012). Tuzluluğun bitkiler üzerine etkileri ve tuz tolerans mekanizmaları, Afyon Kocatepe Üniversitesi Fen Bilimleri Dergisi, 11, 11-34.
Ekmekçi, E., Apan, M., ve Kara T. (2005). Tuzluluğun bitki gelişimine etkisi. OMÜ Zir Fak Dergisi, 20, 118-125.
Feng, L. (2011). Comparative Study of Slat Resistance of Pistacia chinensis and Hovenia dulcis Journal of Northwest Forestry University, 3, 41-44.
Gupta, B., ve Huang, B. (2014). Mechanism of Salinity Tolerance in Plants: Physiological, Biochemical, and Molecular Characterization. International Journal of Genomics, 214, 1-18.
Halhoul., M.N., ve Kleinberg., I. (1972). Differential Determination of Glucose and Fructose Yielding Substances with Anthrone. Anal. Biochem, 50, 337-343.
Kumar, K.B., ve Khan, P.A. (1982). Peroxidase and polyphenol oxidase in excised ragi (Eleusine coracana cv. PR 202) leaves during senescence. Ind J Exp Bot, 20, 412-416.
Kumar, V., Shriram, V., Nikam, T.D., Jawali, N., ve Shitole, M.G. (2009). Antioxidant enzyme activities and protein profiling under salt stress in indica rice genotypes differing in salt tolerance. Arch Agron Soil Sci, 55, 379-394.
Lotfi, A., Jahanbakhshian, Z., Faghihi, F., Seyedi Mahdi., S. (2015). The effect of salinity stress on survival percentage and physiological characteristics in three varieties of pistachio (Pistacia vera). Biologia, 70 (9), 1185-1192.
M'barek, B.N., Cheick-M'hamed, H., Raoudha, A., ve Bettaib-Kaab, L. (2007). Relationship Between Peroxidase Activity and Salt Tolerance During Barley Seed Germination. Agron J, 6, 433-438.
Mirzaei, J., ve Yousefzadeh, H. (2013). Peroxidase, superoxide dismutase and catalase activities of the Pistacia khinjuk seedlings under drought stress. Ecopersia, 1, 329-337.
Murashige, T., ve Skoog, F.A. (1962). Revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant, 15, 473-97.
Ranjbarfordoei, A., Samson, R., Lemeur, R. ve Van Damme P. (2002) Effects of Osmotic Drought Stress Induced by a Combination of NaCl and Polyethylene Glycol on Leaf Water Status, Photosynthetic Gas Exchange, and Water Use Efficiency of Pistacia khinjuk and P. mutica. Photosynthetica, 40, 165-169.
Shahraji, T.R., Hajimerzai ve Shabaian, N. (2010). Physiological responses of Pistacia khinjuk (Stocks) seedlings to water stress. International Journal of Biological Technology, 1 (2), 44-49.
Tavallali, V., Rahemi, M., ve Panahi, B. (2008). Calcium induces salinity tolerance in pistachio rootstocks. Fruits, 63, 285-296.
Tilkat, E., Işıkalan, Ç., ve Onay, A., (2005), In vitro Propagation of Khinjuk Pistachio (Pistacia khinjuk Stocks) Through Seedling Apical Shoot Tip Culture, Propagation of Ornamental Plants, 5 (3), 124-128.
Weisany, W., Sohrabi, Y., Heidari, G., Siosemardeh, A., ve Ghassemi-Golezani, K. (2012). Changes in antioxidant enzymes activity and plant performance by salinity stress and zinc application in soybean (Glycine max L.). Plant Omics J, 5, 60- 67.
Yıldız, M., Terzi, H., Cenkci, S.E., Arıkan Terzi, E.S., ve Uruşak, B. (2010). Physiological and Biochemical Markers of Salinity Tolerance in Plants. Anadolu Unıversıty Journal Of Science And Technology, 1, 1-33.
Zarinkamar, F., ve Farjady, E. (2011). Salt’s Effect on Anatomy and Deformity of Pistacia vera L. Nut. J Agric Sci Technol, 1, 599-607.
Ayaz Tilkat, E., Kaplan, A., Hoşer, A., Tilkat, E. (2017). İn vitro şartlarda Yetiştirilen Buttum (Pistacia khinjuk Stocks)’da Çözünür Karbonhidrat Değerleri ile Antioksidan Peroksidaz Aktivitesi üzerine Tuz Stresinin Etkileri. Batman Üniversitesi Yaşam Bilimleri Dergisi, 7(2/2), 90-97.