Does Exogenously Applied Gallic Acid Regulate the Enzymatic and Non-Enzymatic Antioxidants in Wheat Roots Exposed to Cadmium Stress?

Öz

The aim of the current study was to determine whether gallic acid (GLA) triggers the growth, osmoregulation and antioxidant system related to defense mechanisms in wheat roots to cadmium (Cd)-induced oxidative stress. For this purpose, wheat plants were hydroponically grown for 21 (d) and were treated with GLA (GLA1-2; 25 and 75 mM), Cd stress (Cd1-2; 100 and 200 mM) and their combination for 7 d. The significant reduction in growth (RGR) and osmotic potential (Y_P) was observed under stress. After GLA applications in response to stress, RGR, Y_P and proline (Pro) increased, except for 200 mM Cd plus 75 mM GLA. Under stress, hydrogen peroxide (H₂O₂) was induced by the activated superoxide dismutase (SOD) activity but, NADPH oxidase (NOX) had no contribution on the accumulation of H₂O₂. Despite of the increased catalase (CAT) and glutathione reductase (GR), H₂O₂ did not eliminate and then lipid peroxidation (TBARS content) was induced with the decreased scavenging capacity of hydroxyl radical (OH^·) under stress. Besides, to remove of H₂O₂ content produced by SOD, H₂O₂ could effectively scavenge through CAT activity in combination form of GLA and Cd. On the other hand, GLA did not induce the enzymes and non-enzymes related to Asada-Halliwell cycle (ascorbate peroxidase (APX), GR, dehydroascorbate reductase (DHAR), monodehydroascorbate reductase (MDHAR), reduced and oxidized contents of glutathione (GSH and GSSG contents). Under high Cd concentration, GLA2 could not eliminate H₂O₂ content because of increased NOX activity and then in this group (Cd2+GLA2) the scavenging capacity of OH^· did not change and TBARS content increased. 

Anahtar Kelimeler

• Antioxidant enzymes,Asada-Halliwell pathway,Cadmium,Gallic acid,Lipid peroxidation

Kaynakça

1. 1. Asgher, M, Khan, NA, Khan, MIR, Fatma, M, Masood A. 2014. Ethylene production is associated with a-lleviation of cadmium-induced oxidative stress by sulfur in mustard types differing in ethylene sensitivity. Ecotoxicology and Environmental Safety; 106: 54-61.
2. 2. Roychoudhury, A, Basu, S, Sengupta, DN. 2012. Antioxidants and stress-related metabolites in the seedlings of two indica rice varieties exposed to cadmium chloride toxicity. Acta Physiologica Plantarum; 34: 835–847.
3. 3. Srivastava, AK, Srivastava, S, Mishra, S, Prasanna, P, D’Souza, SF. 2014. Identification of redox-regulated components of arsenate (AsV) tolerance through thiourea supplementation in rice. Metallomics; 6: 1718–1730.
4. 4. Kim, Y, Mun, BG, Wagas, M, Kim HH, Shahzad, R, Imran, M, Yun, BW, Lee, IJ. 2018. Regulation of reactive oxygen and nitrogen species by salicylic acid in rice plants under salinity stress conditions. Plos one; 13(3): 1-20.
5. 5. El-Soud, WA, Hegab, MM, AbdElgawad, H, Zinta, G, Asard, H. 2013. Ability of ellagic acid to alleviate osmotic stress on chickpea seedlings. Plant Physiology Biochemistry; 271: 173–183.
6. 6. Michalak, A. 2006. Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Polish Journal of Environmental Studies; 15: 523-530.
7. 7. Kamdem, JP, Stefanello, ST, Boligon, AA, Wagner, C, Kade, IJ, Pereira, RP, Preste, AD, Roos, DH, Waczuk, EP, Appel, AS, Athayde, Ml, Souza, DO, Rocha. JBT. 2012. In vitro antioxidant activity of stem bark of Trichilia catigua Adr. Juss. Acta Pharmaceutica; 62: 371-382.
8. 8. Ozfidan-Konakci, C., Yildiztugay, E., Kucukoduk, M. 2015. Protective roles of exogenously applied gallic acid in Oryza sativa subjected to salt andosmotic stresses: effects on the total antioxidant capacity. Plant Growth Regulation; 75(1): 219–234.

9. 9. Yildiztugay, E, Ozfidan-Konakci, C, Kucukoduk, M. 2017. Improvement of cold stress resistance via free radical scavenging ability and promoted water status and photosynthetic capacity of gallic acid in soybean leaves. Journal of Soil Science and Plant Nutrition; 17 (2): 366-384.
10. 10. Breiman, A, Graur, B. 1995. Wheat Evolution. Israel Journal of Plant Science; 43: 85-98.
11. 11. Singh, R, Parihar, P, Singh, M, Bajguz, A, Kumar, J, Singh, S, Singh, VP, Prasad, SM. 2017. Uncovering potential applications of Cyanobacteria and algal metabolites in biology, agriculture and medicine: current status and future prospects. Frontiers in Microbiology; 8, 515.
12. 12. Hussain, MI, Gonzalez, L, Reigosa, MJ. 2011. Allelopathic potential of Acacia melanoxylon R. Br. on the germination and root growth of native species. Weed Biolology and Management; 11: 18–28.
13. 13. Mattioli, R., Falasca, G., Sabatini, S., Altamura, M.M., Costantino, P., Trovato, M. 2009. The proline biosynthetic genes P5CS1 and P5CS2 play overlapping roles in Arabidopsis flower transition but not in embryo development. Physiologia Plantarum; 137, 72–85.
14. 14. Guo, J, Shiyu, Q, Rengel, Z, Gao, W, Nie, Z, Hongen, L, Chang, L, Zhoa, P. 2019. Cadmium stress increases antioxidant enzyme activities and decreases endogenous hormone concentrations more in Cd-tolerant than Cd-sensitive wheat varieties. Ecotoxicology and Environmental Safety; 172: 380-387.
15. 15. Pan, C, Haoliang, L, Jinfeng, Y, Yumei, L, Chonling, Y. 2019. Identification of Cadmium-responsive Kandelia obovata SOD family genes and response to Cd toxicity. Environmental and Experimental Botany; 162: 230-238.
16. 16. Bhardwaj, R, Kaur, L, Srivastava, P. 2017. Comparative Evaluation of Different Phenolic Acids as Priming Agents for Mitigating Drought Stress in Wheat Seedlings. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences; 87(4): 1133-1142.
17. 17. Keunen, E, Peshev, D, Vangronsveld, J, Van Den Ende, W, Cuypers, A. 2013. Plant sugars are crucial players in the oxidative challenge during abiotic stress: extending the traditional concept. Plant Cell and Environment; 36: 1242-1255.
18. 18. Rahman, A, Hossain, S, Mahmud, J, Nahar, K, Hasanuzzaman, M, Fujita, M. 2016. Manganese-induced salt stress tolerance in rice seedlings: regulation of ion homeostasis, antioxidant defense and glyoxalase systems. Physiology and Molecular Biology of Plants; 22(3): 291-306.
19. 19. Li, Q., Yu, B., Gao, Y., Dai, A.H., Bai, J.G. 2011. Cinnamic acid pretreatment mitigates chilling stress of cucumber leaves through altering antioxidant enzyme activity. Journal of Plant Physiology; 168: 927–934.
20. 20. Dai, A.H., Nie, Y.X., Yu, B., Li, Q., Lu, L.Y., Bai, J.G. 2012. Cinnamic acid pretreatment enhances heat tolerance of cucumber leaves through modulating antioxidant enzyme activity. Environmental and Experimental Botany; 79: 1–10.