In Vitro Propagation of Wetland Medicinal Plant Lythrum salicaria L.

Yıl 2018, Cilt: 14 Sayı: 4, 369 - 372, 28.12.2018

Betül Akın Burcu Çetin Nüket Akanıl Bingöl

https://doi.org/10.18466/cbayarfbe.393626

Cited By: 1

Öz

The aim of this experiment is to develop a protocol to establish the in vitro propagation potential of Lythrum salicaria L (purple loosestrife).
Seeds of L. salicaria were germinated
and grown in Murashige Skoog (MS) medium for 30 days. At the end of 30 days, shoot tips of L. salicaria were cultured on MS medium supplemented with different
combinations and concentrations of 6-benzylaminopurine (BAP; 1, 2 and 3 mg/L) and
1-naphthaleneacetic acid (NAA; 0, 0.1 and 0.5 mg/L). Regenerated shoots were transferred
to MS supplemented with varying concentrations of different auxins: NAA (0.5
and 1 mg/L) and indole-3-butyric acid (IBA; 0.5 and 1.0 mg/L) for rooting. Among
the cytokinins investigated, BAP indicated the best response to initiate shoot
elongation and multiple shoot formation alone. The highest number of shoot per
explant (37±3.38) was obtained in MS medium with 1 mg/L BAP. Although, the
highest shoot length (3.1 cm ± 0.48) was found on medium with 1 mg/L BAP+0.5 mg/L
NAA, there were no statistically differences among all types of medium.
Percentage of rooting, shoot length and root length were altered significantly
with different concentrations of NAA and IBA. Thus, shoot length (9.5 cm±0.46),
root length (4.1 cm±0.13) and the percentage of rooting (100%) increased
significantly with a concentration of 1 mg/L NAA.  It was concluded that MS medium with 1 mg/L
NAA has been superior for root development compared to other hormone
concentrations.

Anahtar Kelimeler

auxins, cytokinins, purple loosestrife, rooting, tissue culture

Kaynakça

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