Yıl 2020, Cilt 16 , Sayı 1, Sayfalar 55 - 59 2020-03-27

Cytotoxic Effects of Probiotic Bacteria Lactobacillus acidophilus and Lactobacillus rhamnosus Grown in the Presence of Oleuropein on Human Prostate Cancer Cells

Hasan Ufuk CELEBİOGLU [1] , Busenur ÇELEBİ [2] , Yavuz ERDEN [3] , Emre EVİN [4] , Orhan ADALI [5]


Functional foods are groups of foods that have potentially positive effects on health beyond basic nutrition. Probiotics are beneficial microorganisms, being as a group of functional foods, and when ingested in adequate amounts, they possess positive health effects to host organisms. Lactobacillus acidophilus and Lactobacillus rhamnosus are two of the most studied and very well-known probiotic bacteria. They can have anti-carcinogenic functions, yet their exact mechanisms how they possess anti-carcinogenic activities are still under investigation.

Another group of functional foods is plant-based phenolic compounds. They also have potential to possess anti-carcinogenic activities. Among them, oleuropein is very interesting and well-known phenolic compound naturally present in olive fruits and olive oil.

As the phenolic compounds and probiotic bacteria can be present at the same time in human gastrointestinal tract, this study aims to investigate the in vitro cytotoxic effects of Lactobacillus acidophilus LA-5 (LA-5) and Lactobacillus rhamnosus GG (GG) grown in the presence of oleuropein on human prostate cancer cell line. To achieve this purpose, oleuropein was added to media of these bacteria and effects of cell-free supernatants of these combinations (phenolic+probiotic) on cytotoxicity of prostate cancer cell line PC-3 were investigated using MTT Method. 

Concentrations of 0, 50, 100, and 250 µg/mL of oleuropein were added to bacterial cultures and no inhibition on growths of LA-5 or GG were observed. Cell surface hydrophobicity (i.e., adhesion to hydrophobic solvents) is an important feature for bacteria to be retained in gastrointestinal tract (GIT). MATS tests showed all concentrations of oleuropein decreased the surface hydrophobicity of GG, while a dose-dependent increase or decrease in surface hydrophobicity was observed in LA-5. Bacterial aggregation is defined as clustering of bacteria. Auto-aggregation studies showed oleuropein affected the aggregation of bacteria in a dose-dependent manner. Oleuropein added to the growth medium of LA-5 had additional effects on the cytotoxicity of cell-free supernatants of this bacterium. However, addition of oleuropein to the growth medium of GG did not show significant changes on the cytotoxicity of cell-free supernatants of this bacterium.

Thus, the results indicate that new combinations of functional foods have potential to formulate new nutraceuticals. 

Cytotoxicity, MTT Assay, phenolic compounds, probiotics, prostate cancer, oleuropein
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Birincil Dil en
Bölüm Makaleler
Yazarlar

Yazar: Hasan Ufuk CELEBİOGLU (Sorumlu Yazar)
Kurum: BARTIN ÜNİVERSİTESİ, FEN FAKÜLTESİ, BİYOTEKNOLOJİ BÖLÜMÜ
Ülke: Turkey


Orcid: 0000-0001-9774-6068
Yazar: Busenur ÇELEBİ
Kurum: BARTIN ÜNİVERSİTESİ, FEN FAKÜLTESİ, BİYOTEKNOLOJİ BÖLÜMÜ
Ülke: Turkey


Orcid: 0000-0002-2807-6096
Yazar: Yavuz ERDEN
Kurum: BARTIN ÜNİVERSİTESİ, FEN FAKÜLTESİ, MOLEKÜLER BİYOLOJİ VE GENETİK BÖLÜMÜ
Ülke: Turkey


Orcid: 0000-0002-5805-7419
Yazar: Emre EVİN
Kurum: Middle East Technical University, Faculty of Science and Arts, Department of Biological Sciences
Ülke: Turkey


Orcid: 0000-0001-8484-1146
Yazar: Orhan ADALI
Kurum: Middle East Technical University, Faculty of Science and Arts, Department of Biological Sciences
Ülke: Turkey


Teşekkür Chr. Hansen, Turkey is acknowledged for obtaining the probiotic strains.
Tarihler

Yayımlanma Tarihi : 27 Mart 2020

Bibtex @araştırma makalesi { cbayarfbe633237, journal = {Celal Bayar University Journal of Science}, issn = {1305-130X}, eissn = {1305-1385}, address = {}, publisher = {Celal Bayar Üniversitesi}, year = {2020}, volume = {16}, pages = {55 - 59}, doi = {}, title = {Cytotoxic Effects of Probiotic Bacteria Lactobacillus acidophilus and Lactobacillus rhamnosus Grown in the Presence of Oleuropein on Human Prostate Cancer Cells}, key = {cite}, author = {CELEBİOGLU, Hasan Ufuk and ÇELEBİ, Busenur and ERDEN, Yavuz and EVİN, Emre and ADALI, Orhan} }
APA CELEBİOGLU, H , ÇELEBİ, B , ERDEN, Y , EVİN, E , ADALI, O . (2020). Cytotoxic Effects of Probiotic Bacteria Lactobacillus acidophilus and Lactobacillus rhamnosus Grown in the Presence of Oleuropein on Human Prostate Cancer Cells. Celal Bayar University Journal of Science , 16 (1) , 55-59 . Retrieved from https://dergipark.org.tr/tr/pub/cbayarfbe/issue/53288/633237
MLA CELEBİOGLU, H , ÇELEBİ, B , ERDEN, Y , EVİN, E , ADALI, O . "Cytotoxic Effects of Probiotic Bacteria Lactobacillus acidophilus and Lactobacillus rhamnosus Grown in the Presence of Oleuropein on Human Prostate Cancer Cells". Celal Bayar University Journal of Science 16 (2020 ): 55-59 <https://dergipark.org.tr/tr/pub/cbayarfbe/issue/53288/633237>
Chicago CELEBİOGLU, H , ÇELEBİ, B , ERDEN, Y , EVİN, E , ADALI, O . "Cytotoxic Effects of Probiotic Bacteria Lactobacillus acidophilus and Lactobacillus rhamnosus Grown in the Presence of Oleuropein on Human Prostate Cancer Cells". Celal Bayar University Journal of Science 16 (2020 ): 55-59
RIS TY - JOUR T1 - Cytotoxic Effects of Probiotic Bacteria Lactobacillus acidophilus and Lactobacillus rhamnosus Grown in the Presence of Oleuropein on Human Prostate Cancer Cells AU - Hasan Ufuk CELEBİOGLU , Busenur ÇELEBİ , Yavuz ERDEN , Emre EVİN , Orhan ADALI Y1 - 2020 PY - 2020 N1 - DO - T2 - Celal Bayar University Journal of Science JF - Journal JO - JOR SP - 55 EP - 59 VL - 16 IS - 1 SN - 1305-130X-1305-1385 M3 - UR - Y2 - 2020 ER -
EndNote %0 Celal Bayar Üniversitesi Fen Bilimleri Dergisi Cytotoxic Effects of Probiotic Bacteria Lactobacillus acidophilus and Lactobacillus rhamnosus Grown in the Presence of Oleuropein on Human Prostate Cancer Cells %A Hasan Ufuk CELEBİOGLU , Busenur ÇELEBİ , Yavuz ERDEN , Emre EVİN , Orhan ADALI %T Cytotoxic Effects of Probiotic Bacteria Lactobacillus acidophilus and Lactobacillus rhamnosus Grown in the Presence of Oleuropein on Human Prostate Cancer Cells %D 2020 %J Celal Bayar University Journal of Science %P 1305-130X-1305-1385 %V 16 %N 1 %R %U
ISNAD CELEBİOGLU, Hasan Ufuk , ÇELEBİ, Busenur , ERDEN, Yavuz , EVİN, Emre , ADALI, Orhan . "Cytotoxic Effects of Probiotic Bacteria Lactobacillus acidophilus and Lactobacillus rhamnosus Grown in the Presence of Oleuropein on Human Prostate Cancer Cells". Celal Bayar University Journal of Science 16 / 1 (Mart 2020): 55-59 .
AMA CELEBİOGLU H , ÇELEBİ B , ERDEN Y , EVİN E , ADALI O . Cytotoxic Effects of Probiotic Bacteria Lactobacillus acidophilus and Lactobacillus rhamnosus Grown in the Presence of Oleuropein on Human Prostate Cancer Cells. Celal Bayar Univ J Sci. 2020; 16(1): 55-59.
Vancouver CELEBİOGLU H , ÇELEBİ B , ERDEN Y , EVİN E , ADALI O . Cytotoxic Effects of Probiotic Bacteria Lactobacillus acidophilus and Lactobacillus rhamnosus Grown in the Presence of Oleuropein on Human Prostate Cancer Cells. Celal Bayar University Journal of Science. 2020; 16(1): 59-55.