The Antioxidant Effect Of Gallic Acid On The Damage Caused By Immobilization Stress To The Male Reproductive System

Öz

Aim; To assess the potential of gallic acid in stress-induced testicular damage and its impact on oxidative stress markers Method; Twenty-eight male Sprague-Dawley rats were divided into four groups: Control (C), Gallic Acid (GA), Chronic Stress (CS), and Chronic Stress + Gallic Acid (CS+GA). The CS group experienced 6-hour immobilization stress for 15 days, while the GA group received 20 mg/kg oral gallic acid daily for 15 days. The CS+GA group received gallic acid before exposure to chronic immobility stress. Histological, immunohistochemical and biochemical analyses were conducted, and data were analyzed using the SPSS program. Results; Normal testicular morphology was observed in the Control and Gallic Acid groups. The Chronic Stress group exhibited impaired seminiferous tubules and increased oxidative stress markers. However, damage decreased in the Chronic Stress + Gallic Acid group, showing restored blood-testis barrier integrity. Conclusion; Gallic acid demonstrated potential in reducing stress-induced testicular damage, suggesting its potential as a supportive agent in male infertility of unknown etiology.

Anahtar Kelimeler

• Male Infertility
• Immobilization Stress
• Oxidative Stress
• Antioxidant
• Gallic Acid

Gallik Asitin Hareketsizlik Stresinin Erkek Üreme Sisteminde Oluşturduğu Hasar Üzerine Antioksidan Etkisi

Öz

Öz Giriş ve Amaç: Bu çalışmada, gallik asidin stres kaynaklı testis hasarına karşı olası koruyucu etkisi ile oksidatif stres belirteçleri üzerindeki rolü araştırılmıştır. Gereç ve Yöntemler: Toplam 28 adet erkek Sprague-Dawley sıçan dört gruba ayrıldı: Kontrol (C), Gallik Asit (GA), Kronik Stres (CS) ve Kronik Stres + Gallik Asit (CS+GA). CS grubuna, 15 gün boyunca her gün 6 saat süren hareketsizlik stresi uygulandı. GA grubuna 15 gün boyunca günde 20 mg/kg gallik asit oral yolla verildi. CS+GA grubuna gallik asit, stres protokolünden hemen önce verildi. Histolojik, immünohistokimyasal ve biyokimyasal analizler yapıldı ve elde edilen veriler SPSS programı kullanılarak analiz edildi. Bulgular: Kontrol ve GA gruplarında normal testis morfolojisi gözlemlendi. CS grubunda, seminifer tübüllerde bozulma ve oksidatif stres belirteçlerinde artış saptandı. CS+GA grubunda ise bu hasarın azaldığı ve kan-testis bariyerinin bütünlüğünün kısmen korunduğu tespit edildi. Sonuç: Gallik asit, stres kaynaklı testis hasarını azaltmada koruyucu etki göstermiştir. Bu etkisiyle, nedeni açıklanamayan erkek infertilitesine yönelik destekleyici bir ajan olarak potansiyel taşıyabilir.

Anahtar Kelimeler

• Erkek İnfertilitesi
• Hareketsizlik Stresi
• Oksitatif Stres
• Antioksidan
• Gallik Asit

Kaynakça

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