Antioxidant Characteristic of Prophylactic Hypericum Perforatum Oil Administration in a Rabbit Model of Palatal Mucosal Injury

Yıl 2020, Cilt: 7 Sayı: 1, 55 - 64, 31.03.2020

Şadiye Günpınar , Olgu Alperat Kılıç Mehmet Tosun Tülin Fırat

https://doi.org/10.34087/cbusbed.543107

Cited By: 2

Öz

Objective: This study was aimed to
determine the antioxidant and soft tissue wound healing properties of
prophylactic (p) application of topical Hypericum
perforatum (HP)
oil in rabbits.

Materials
and Methods: Thirty-six
New Zealand albino rabbits were randomly classified as p-HP oil (test, n = 18) and p-olive oil (control, n = 18) groups. Each groups of rabbits
were topically pretreated with either HP or olive oil before the surgery. Gingival biopsies were excised and
analyzed for re-epithelialization (RE) and granulation
tissue maturation (GTM) and clinical appearance were scored for erythema, epithelial confluence and
general wound appearance on days 3, 7 and 14 after surgery. Levels of VEGF and FGF-2
were assessed
immunohistochemically. Tissue catalase (CAT) and malondialdehyde
(MDA) levels were measured using
ELISA.

Results: Higher epithelial confluence (on
the 7^th day) and general wound appearance scores (on the 7^th and
14^th days)
were determined in p-HP oil than that of p-olive oil group (p<0.05). On the other hand RE
scores did not differ between groups (p>0.05). The number of positively stained FGF-2
cells were higher in the test than that of control (p<0.05) but there were no
significant differences between the groups for the number of positively stained VEGF vessels. p-HP oil treatment increased the tissue CAT
levels and reduced the MDA levels compared to p-olive oil (p<0.05).

Conclusions: The results obtained from this study indicated that
topical p-HP oil treatment has antioxidant effects and may be used to accelerate the secondary
wound healing compared to its base, olive oil.

Anahtar Kelimeler

catalase, hypericum perforatum oil, malondialdehyde, olive oil, wound healing

Palatal Mukozal Hasar Oluşturulan Tavşanlarda Profilaktik Hypericum Perforatum Yağ Uygulamasının Antioksidan Özellikleri

Öz

Amaç:
Bu
çalışmada, topikal Hypericum perforatum (HP) yağının
tavşanlarda profilaktik olarak uygulanmasının antioksidan ve yumuşak doku yara
iyileştirici özelliklerini belirlemek amaçlandı.

Gereç ve Yöntem:
Otuz altı Yeni Zelanda albino tavşanı rastgele p-HP yağı (test, n = 18) ve
p-zeytin yağı (kontrol, n = 18) gruplarına ayrıldı. Her bir grup, cerrahi
öncesi topikal olarak HP veya zeytinyağı ile ön işleme tabi tutuldu. Dişeti
biyopsileri alınarak re-epitelizasyon (RE) ve granülasyon dokusu olgunlaşması
(GTM) açısından analiz edildi. Klinik görünüm cerrahi sonrası 3., 7. ve 14.
günlerde eritem, epitel birleşimi ve genel yara görünümü açısından skorlandı. VEGF
ve FGF-2 seviyeleri immünohistokimyasal olarak değerlendirildi. Doku katalaz
(CAT) ve malondialdehit (MDA) seviyeleri ELISA yöntemi ile ölçüldü.

Bulgular: Epitelyal
birleşme (7. günde) ve genel yara görünüm skorlarının (7. ve 14. günlerde) p-HP
yağı grubunda, p-zeytinyağı grubundakinden daha yüksek olduğu tespit edildi (p
<0.05). Diğer taraftan, RE skorları gruplar arasında farklılık göstermedi
(p> 0.05). Pozitif olarak boyanmış FGF-2 hücrelerinin sayısı test grubunda
kontrol grubundan daha yüksekti (p <0.05), fakat gruplar arasında pozitif
boyanmış VEGF damar sayısı açısından anlamlı bir farklılık yoktu. p-HP yağı
tedavisi, p-zeytinyağı uygulamasına göre doku CAT seviyelerini arttırdı ve MDA
seviyelerini düşürdü (p <0.05).

Sonuçlar:
Bu çalışmadan elde edilen sonuçlar, topikal p-HP yağı tedavisinin, içeriğindeki
zeytinyağına göre antioksidan etkilere sahip olduğunu ve ikincil yara
iyileşmesini hızlandırmak için kullanılabileceğini göstermiştir.

Anahtar Kelimeler

Katalaz, hypericum perforatum yağı, malondialdehit, zeytinyağı, yara iyileşmesi

Kaynakça

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